Association Between Diabetics and Intestinal Cancer With the Risk of Mutation in Cd38 Gene in Iranian Population

Abstract

BACKGROUND: Intestinal cancer often occurs in type 2 diabetic patients. The concept of increasing insulin levels and insulin-like growth factor in the blood with type 2 diabetes are stimulated with the growth and depletion of cloned cell walls, and the continuation of this process leads to the cellular deformation. This is the evidence for intestinal cancer in type 2 diabetes in population. OBJECTIVE: In this study, we aimed to find out the relationship between diabetics and intestinal cancer based on CD38 gene mutation. METHODS: Samples were collected from 200 population including normal and case ones. PCR products related to rs 6449181 of CD38 gene was amplified with ARMS-PCR technique, and a 420-bp sharp banding was observed as well. According three ARMS-PCR techniques, three primers were designed by oligo7 software. Primers include F1, F2 and R (amplifying for normal, mutant and reverse primer respectively). RESULTS: This band was observed using a primer F1 that carries the wild type nucleotide using a primer, and when it is used with the F2 primer, it brings the mutant primer to populations of patients with diabetes and diabetes-cancer. In addition, the clinical results including body mass index, blood glucose and insulin level were analyzed. The means ±SD and Tuckey’s post hoc test were significant between the clinical characterization parameters between cases and healthy populations. The allelic gene frequencies and Hardy-Weinberg equilibrium between nucleotides were evaluated, and the significant level between the alleles and gene frequencies was observed. CONCLUSION: In general, the current study found that there is a relationship between diabetes and intestinal cancer among the studied populations.

An in vivo investigation on the wound-healing activity of Specific ligand for the innate repair receptor, ARA290, using a diabetic animal model

“…To induce the diabetes mellitus experimental model, animals were injected via single intraperitoneal injection with STZ (60 mg/kg) freshly prepared in a cold 0.1 M sodium citrate buffer (pH 4.5) prior to fasting for approximately 16 h. After 72 h, the blood glucose level was estimated using standard glucometer to determine hyperglycemia development. Animals with blood glucose level above 250mg/dL were considered diabetic and were used as wound models [32,33].…”