Orbital retinoblastoma: case report

Mabtum, Eduardo Darahem; Bonanomi, Maria Teresa Brizzi Chizzotti; Lima, Patrícia Picciarelli de; Almeida, Maria Tereza Assis de

doi:10.1590/s0004-27492013000400013

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 30 However, some patients that progress to extraocular dissemination and metastasis are not predicted by these clinical and histopathologic features. 31 Thus, it is necessary to search for additional markers that more accurately predict high risk retinoblastoma. Herein, we observed significantly high UBE2T expression in retinoblastoma and the closely positive association of high UBE2T expression with HRPFs, PDRB, higher grade of IIRC, and pTNM staging.…”

Section: Discussionmentioning

confidence: 99%

UBE2T/STAT3 Signaling Promotes the Proliferation and Tumorigenesis in Retinoblastoma

Cui

Hong

et al. 2022

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

Purpose The purpose of this paper was to investigate the expression and function of Ubiquitin-conjugating enzyme 2T (UBE2T), a human E2 ubiquitin-conjugating enzyme, in human retinoblastoma. Methods The expression of UBE2T in normal retina and retinoblastoma was analyzed using the Gene Expression Omnibus (GEO) databases, and its expression was immunohistochemically evaluated in 29 retinoblastoma sections and 5 normal retinas. Then CCK-8, flow cytometry, RNA-sequencing analysis, and in vivo assays were performed to explore the exact role of UBE2T in retinoblastoma. Results We found that retinoblastoma showed higher UBE2T expression than normal retina in GEO datasets and tissues. The immunoreactive score of UBE2T ≥4 was associated with group E in IIRC, T2-T4b in pTNM staging, poorly differentiated retinoblastoma, and high-risk histopathological factors. Knockdown of UBE2T reduced the cell viability, increased the apoptosis cells and G0/G1 cells, and inhibited subcutaneous tumor growth in vivo. Mechanistic studies showed that UBE2T knockdown induced down-regulation of phosphorylation of STAT3 and its downstream genes in vitro and in vivo. Rescue assays confirmed that STAT3 signaling pathway was involved in the effect of reduced cell viability, elevated apoptosis cells, and G0/G1 cells mediated by UBE2T knockdown. Conclusions Our data indicate that UBE2T significantly participates in the proliferation of retinoblastoma via the STAT3 signaling pathway, suggesting the potential of UBE2T as a therapeutic target for retinoblastoma treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

UBE2T/STAT3 Signaling Promotes the Proliferation and Tumorigenesis in Retinoblastoma

Cui

Hong

et al. 2022

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…11e13 However, a small percentage of retinoblastoma cases that progress to metastasis or death are not captured by these well-validated clinical and histopathologic indicators, prompting a search for additional histologic and genetic markers capable of more accurately predicting highrisk retinoblastoma and the need for chemotherapy. 14 Cellular anaplasia recently was identified as an additional histopathologic indicator complementary to HRPFs in detecting high-risk retinoblastoma. 15 Anaplasia, an entity similar to but distinct from cellular differentiation, is defined by specific and measurable cytologic features such as pleomorphism, hyperchromatic nuclei, and a high nucleus-to-cytoplasm ratio.…”

mentioning

confidence: 99%

Distinct Gene Expression Profiles Define Anaplastic Grade in Retinoblastoma

Hudson

Mendoza

Hudson

et al. 2018

The American Journal of Pathology

View full text Add to dashboard Cite

Morbidity and mortality associated with retinoblastoma have decreased drastically in recent decades, in large part owing to better prediction of high-risk disease and appropriate treatment stratification. High-risk histopathologic features and severe anaplasia both predict the need for more aggressive treatment; however, not all centers are able to assess tumor samples easily for the degree of anaplasia. Instead, identification of genetic signatures that are able to distinguish among anaplastic grades and thus predict high- versus low-risk retinoblastoma would facilitate appropriate risk stratification in a wider patient population. A better understanding of genes dysregulated in anaplasia also would yield valuable insights into pathways underlying the development of more severe retinoblastoma. Here, we present the histopathologic and gene expression analysis of 28 retinoblastoma cases using microarray analysis. Tumors of differing anaplastic grade show clear differential gene expression, with significant dysregulation of unique genes and pathways in severe anaplasia. Photoreceptor and nucleoporin expression in particular are identified as highly dysregulated in severe anaplasia and suggest particular cellular processes contributing to the development of increased retinoblastoma severity. A limited set of highly differentially expressed genes also are able to predict severe anaplasia accurately in our data set. Together, these data contribute to the understanding of the development of anaplasia and facilitate the identification of genetic markers of high-risk retinoblastoma.

show abstract

Biomarkers in retinoblastoma

Sun

Shao

et al. 2020

Int J Ophthalmol

View full text Add to dashboard Cite

Orbital retinoblastoma: case report

Cited by 4 publications

References 15 publications

UBE2T/STAT3 Signaling Promotes the Proliferation and Tumorigenesis in Retinoblastoma

UBE2T/STAT3 Signaling Promotes the Proliferation and Tumorigenesis in Retinoblastoma

Distinct Gene Expression Profiles Define Anaplastic Grade in Retinoblastoma

Biomarkers in retinoblastoma

Contact Info

Product

Resources

About