Freeze-drying as an alternative method of human sclera preservation

Frota, Ana Carolina de Arantes; Filho, Acácio Alves de Souza Lima; Dias, Alexandra; Lourenço, Andréia Cristina dos Santos; Antecka, E.; Burnier, Miguel N.

doi:10.1590/s0004-27492008000200002

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…As the numbers of cases of cardiovascular disease steadily grow, grafting as a therapeutic method has been demonstrated to be effective, and millions of tissues and organs are demanded (1). However, as there is a shortage of donor tissues and organs and low graft efficiency, the clinical application of aorta grafting has been restrained (2)(3)(4). Since the 1980s, a variety of substitutes have been used for aorta grafting.…”

Section: Introductionmentioning

confidence: 99%

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The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts

Cao

Liu

et al. 2012

Molecular Medicine Reports

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Vacuum freeze-dried aorta is a satisfactory material for blood vessel grafting. Previous studies have focused on immunity, however, vacuum freeze-drying is a complicated process of heat and mass transfer, and adopting a programmed cooling process may more completely preserve the mechanical properties of the blood vessels. Irradiation, as a method of removing pathogens, lowers the antigenic activity of the blood vessels. In our study, vacuum freeze-drying combined with radiation was used as a treatment for porcine aorta prior to grafting, aimed at deactivating endogenous retrovirus, shielding masses of endothelial cells and lowering the immunogenicity of the blood vessels. As for the mechanical properties, compared with normal aorta, the maximum axial tensile stress (ATS) decreased by 20%, the maximum circumferential tensile stress (CTS) increased by 30% and the maximum puncture stress (PT) decreased by 20%. Our results revealed that 2 months after of grafting, the host cells had migrated into the graft tissue and propagated to initiate endothelialization, the inflammatory reaction was abated and the PT had returned to normal levels.

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Section: Introductionmentioning

confidence: 99%

“…Detection of porcine endogenous retrovirus. The band for the virus was revealed in (1) the porcine endogenous retrovirus C genome, (2) the normal aorta genome and (3) the genome of the reconstituted aorta treated by vacuum freeze-drying (4). No band was detected in the genome of the reconstituted aorta treated by vacuum freeze-drying and radiation.…”

mentioning

confidence: 97%

The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts

Cao

Liu

et al. 2012

Molecular Medicine Reports

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The use of donor scleral patch in ophthalmic surgery

et al. 2016

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Scleral tissue has been in use in ophthalmology for many years although indications for use have varied. We retrospectively reviewed scleral transplant tissue requests over a 12 month period at a local eye bank and confirmed a small but significant demand for the use of scleral tissue. Iatrogenic surgical complications are the primary indication for use. Our understanding of the indications and outcomes of scleral graft procedures is derived from case reports and small cohort series. We reviewed the current literature on existing indications for its use and discuss the relative outcomes. To our knowledge this represents the first review of scleral transplant indications and further summarises usage rates in the Lions NSW Eye Bank which may provide practical information for those surgeons who use scleral tissue and Eye Banks who supply it.

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A Case of Fusarium Deep Keratitis Following Scleral Graft

Kim

Chun

2010

J Korean Ophthalmol Soc

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Freeze-drying as an alternative method of human sclera preservation

Cited by 3 publications

References 15 publications

The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts

The effect of vacuum freeze-drying and radiation on allogeneic aorta grafts

The use of donor scleral patch in ophthalmic surgery

A Case of Fusarium Deep Keratitis Following Scleral Graft

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