Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

Silva, Maísa; Lima, Wanderson Geraldo de; Pedrosa, Maria Lúcia

doi:10.1590/s0004-27302011000100006

Cited by 25 publications

(18 citation statements)

References 31 publications

(24 reference statements)

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“…Similar results were obtained by Silva et al (2011), who observed partial destruction of pancreatic β-cells, and the STZ cytotoxicity in β-cells was proportional to the administered dose. The cytotoxicity effects of STZ on the pancreatic β-cells are due to its similarity with Figure 6.…”

Section: Resultssupporting

confidence: 88%

Effect of hydroethanolic extract from Calophyllum brasiliense Cambess on streptozotocin induced diabetic rats

Carvalho¹,

Lacerda²,

Ferreira³

et al. 2016

Afr. J. Pharm. Pharmacol.

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Calophyllum brasiliense (Cb) belongs to the Clusiaceae family and it is generally used for diabetes treatment. The aim of this study was to evaluate the anti-diabetic effect of Cb's hydroethanolic extract (CBE) on diabetic induced rats by streptozotocin and evaluate the content of polyphenols and tannins of the extract. The polyphenols and tannins evaluation in the extract were determinated by a spectrophotometer in 760 nm and isocratic HPLC system and reverse phase column (C18). The induction of diabetes was performed by intraperitoneal injection of streptozotocin (55 mg/kg) and was confirmed by a histopathological analysis. The total content of polyphenols and tannins (gallic acid) in CBE was 0.025 ± 0.0028 mg/mL (4.77%) and 8.262 ± 0.417 µg/mL, respectively. According to the oral glucose tolerance test that was performed both in normal and diabetic mice, the treatment with 500 mg/kg of CBE appeared to significantly reduce the blood glucose levels compared to the untreated group (P < 0.001). The treatment of diabetic mice with 500 mg/kg of CBE for 30 days significantly improved diabetes clinical symptoms (polydipsia, polyuria, polyphagia and weight loss) (P < 0.001). After the urinary glucose analysis, it was found that the treatment with 500 mg/kg of CBE significantly decreased the urinary glucose levels at an average of 177.55 ± 17.8 mg/dL (32.40%) (P < 0.001). In relation to the blood glucosemeasurements, it was shown that the groups treated with 500 mg/kg CBE and 3 mg/kg of Glibenclamide had significantly lower levels of blood glucose when compared to nontreated group (P < 0.001) 24.09% (143.36 ± 19.6 mg/dL) and 36.04% (200.08 ± 14.9 mg/dL), respectively). The histopathological analysis revealed an increase in the number of endocrine cells in the islets of Langerhans in the groups treated with CBE500 and insulin. Therefore, it was concluded that the treatment with 500 mg/kg of CBE exhibited anti-diabetic activity.

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Section: Resultssupporting

confidence: 88%

Effect of hydroethanolic extract from Calophyllum brasiliense Cambess on streptozotocin induced diabetic rats

Carvalho¹,

Lacerda²,

Ferreira³

et al. 2016

Afr. J. Pharm. Pharmacol.

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“…The reduced glutathione plays an important role in protecting the sulfhydryl groups in membranes, in addition to protecting the cell from free radicals and reactive strains of oxygen, exerting an important function against the oxidative stress of the diabetes (30). The NPSHG are compounds that can be modified by the action of free radicals, and therefore considered as oxidative stress measures (31). Therefore, the results found here evidence a beneficial effect of the treatments with insulin and multimixture, being the effect of insulin greater than that of the multimixture.…”

Section: Resultsmentioning

confidence: 99%

Hepatic function and antioxidant activity in diabetic rats subjected to diet supplemented with multimixtture

Teixeira

Martins²,

Soares

et al. 2016

Nutr Hosp

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ResumenIntroducción: alimentos y componentes de la dieta han recibido considerable atención como recursos auxiliares en el control de las enfermedades crónicas, como la diabetes. Este estudio evaluó el efecto de la dieta suplementada con base de linaza multimezcla, sésamo, avena y semillas de girasol en la función hepática y la actividad antioxidante de ratas diabéticas. Métodos: las ratas macho fueron divididas en grupos de siete animales: control de la diabetes (CD), diabéticos sometidos a dieta multimezcla (DM), diabéticos con insulina (DI), y control normal (CN). El tratamiento se inició en el quinto día después de la inducción de la diabetes con estreptozotocina 40 mg/kg i.v. en tampón citrato y se mantuvo durante 50 días. Resultados: la dieta extracto etéreo complementado tenía el contenido fenólico más alto (p < 0,05) que los otros extractos de la suplementación de la dieta y el extracto de éter de la comida estándar. No hubo diferencia en la actividad antioxidante in vitro de la alimentación usada. A medida que las transaminasas, los niveles de ALT signifi cativamente mayor (p < 0,05) estuvieron presentes en el grupo de diabéticos en comparación con CN, el peso relativo del hígado en el grupo de diabéticos fue signifi cativamente mayor (p < 0,001) en comparación con el grupo CN. Los grupos sulfhidrilo de los niveles de proteína no fueron signifi cativamente mayores (p < 0,05) en los grupos F e I comparados con CN y CD. El tratamiento con insulina resultó en una mejora de la actividad antioxidante con respecto a la catalasa hepática. Conclusión: la suplementación con multimezcla no mejora el control metabólico de la diabetes. El tratamiento con multimezcla aislado mostró una mejora en la actividad antioxidante en el tejido hepático, como se evidencia por el aumento de los niveles de grupos sulfhidrilo no proteicos. AbstractIntroduction: Food and dietetic components have received considerable attention as auxiliary feeding resources on controlling of chronic non-transmissible diseases, among them diabetes. This study evaluated the effect of supplementary diet with multimixture based on linseed, sesame, oats and sunfl ower seeds on the hepatic function and antioxidant activity of diabetic rats. Methods: Male rats were distributed on groups of seven animals: diabetic control (DC), diabetics subject to multimixture diet (DM), diabetic with insulin (DI), and normal control (NC). The treatment was started on the 5 th day after diabetes induction with 40 mg/kg i.v. streptozotocin on tampon citrate and kept during 50 days. Results: The ethereal extract of the supplemented diet showed a higher content of phenolics (p < 0.05) compared to other extracts of the supplemented diet and the ethereal extract of the standard diet. There was no difference in antioxidant in vitro activity of the utilized diets. Concerning to transaminases, signifi cant bigger ALT levels (p < 0.05) were present on diabetic groups compared to NC. The relative liver weight on diabetic groups was signifi cantly higher (p < 0.001) compared to NC ...

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“…In diabetes experimental models, it has been reported that PON1 enzymatic activity is decreased [6,7] and reduced PON1 activity was suggested to play a role in the severity of coronary atherosclerosis [2]. Nonetheless, oxidative stress pathways have also been proposed to act in this process, with increased reactive oxygen species (ROS) production and/or impaired antioxidant defense, which may in turn lead to excessive peroxidation of polyunsaturated fatty acids contained in LDL particles.…”

Section: Resultsmentioning

confidence: 99%

Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity

et al. 2012

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Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P < 0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.

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Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

Cited by 25 publications

References 31 publications

Effect of hydroethanolic extract from Calophyllum brasiliense Cambess on streptozotocin induced diabetic rats

Effect of hydroethanolic extract from Calophyllum brasiliense Cambess on streptozotocin induced diabetic rats

Hepatic function and antioxidant activity in diabetic rats subjected to diet supplemented with multimixtture

Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity

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