Marcadores Bioquímicos da Remodelação Óssea na Prática Clínica

Saraiva, Gabriela Luporini; Lazaretti‐Castro, Marise

doi:10.1590/s0004-27302002000100010

Cited by 32 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The amount of OC in the supernatant was measured by ELISA, which revealed the presence of protein in the supernatant, the OC was detected from the seventh reaching its maximum peak in the twenty-first day and soon after we were able to observe its Although bone marrow cells have been used for this purpose, in tissue engineering these cells require a specialized culture and a purification procedure that may result in a cell population that is smaller than desired 23 . Non-osteogenic cells, such as dermal fibroblasts, are a potential cell source because they are easily obtainable from autologous tissues and can rapidly expand in vitro 13,[20][21][22]24 .…”

Section: Resultsmentioning

confidence: 99%

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

et al. 2014

View full text Add to dashboard Cite

PURPOSE:To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS:We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycerophosphate and 10 nmol/L of dexamethasone); and OSTEOG/TGF-β1 (osteogenic medium with 10 ng/ml of TGF-β1). Alkaline phosphatase (ALP) activity and the amount of osteocalcin (OC) in the supernatant, as well as the capability to form calcium phosphate deposits, were analysed for 28 days RESULTS: There were significant differences (p<0.05) between CONTROL and TGF-β1 groups in comparison with OSTEOG and OSTEOG/TGF-β1 groups in the ALP activity and OC amount. Although, both osteogenic groups had the same behavior with regard the expression curve during the experimental time, the OSTEOG/TGF-β1 group achieved significantly higher ALP and OC levels and showed no significant difference in the levels of mineralized deposits and in comparison with the levels found in the OSTEOG group. CONCLUSION:The addition of transforming growth factor beta 1 to the osteogenic culture medium increased the activity of alkaline phosphatase and the amount of osteocalcin, but TGF-β1 did not alter the presence of mineralized calcium phosphate deposits.

show abstract

Section: Resultsmentioning

confidence: 99%

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Campos e cols. encontraram resultados semelhantes aos nossos para a ingestão de cálcio em mulheres submetidas à DGYR (23,26).…”

Section: Discussionunclassified

Alterações de parâmetros relacionados ao metabolismo ósseo em mulheres submetidas à derivação gástrica em Y de Roux

Santos

Souza

Fonseca

et al. 2012

Arq Bras Endocrinol Metab

Self Cite

View full text Add to dashboard Cite

RESUMOObjetivo: Avaliar o metabolismo ósseo e a densidade mineral óssea (DMO) em mulheres adultas pós-derivação gástrica em Y de Roux (DGYR). Sujeitos e métodos: Estudo transversal com 48 mulheres submetidas a DGYR há três anos e 41 saudáveis. Dados obtidos: índice de massa corporal (IMC), atividade física, consumo alimentar e DMO da coluna lombar, colo e fêmur total. Dosagem de cálcio, fósforo, magnésio, albumina, fosfatase alcalina, telopeptídeo-C (CTX), paratormônio (PTH), 25-hidroxivitamina D (25OHD), osteocalcina e cálcio urinário. Resultados: Maiores alterações no grupo DGYR observadas nos níveis de osteocalcina (p < 0,001), CTX (p < 0,001) e PTH (p < 0,001). Deficiência de 25OHD foi a mais frequente no grupo DGYR (p = 0,010). Deficiência/insuficiência de 25OHD associou-se com hiperparatiroidismo secundário (p = 0,025). Não houve diferença entre os grupos em relação à DMO. A ingestão de energia (p = 0,036) e proteína (p = 0,004) foi maior no grupo controle. Conclusão: Em mulheres pós-DGYR, encontraram-se alta frequência de deficiência de vitamina D, hiperparatireoidismo secundário e elevação nos marcadores de remodelação óssea, sem alteração na DMO quando comparado com o grupo controle não obeso. Arq Bras Endocrinol Metab. 2012;56(6):376-82 Descritores Cirurgia bariátrica; densidade mineral óssea; vitamina D; hiperparatiroidismo secundário; anastomose em-Y de Roux ABSTRACT Objective: To evaluate bone turnover markers and bone mineral density (BMD) in women after Roux-en-Y (RYGB) gastric bypass. Subjects and methods: In a cross-sectional study, 48 women post-RYGB after three years, and 41 healthy women were evaluated. Evaluations: body mass index (BMI); physical activity; food intake; serum levels of calcium, phosphorus, magnesium, alkaline phosphatase, C-terminal telopeptide (CTX), intact parathyroid hormone (PTH), 25-hydroxyvitamin D (25OHD), osteocalcin, urinary calcium and BMD. Results: Significantly higher levels were observed for osteocalcin (p < 0.001), CTX (p < 0.001), and PTH (p < 0.001) in the RYGB group when compared with the control group; 25OHD deficiency/insufficiency was more frequent in the RYGB group (p = 0.010), even after adjusted for nutritional status, and it was associated with secondary hyperparathyroidism (p = 0.025); there was no difference in BMD between the groups. Energy (p = 0.036) and protein intake (p = 0.004) were lower in the RYGB group. Conclusion: Patients submitted to RYGB showed a significantly higher frequency of vitamin D deficiency, secondary hyperparathyroidism, and increase in bone remodeling markers, with no difference in BMD status. Arq Bras Endocrinol Metab. 2012;56(6):376-82

show abstract

“…[19][20][21][22][23][24] Visto que falhas na osteogênese pode resultar na má formação óssea, foi necessário a pesquisa e desenvolvimento de materiais que apresentam características que os tornam capazes de corrigir e/ou minimizar tais danos. estimulando a formação óssea (osteoblasto) e inibe a reabsorção óssea (osteoclasto), aumentando a massa óssea.…”

Section: Tecido óSseounclassified

The Influence of Zinco on Bone Repair: A Literature Review

Souza¹,

Santos

Marques

et al. 2018

Rev. Virtual Quim.

View full text Add to dashboard Cite

Abstract:The challenge in the treatment of bone defects, increased the search for an ideal material, which promotes, more quickly, the regeneration of the bone tissue. Obviously the ideal material does not exist, what has characteristics closer is the autogenous bone, but still has restrictions as the quantity acquired. Due to these shortcomings new materials are being developed. The biomaterials containing zinc in their structure were highlighted for a better repair of the injured bone. The present study analyzed the influence of zinc on bone repair, according to a review study and concluded that the element zinc is fundamental in the protein synthesis of several enzymes that have indirect binding to bone repair. The action of zinc is to stimulate the synthesis of proteins and consequent action on the enzymes / hormones related to bone growth. In addition, zinc is an inhibitory agent of osteoclast cells, which are related to bone resorption.Keywords: Zinc; Biomaterials; bone formation. ResumoO desafio no tratamento de defeitos ósseos, aumentaram a procura por um material ideal, o qual promova, com maior celeridade, a regeneração do tecido ósseo. Obviamente o material ideal não existe, o que tem características mais próximas é o osso autógeno, mais ainda possui restrições quanto a quantidade adquirida. Devido a estas deficiências estão sendo desenvolvidos novos materiais. Os biomateriais contendo zinco em sua estrutura se destacaram para uma melhor reparação do ósseo lesionado. O presente estudo analisou a influência do zinco no reparo ósseo, segundo um estudo revisional e conclui-se que o elemento zinco é fundamental na síntese proteica, de diversas enzimas que têm ligação indireta para o reparo ósseo. A ação do zinco é a de estimular a síntese de proteínas e consequente ação nas enzimas/hormônios relacionadas com o crescimento ósseo. Além disso, o zinco é um agente inibitório das células osteoclásticas, que estão relacionadas a reabsorção óssea.Palavras-chave: Zinco; Biomateriais; formação óssea.

show abstract

Marcadores Bioquímicos da Remodelação Óssea na Prática Clínica

Cited by 32 publications

References 20 publications

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

Alterações de parâmetros relacionados ao metabolismo ósseo em mulheres submetidas à derivação gástrica em Y de Roux

The Influence of Zinco on Bone Repair: A Literature Review

Contact Info

Product

Resources

About