Effect of l-glutamine on myenteric neuron and of the mucous of the ileum of diabetic rats

Abstract

The objective of this work was to investigate the effect of the L-glutamine supplementation to prevent -diabetes induced changes in myenteric neurons and also to verify the effect on the mucosa of the ileum of Wistar rats. The animals were divided in five groups (n = 5): untreated normoglycaemic (UN), normoglycaemic treated with L-glutamine (NG), untreated diabetics (UD), diabetics treated with L-glutamine, starting on the 4 th (DG4) or 45 th day following diabetes induction (DG45). The amino acid was added to the diet at 1%. The density and size of neurons, the metaphasic index in the crypt, the height of the villus, the depth of the crypt and the number of globet cells were determined. There was no difference in the neuronal density and in the cellular body area of the myosin-stained myenteric neurons of groups DG4 and DG45 when compared to group D. The metaphase index and the number of goblet cells showed no significant differences when all groups were compared (P > 0.05). The villi height of groups DG4 and dG45 were 45.5% (P < 0.05) and 32.4% (P > 0.05) higher than those in group ud, respectively. The analyzed crypts showed similar depth for all studied groups.

Supplementation with L-Glutamine and L-Alanyl-L-Glutamine Changes Biochemical Parameters and Jejunum Morphophysiology in Type 1 Diabetic Wistar Rats

“…We observed not only hyperglycemia ( Table 1 ) but also typical manifestations of the diabetic condition, such as hyperphagia, lower body mass [ 7 , 19 ], polyuria, polydipsia, and diarrhea [ 20 ].…”

“…Daily supplementation with L-glutamine by gavave during 30 days did not affect the physiopathological changes induced by streptozotocin (D group vs. DG group) and had no effect on the condition in the non-diabetic animals treated with L-glutamine (C group vs. CG group), as found by Tronchini et al . [ 19 ] and Tsai et al . [ 22 ].…”

“…[ 22 ]. Interestingly, in these studies [ 19 , 22 ] they used the same period of supplementation but the animals received L-glutamine in the chow.…”

Glutamine or Glutamine Dipeptide Supplementation Improves Gluconeogenesis and Liver Glycogenosis in Type 1 Diabetic Rats

“…Dyslipidemias are common in diabetic individuals, as experimentally demonstrated by Rosa et al [19] and Tronchini et al [39]. These alterations can favor the appearance of lipid infiltrations in the hepatic tissue [40], a condition known as hepatic steatosis.…”

The effects of Yam (Dioscorea bulbifera) intake on small intestine morphology in streptozotocin‐induced diabetic rats

“…The disaccharidases are located in the villi region of small intestine (Betton, ). Furthermore, diabetic rats showed hyperplasia and hypertrophy of the mucosa, associated with increase on the villus thickness in the duodenum, jejunum and ileum (Tronchini et al ., ; Zhao et al ., ,b). In the present study, both diabetic rat groups showed increase of villus and mucosa thickness.…”