Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Abstract

This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders (pH 6.1; 7.8 and 8.2), two concentrations of dimethyl sulfoxide (dMSO, 5 and 10%) and three cooling rates (-90; -60 and -30°C.min ) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes (1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time (P < 0.05) was achieved by combining extender C (ph 8.2) with dMSO (10%) and cooling rate of -60°C.min -1 (P < 0.05). The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

“…Red snapper cryopreserved sperm yielded fertilization rates ranging from 11% to 85%, and this variation was attributed to differences in oocyte quality (Riley et al, 2004). Moreover, high fertilization rates (90%) were observed in mangrove red snapper using cryopreserved sperm (Vuthiphandchai et al, 2009) and mutton snapper (Sanches et al, 2013). Sperm refrigerated for 48 h yielded fertilization rates greater than 50% in lane snapper (Sanches and Cerqueira, 2010).…”

“…Currently it is estimated that more than 200 species with experimentally determined cryoconservation protocols only 40 protocols are from marine species (Thirumala et al, 2006), and in Brazil had only four marine species protocols for sperm cryoconservation determined: Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Lanes et al, 2008), fat snook Centropomus parallelus (Poey, 1860) (Tiba et al, 2009), dusky grouper Epinephelus marginatus (Lowe, 1834) (Sanches et al, 2009), and mutton snapper Lutjanus analis (Cuvier, 1828) (Sanches et al, 2013). This technique is considered as an essential tool to protect endangered species or distortion, allowing the storage of their gametes in germoplasm banks.…”

“…Although sperm cryoconservation is an important strategy for the control of reproduction, few studies have focused on these techniques for species of lutjanidae, with emphasis results obtained with Lutjanus campechanus (Poey, 1860), with Lutjanus argentimaculatus (Forsskål, 1775) (Riley et al, 2004;Vuthiphandchai et al, 2009) and the mutton snapper L. analis (Sanches et al, 2013).…”

Cryoprotectants & Cryopreservation of Equine Semen: A Review of Industry Cryoprotectants and the Effects of Cryopreservation on Equine Semen Membranes

“…Species, whose semen specifically perform better following freezing with DSMO may do so because glycerol acts as a contraceptive for them. A few species that have benefited from DMSO as a primary CPA include: mice, rabbits [21] and a variety of fish-including: zebra fish [22], carp broodstock [23], seven-band grouper [24] and mutton snapper [25]. Rabbit sperm appear to do best with a substantial proportion of DSMO in relationship to glycerol for CP.…”

Detecção, controle e prevenção de fotobacteriose em cultivo de bijupirá

“…A dificuldade da obtenção de formas jovens, que era o principal gargalo para a atividade no Brasil, vem sendo solucionada com a adoção de novas tecnologias (Sanches et al, 2013b). Para diversas espécies de peixes marinhos nativas e de elevado valor econômico, seus protocolos de reprodução e/ou de larvicultura foram estabelecidos, tais como o robalo-peva (Centropomus parallelus) (Alvarez-Lajonchère & Tsuzuki, 2008), a garoupa-verdadeira (Epinephelus marginatus) (Sanches et al, 2009), o ariocó (Lutjanus synagris) (Sanches & Cerqueira, 2010) e a cioba (Lutjanus analis) (Sanches et al, 2013a). Porém, o peixe marinho que tem, atualmente, despertado maior interesse para o cultivo tem sido o bijupirá (Rachycentron canadum).…”