Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells

Abstract

Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografi na ® 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina ® 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being signifi cantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina ® 292 may cause a signifi cant increase of cytogenetic damage in bone marrow cells of mice.

Contrast-induced nephropathy in an animal model: Evaluation of novel biomarkers in blood and tissue samples

“…The second refers to chemical nature of CM, which may also induce chromosomal damage [44]. Finally, recent studies indicate the mutagenic effects of iodinated CM in animal models distant from the irradiated field [45,46].…”

Evaluation of dexamethasone and methylprednisolone administration on visceral organs prior to contrast radiography in Nigerian indigenous breed of dogs

“…Radiographic contrast media are a group of medical drugs used to improve the visibility of internal fluid, organs and structures in medical imaging, such as angiography, computed tomography, diagnostic X-ray (radiology) and in radiotherapy for treatment planning (Therapeutically in hyperthyroid patients, to rapidly correct severe hyperthyroidism prior to thyroidectomy). The currently available contrast media are indispensable in the practice of radiology, for both diagnostic and therapeutic purposes [1,2,3]. The number of medical procedures using iodinated contrast media has dramatically increased in recent decades [3].…”