Neurochemical phenotype and birthdating of specific cell populations in the chick retina

Calaza, Karin da Costa; Gardino, P.F.

doi:10.1590/s0001-37652010000300007

Cited by 22 publications

(22 citation statements)

References 99 publications

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“…The retina has all or nearly all neurotransmitter and neuromodulatory systems found at other CNS regions, including the adenosinergic (Calaza and Gardino 2010). In the retina of chick embryos, adenosine is present from early developmental stages (embryonic day 12, E12) to posthatching (Paes-de-Carvalho et al 1992).…”

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confidence: 99%

Caffeine exposure alters adenosine system and neurochemical markers during retinal development

Brito

Pereira-Figueiredo

Socodato

et al. 2016

Journal of Neurochemistry

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Evidence points to beneficial properties of caffeine in the adult central nervous system, but teratogenic effects have also been reported. Caffeine exerts most of its effects by antagonizing adenosine receptors, especially A 1 and A 2A subtypes. In this study, we evaluated the role of caffeine on the expression of components of the adenosinergic system in the developing avian retina and the impact of caffeine exposure upon specific markers for classical neurotransmitter systems. Caffeine exposure (5-30 mg/kg by in ovo injection) to 14-day-old chick embryos increased the expression of A 1 receptors and concomitantly decreased A 2A adenosine receptors expression after 48 h. Accordingly, caffeine (30 mg/kg) increased receptors from 18 and 24 h. Kinetic assays of [ 3 H]-S-(4-nitrobenzyl)-6-thioinosine binding to the equilibrative adenosine transporter ENT1 revealed an increase in B max with no changes in K d , an effect accompanied by an increase in adenosine uptake. Immunohistochemical analysis showed a decrease in retinal content of tyrosine hydroxylase, calbindin and choline acetyltransferase, but not Brn3a, after 48 h of caffeine injection. Furthermore, retinas exposed to caffeine had increased levels of phosphorylated extracellular signalregulated kinase and cAMP-response element binding protein.Overall, we show an in vivo regulation of the adenosine system, extracellular signal-regulated kinase and cAMPresponse element binding protein function and protein expression of specific neurotransmitter systems by caffeine in the developing retina.

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confidence: 99%

Caffeine exposure alters adenosine system and neurochemical markers during retinal development

Brito

Pereira-Figueiredo

Socodato

et al. 2016

Journal of Neurochemistry

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“…Dopamine is the main catecholamine found in a subtype of retinal amacrine cells located in the inner nuclear layer (INL; Reis et al, 2007 ). Emergence of amacrine cells begins at early stages (around embryonic day 3, E3) and is concluded at E9 (Prada et al, 1991 ; Calaza Kda and Gardino, 2010 ). Tyrosine hydroxylase (TH) expression is only found around E12, while D 1 /D 5 type dopaminergic receptors coupled to cyclic AMP (cAMP) production are fully functional on E7 (de Mello, 1978 ) as compared to the end of the proliferation period (Gardino et al, 1993 ).…”

Section: Introductionmentioning

confidence: 99%

Cannabinoid Receptor Type 1 Expression in the Developing Avian Retina: Morphological and Functional Correlation With the Dopaminergic System

Sampaio

Kubrusly

Colli

et al. 2018

Front. Cell. Neurosci.

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The avian retina has been used as a model to study signaling by different neuro- and gliotransmitters. It is unclear how dopaminergic and cannabinoid systems are related in the retina. Here we studied the expression of type 1 and 2 cannabinoid receptors (CB1 and CB2), as well as monoacylglycerol lipase (MAGL), the enzyme that degrades 2-arachidonoylglycerol (2-AG), during retina development. Our data show that CB1 receptor is highly expressed from embryonic day 5 (E5) until post hatched day 7 (PE7), decreasing its levels throughout development. CB1 is densely found in the ganglion cell layer (GCL) and inner plexiform layer (IPL). CB2 receptor was also found from E5 until PE7 with a decrease in its contents from E9 afterwards. CB2 was mainly present in the lamination of the IPL at PE7. MAGL is expressed in all retinal layers, mainly in the IPL and OPL from E9 to PE7 retina. CB1 and CB2 were found both in neurons and glia cells, but MAGL was only expressed in Müller glia. Older retinas (PE7) show CB1 positive cells mainly in the INL and co-expression of CB1 and tyrosine hydroxylase (TH) are shown in a few cells when both systems are mature. CB1 co-localized with TH and was heavily associated to D1 receptor labeling in primary cell cultures. Finally, cyclic AMP (cAMP) was activated by the selective D1 agonist SKF38393, and inhibited when cultures were treated with WIN55, 212–2 (WIN) in a CB1 dependent manner. The results suggest a correlation between the endocannabinoid and dopaminergic systems (DSs) during the avian retina development. Activation of CB1 limits cAMP accumulation via D1 receptor activation and may influence embryological parameters during avian retina differentiation.

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“…Each retinal cell is generated from a common population of retinal progenitor cells (RPCs) in distinct yet overlapping periods during retinogenesis (Sidman, 1961;Young, 1985). These timespans can be variable in duration, however, as observed in the developing chick retina where RPCs have been reported to be capable of generating some fates for anywhere from a few days to over a week (Calaza Kda & Gardino, 2010). To add to this complexity, RPCs have been shown through lineage analyses to be multipotent, with some cell divisions capable of generating two distinct retinal cell types (Holt et al, 1988;Turner & Cepko, 1987;Turner, Snyder, & Cepko, 1990).…”

Section: Introductionmentioning

confidence: 99%

Single cell transcriptome profiling of developing chick retinal cells

Laboissonniere

Martin

Goetz

et al. 2017

J of Comparative Neurology

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The vertebrate retina is a specialized photosensitive tissue comprised of six neuronal and one glial cell types, each of which develops in prescribed proportions at overlapping timepoints from a common progenitor pool. While each of these cells has a specific function contributing to proper vision in the mature animal, their differential representation in the retina as well as the presence of distinctive cellular subtypes makes identifying the transcriptomic signatures that lead to each retinal cell's fate determination and development challenging. We have analyzed transcriptomes from individual cells isolated from the chick retina throughout retinogenesis. While we focused our efforts on the retinal ganglion cells, our transcriptomes of developing chick cells also contained representation from multiple retinal cell types, including photoreceptors and interneurons at different stages of development. Most interesting was the identification of transcriptomes from individual mixed lineage progenitor cells in the chick as these cells offer a window into the cell fate decision-making process. Taken together, these data sets will enable us to uncover the most critical genes acting in the steps of cell fate determination and early differentiation of various retinal cell types.

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Neurochemical phenotype and birthdating of specific cell populations in the chick retina

Cited by 22 publications

References 99 publications

Caffeine exposure alters adenosine system and neurochemical markers during retinal development

Caffeine exposure alters adenosine system and neurochemical markers during retinal development

Cannabinoid Receptor Type 1 Expression in the Developing Avian Retina: Morphological and Functional Correlation With the Dopaminergic System

Single cell transcriptome profiling of developing chick retinal cells

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