Meiotic behavior, pollen morphology, interphasic nucleus pattern and karyotype description for Duranta repens (Verbenaceae) are presented. The species had semi-reticulate interphasic nucleus and in mitotic metaphase, chromosome number was 2n = 34 (32 m + 2 sm), with secondary constriction in one chromosome pair. Additionally to the A complement, we observed B chromosomes, varying to 0-3 in some cells of several individuals. Meiotic behavior was regular and pollen grains were isopolar and tricolporate, being classifi ed as prolate-spheroidal with psilate-perfurate exine ornamentation.