Erratum to ''Stereological tools in biomedical research'': an Acad Bras Cienc 75(2003): 469-486

Mandarim-de-Lacerda, Carlos Alberto

doi:10.1590/s0001-37652007000100007

Cited by 11 publications

(12 citation statements)

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“…The smooth muscle tissue was quantified by a stereological method. [10][11][12] We studied five microscopic fields chosen at random, totaling 25 test areas for each bladder for the quantitative analysis. We used the Image J software, version 1.46r, loaded with its own plug-in (http://rsb.info.nih.gov/ij/).…”

Section: Methodsmentioning

confidence: 99%

Structural study of the bladder in fetuses with prune belly syndrome

Julio

Costa

et al. 2017

Neurourology and Urodynamics

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Aims: To study the bladder structure of fetuses with prune belly syndrome (PBS). Methods:We studied three bladders obtained from three male fetuses with PBS and seven bladders from seven male fetuses without anomalies. Each bladder was dissected and embedded in paraffin, from which 5 μm thick sections were obtained and stained with Masson's trichrome (to quantify connective tissue and smooth muscle) and picrosirius red with polarization (to observe collagen). Immunohistochemistry with tubulin (Tubulin, beta III, Mouse Monoclonal Antibody) was applied to observe the bladder nerves. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs, using a grid to determine volumetric densities (Vv). Means were statistically compared using the Mann-Whitney test (P < 0.05). Results: Quantitative analysis documented that smooth muscle fibers were significantly smaller (P = 0.04) in PBS fetuses (9.67% to 17.75%, mean = 13.2%) compared to control group (13.33% to 26.56%, mean = 17.43%). The analysis of collagen fibers showed predominance of green in the control group, suggesting collagen type III presence, and predominance of red in the in PBS fetal bladders, suggesting collagen type I presence in this group. The qualitative analysis of the nerves with immunohistochemistry with tubulin showed predominance of nerves in the control group. Conclusion: The bladder in PBS had lower concentrations of smooth muscle fibers, collagen type III, and nerves. These structural alterations can be one of the factors involved in urinary tract abnormality such as distended bladder in patients with PBS.

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Section: Methodsmentioning

confidence: 99%

Structural study of the bladder in fetuses with prune belly syndrome

Julio

Costa

et al. 2017

Neurourology and Urodynamics

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“…The M42 system is composed of 21 line segments with 42 test points. The stereological parameter analyzed was volumetric density (Vv): Vv = Pp/Pt%, with Pp being the points relating to the smooth muscle, collagen, or elastic fibers, and Pt being the total test points of the test system 15,16 .…”

Section: Methodsmentioning

confidence: 99%

Structural changes of dilated pelvic ureters in adults

et al. 2014

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PURPOSE:To perform a quantitative assessment of different types of pelvic ureter tissues with chronic dilation in adults, using stereological methods. METHODS:We analyzed fragments of dilated pelvic ureters obtained from 6 patients aged between 35 and 67 years (mean, 46 years) who underwent ureteral reimplantation surgery for different reasons. The obstruction duration ranged from 27 to 180 days (mean, 93 days). The control group included fragments of normal pelvic ureters obtained during nephrectomy in 7 kidney transplant donors (age: range, 25-51 years; mean, 36 years). The volumetric density of collagen in the ureter, elastic fibers, and smooth muscle fibers was assessed. RESULTS:The volumetric density (Vv) of collagen showed no significant difference between the two groups (control: 45.3 ± 6.1; dilated: 40.8 ± 6.9; P = 0.23). A statistically significant increase in Vv of elastic fibers was observed in the dilated ureters (control: 18.4 ± 1.2; dilated: 24.6 ± 5.4; P = 0.03). A statistically significant increase in the Vv of smooth muscle fibers was observed in the dilated ureter (control: 42.0 ± 6.0; dilated: 56.2 ± 6.1; P = 0.001). CONCLUSION:The chronically dilated pelvic ureters had significantly more elastin and smooth muscle contents than the controls.

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“…Para a estimativa da densidade por área dos perfis de corpos celulares de neurônios utilizamos a metodologia de contagem aleatória simples [16][17][18][19][20] . Neste método adquirimos dois campos microscópicos aleatórios de três cortes semi seriados da área, totalizando assim seis (6) áreas analisadas por animal.…”

Section: Coleta Das Amostrasunclassified