Sodium pumps in the Malpighian tubule of Rhodnius sp.

Caruso‐Neves, Celso; Lopes, Anibal Gil

doi:10.1590/s0001-37652000000300014

Cited by 12 publications

(9 citation statements)

References 27 publications

(32 reference statements)

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“…The enrichment of cDNAs encoding ion transport proteins in the subtracted library was not surprising since ion transport is a critical function in the HMT tissues, and the Malpighian tubule epithelia is known to have numerous ion transporting ATPases on both the basolateral and apical membranes (Blum, 1985; Caruso‐Neves & Lopes, 2000). The enrichment of cDNAs encoding vesicle‐associated proteins in the subtracted library is consistent with the fact that HMT tissues are essentially secretory in nature and thus have high levels of cytoplasmic vesicle trafficking (Blum, 1985).…”

Section: Resultsmentioning

confidence: 99%

“…Ion channels also may be involved directly or indirectly in the reabsorption of salts and nutrients prior to urine excretion. Some of the ion channels known to be involved in these processes include the sodium /potassium ATPase, inward rectifying potassium channel, vacuolar ATPases, sodium/ hydrogen exchangers, sodium channels and chloride channels (Clements, 1992;Caruso-Neves & Lopes, 2000). As can be seen in Table 3, ESTs encoding ion channels of similar or identical functions to these have been identified.…”

Section: Cdnas Encoding Ion Channel Proteinsmentioning

confidence: 99%

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Analysis of expressed sequence tags from subtracted and unsubtracted Ctenocephalides felis hindgut and Malpighian tubule cDNA libraries

Gaines

Brandt

Eisele

et al. 2002

Insect Molecular Biology

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Insect hindgut and Malpighian tubule (HMT) tissues regulate the contents of the haemolymph through the excretion of waste products and the specific reabsorption of nutrients. As such, they perform a role that is essential for survival and may contain molecular targets for insect control strategies. In order to discover genes expressed in the HMT tissues of the cat flea, Ctenocephalides felis, expressed sequence tags (ESTs) were generated from an unsubtracted HMT cDNA library and from a subtracted HMT cDNA library that had been enriched for HMT-specific cDNAs. A total of 4844 ESTs were analysed from both libraries: 3657 from the subtracted library and 1187 from the unsubtracted library. Of the 1418 distinct ESTs identified from both libraries, 953 had significant similarity to other sequences reported in the GenBank database. A comparison of the results from the two libraries confirmed that the percentages of genes likely to be involved with metabolism, cell structure, and digestion were reduced by the subtraction procedure, whereas genes likely to be involved with ion transport were enriched. Analysis of the prevalence of three individual cDNAs in each library revealed that the actin cDNA was reduced in the subtracted library whereas the cDNAs encoding allantoinase and a peritrophin-like protein were greatly enriched in the subtracted library. Northern blot analysis demonstrated that the actin cDNA was expressed in both the HMT and carcass tissues, whereas the allantoinase and peritrophin-like cDNAs were detected exclusively in the HMT tissues. In total, 97 distinct ESTs that appear to encode proteins involved with ion transport were analysed. Some of these proteins may be directly involved with diuresis or the specific reabsorption of salts and nutrients, and thus may be potential molecular targets for flea control strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Cdnas Encoding Ion Channel Proteinsmentioning

confidence: 99%

Analysis of expressed sequence tags from subtracted and unsubtracted Ctenocephalides felis hindgut and Malpighian tubule cDNA libraries

Gaines

Brandt

Eisele

et al. 2002

Insect Molecular Biology

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“…NKA activity in the Malpighian tubules appears to be antidiuretic; in Rhodnius, ouabain (an inhibitor of NKA) stimulates transport of Na + and fluid to the Malpighian tubule lumen (Maddrell and Overton, 1988), and the diuretic hormone 5-HT inhibits NKA activity (Grieco and Lopes, 1997). It is proposed that NKA inhibition leads to the accumulation of intracellular Na + , favoring transport of Na + and water to the lumen (Caruso-Neves and Lopes, 2000). Increased NKA activity in the Malpighian tubules of cold-acclimated crickets could therefore account in part for the decreased primary urine production rate.…”

Section: Cold Acclimation Reduces Fluid Excretion Rates Of the Malpigmentioning

confidence: 99%

The effect of cold acclimation on active ion transport in cricket ionoregulatory tissues

Marteaux

Khazraeenia

Yerushalmi

et al. 2018

Comparative Biochemistry and Physiology Part A: Molecular &

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Cold-acclimated insects defend ion and water transport function during cold exposure. We hypothesized that this is achieved via enhanced active transport. The Malpighian tubules and rectum are likely targets for such transport modifications, and recent transcriptomic studies indicate shifts in Na-K ATPase (NKA) and V-ATPase expression in these tissues following cold acclimation. Here we quantify the effect of cold acclimation (one week at 12°C) on active transport in the ionoregulatory organs of adult Gryllus pennsylvanicus field crickets. We compared primary urine production of warm- and cold-acclimated crickets in excised Malpighian tubules via Ramsay assay at a range of temperatures between 4 and 25°C. We then compared NKA and V-ATPase activities in Malpighian tubule and rectal homogenates from warm- and cold-acclimated crickets via NADH-linked photometric assays. Malpighian tubules of cold-acclimated crickets excreted fluid at lower rates at all temperatures compared to warm-acclimated crickets. This reduction in Malpighian tubule excretion rates may be attributed to increased NKA activity that we observed for cold-acclimated crickets, but V-ATPase activity was unchanged. Cold acclimation had no effect on rectal NKA activity at either 21°C or 6°C, and did not modify rectal V-ATPase activity. Our results suggest that an overall reduction, rather than enhancement of active transport in the Malpighian tubules allows crickets to maintain hemolymph water balance during cold exposure, and increased Malpighian tubule NKA activity may help to defend and/or re-establish ion homeostasis.

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“…gills [173]; freshwater mussel ( Anodonta cygnea ) gills [77]; rainbow trout ( Oncorhynchus mykiss Walbaum ) gills [174]; rabbit cardiac sarcolemma [18]; malpighian tubules from Rhodnius prolixus [24, 25]; Trypanosoma cruzi epimastigotes [21, 145]; cultured MDCK I cells [39]; Entamoeba histolytica [38]; Leshmania amazonensis [36]; and pig kidney [79]. Recently, the Na + -ATPase activity has been reported in homogenates of several rat tissues [136].…”

Section: Identification Of the Ouabain-insensitive Na+-atpase In Diffmentioning

confidence: 99%

The second sodium pump: from the function to the gene

Rocafull

Thomas

Castillo

2012

Pflugers Arch - Eur J Physiol

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Transepithelial Na+ transport is mediated by passive Na+ entry across the luminal membrane and exit through the basolateral membrane by two active mechanisms: the Na+/K+ pump and the second sodium pump. These processes are associated with the ouabain-sensitive Na+/K+-ATPase and the ouabain-insensitive, furosemide-inhibitable Na+-ATPase, respectively. Over the last 40 years, the second sodium pump has not been successfully associated with any particular membrane protein. Recently, however, purification and cloning of intestinal α-subunit of the Na+-ATPase from guinea pig allowed us to define it as a unique biochemical and molecular entity. The Na+- and Na+/K+-ATPase genes are at the same locus, atp1a1, but have independent promoters and some different exons. Herein, we spotlight the functional characteristics of the second sodium pump, and the associated Na+-ATPase, in the context of its role in transepithelial transport and its response to a variety of physiological and pathophysiological conditions. Identification of the Na+-ATPase gene (atna) allowed us, using a bioinformatics approach, to explore the tertiary structure of the protein in relation to other P-type ATPases and to predict regulatory sites in the promoter region. Potential regulatory sites linked to inflammation and cellular stress were identified in the atna gene. In addition, a human atna ortholog was recognized. Finally, experimental data obtained using spontaneously hypertensive rats suggest that the Na+-ATPase could play a role in the pathogenesis of essential hypertension. Thus, the participation of the second sodium pump in transepithelial Na+ transport and cellular Na+ homeostasis leads us to reconsider its role in health and disease.

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Sodium pumps in the Malpighian tubule of Rhodnius sp.

Cited by 12 publications

References 27 publications

Analysis of expressed sequence tags from subtracted and unsubtracted Ctenocephalides felis hindgut and Malpighian tubule cDNA libraries

Analysis of expressed sequence tags from subtracted and unsubtracted Ctenocephalides felis hindgut and Malpighian tubule cDNA libraries

The effect of cold acclimation on active ion transport in cricket ionoregulatory tissues

The second sodium pump: from the function to the gene

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