Susceptibility of cell lines to avian viruses

Simoni, Isabela Cristina; Fernandes, Maria Judite Bittencourt; Custódio, R. M.; Madeira, Alda Maria Backx Noronha; Arns, Clarice Weis

doi:10.1590/s0001-37141999000400015

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…15 On the other hand, detection of IBD virus titer inoculated on Vero cell was not observed and this could be related to the strain of the IBD virus or passage level used. 23 The current study result also indicated presence of difference in time of CPEs observation with that of Silva et al that reported IBDV CPEs in Vero cells at 18 hrs postinfection. 24 The titer of Vero cell adapted IBDV LC-75 strain increased with passage number starting from passage 3 indicating that the vaccine strain is well adapted to Vero cell environment and the virus replicates successfully to high titer as shown in Figure 2.…”

Section: Discussionsupporting

confidence: 86%

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Kebede

Bitew²,

Bari

et al. 2021

VMRR

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Introduction Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease has been endemic in Ethiopia since 2002, and vaccination has been practiced as the major means of disease prevention and control. An IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell, which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells and to evaluate the safety, immunogenicity and protection level. Methods Identity of the vaccine seed was confirmed with gene-specific primers using reverse transcription polymerase chain reaction (RT-PCR). Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage 10. A characteristic virus-induced cytopathic effect (CPE) was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. The virus-induced specific antibody was determined using indirect ELISA after vaccination of chicks through ocular route. Results The antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine, hence no significant difference. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality. Conclusion It is concluded that the IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibody development and successfully protects chicks against challenge with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture at the industrial scale to conquer the limitations caused by using CEF cells and thus to vaccinate chicks population to protect against the circulating IBDV infection.

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Section: Discussionsupporting

confidence: 86%

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Kebede

Bitew²,

Bari

et al. 2021

VMRR

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“…Thawed tissues were homogenized and a 20% suspension with an antibiotic solution (penicillin, streptomycin, mycostatin and gentamycin) was prepared. The supernatant from the suspension was inoculated on confluent monolayers of baby hamster kidney (BHK-21) cells (Simoni et al, 1999;Manvell et al, 2004) in 96-well polystyrene microtitre plates and was examined daily for cytopathic effect (CPE) by light microscopy. The supernatant of the tissue homogenates from the different birds, and from different reovirus strains as control, were inoculated onto confluent BHK-21 cells grown in micro-titre plates.…”

Section: Methodsmentioning

confidence: 99%

Reovirus infections associated with high mortality in psittaciformes in The Netherlands

Brand¹,

Manvell²,

Paul³

et al. 2007

Avian Pathology

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In The Netherlands between January 2002 and December 2004, numerous psittaciformes died showing severe splenomegaly and hepatomegaly with multifocal acute necrosis. At the start of the outbreaks mostly parakeets were affected, but later larger parrots were also involved. Seventy-eight birds showed the same features and six were examined completely, including a virological examination. Tests for polyomavirus, Pacheco's disease (herpesvirus) and circovirus psittacine beak and feather disease (PBFD) viruses and Chlamydophila psittaci were carried out. All results were negative, except for two cases of circovirus infection. Many concurrent bacterial and parasitic infections were seen. Immunohistochemistry revealed reovirus antigen in intralesional mononuclear cells, and reovirus-like particles could be observed by negative contrast electron microscopy. A reovirus was grown and the isolates reacted with polyclonal reovirus antiserum but did not react with monoclonal antibodies against chicken reovirus. The virus was therefore considered a psittacine reovirus. Because reoviruses were seen consistently, they seemed to be the most probable cause of the outbreaks. Climate, the introduction of new birds and the transportation of birds might be other factors involved in the disease seen in The Netherlands. No regional influence could be seen; therefore, we suggested that the virus might be widespread and carriers could be a source of re-introduction.

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“…Successful adaptation of IBDV isolates in chicken embryo fibroblast (CEF), chicken embryo kidneys, and chicken embryo bursas was reported [ 6 , 7 ], but the cells being of primary avian origin have limited lifespan, produce low virus titer, and may contain extraneous avian viruses that may contaminate vaccines developed using them [ 8 , 9 ]. Many continuous cell lines of mammalian origin were reported to support the growth of IBDV isolates including MA-104 [ 10 ], OK [ 11 ], BGM-70 [ 10 , 12 ], Vero cells [ 10 , 13 – 15 ], and RK-13 [ 14 , 16 ]. These cell lines are easier to maintain and free from avian viral contaminants [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

Lawal

Hair-Bejo

Arshad

et al. 2017

Advances in Virology

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Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route. The EP12 isolate was further adapted and serially propagated in BGM-70 cell line up to 20 passages (P20). Characteristic cytopathic effects (CPEs) were subtly observed at P1 in both isolates 72 hours postinoculation (pi). The CPE became prominent at P5 with cell rounding, cytoplasmic vacuoles, granulation, and detachment from flask starting from day 3 pi, up to 7 days pi with titers of 109.50 TCID50/mL and log109.80 TCID50/mL for UPM0081 and UPM190, respectively. The CPE became subtle at P17 and disappeared by P18 and P19 for UPM0081 and UPM190, respectively. However, the presence of IBDV was confirmed by immunoperoxidase, immunofluorescence, and RT-PCR techniques. Phylogenetic analysis showed that these two isolates were of the vvIBDV. It appears that a single mutation of UPM190 and UPM0081 IBDV isolates at D279N could facilitate vvIBDV strain adaptability in CEE and BGM-70 cultures.

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Susceptibility of cell lines to avian viruses

Cited by 7 publications

References 14 publications

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Reovirus infections associated with high mortality in psittaciformes in The Netherlands

Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

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