Detection and characterization of bacteriocin-producing Lactococcus lactis strains

Moreno, Izildinha; Lerayer, Alda Luiza Santos; Leitão, Mauro Faber de Freitas

doi:10.1590/s0001-37141999000200008

Cited by 18 publications

(26 citation statements)

References 23 publications

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“…Moreno et al (18) reported that 8.4% of 167 strains of a culture collection were able to inhibit all the indicator microorganisms studied and Hastings et al (9) isolated five bacteriocin-producing lactic acid bacteria from 139 cultures.…”

Section: Discussionmentioning

confidence: 99%

Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products

Martinis¹,

Públio²,

Santarosa³

et al. 2001

Braz. J. Microbiol.

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Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocinproducing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented antilisterial activity and a potential application as biopreservatives in meat systems.

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Section: Discussionmentioning

confidence: 99%

Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products

Martinis¹,

Públio²,

Santarosa³

et al. 2001

Braz. J. Microbiol.

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“…Moreno et al (1999) have isolated several strains of lactic acid bacteria from food products as cheese and milk. The antagonistic activity was detected through the well diffusion assay on agar plates (Toro, 2005).…”

Section: Isolation and Purificationmentioning

confidence: 99%

Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives

Parada

Caron

Medeiros

et al. 2007

Braz. arch. biol. technol.

262

170

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“…lactis and cremoris (Moreno et al, 1999;Flórez et al, 2008;Lampkowska et al, 2008). lactis and cremoris (Moreno et al, 1999;Flórez et al, 2008;Lampkowska et al, 2008).…”

Section: Transfer Of the Tet(s) Gene To L Monocytogenesunclassified

Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

Guglielmetti

Korhonen

Heikkinen

et al. 2009

FEMS Microbiology Letters

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The transferability of a large plasmid that harbors a tetracycline resistance gene tet(S), to fish and human pathogens was assessed using electrotransformation and conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients (Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore the tetracycline resistance determinant was transferred via electroporation to L. garvieae. A transformant clone was used as the donor in conjugation experiments with three different L. monocytogenes strains. To our knowledge, this is the first study showing the transfer of an antibiotic resistance plasmid from fish-associated lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not the original host of the tetracycline resistance but experimentally created by electroporation. These results demonstrate that the antibiotic resistance genes in the fish intestinal bacteria have the potential to spread both to fish and human pathogens, posing a risk to aquaculture and consumer safety.

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Detection and characterization of bacteriocin-producing Lactococcus lactis strains

Cited by 18 publications

References 23 publications

Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products

Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products

Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives

Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

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