Screening of basidiomycetes for the production of exopolysaccharide and biomass in submerged culture

Abstract

Fifty-six strains of Basidiomycetes, including native Brazilian fungi isolated from different ecosystems and edible mushrooms, were screened for production of exopolysaccharides and biomass in submerged culture. Agaricus sp. (CCB 280) and Oudemansiella canarii (Jungh.) Hohn (CCB 179) were the highest exopolysaccharide producers (6.01 and 3.54 g dry w./l respectively) after 7 days of incubation. The best producer of biomass was Schizophyllum commune Fr.:Fr. (CCB 473) with 16.68 g dry w./l in 14 days of incubation. When the culture filtrate was submitted to freezing prior to polysaccharide precipitation, a gelatinous fraction was formed.

Screening of Mushrooms for Polysaccharides

“…In the present study, G. lucidum was found to be the best producer of polysaccharides, which is similar to earlier studies (14,19). The structure and pharmacological effect(s) of polysaccharides produced by G. lucidum is being actively studied (13,20,21).…”

“…Mycelial mat was separated from liquid culture medium, washed with distilled water, lyophilized and quantified as dry weight (105ºC to constant weight). Isopropanol was added to the culture filtrate (1:1 v/v) and after 24 h at 4ºC, the precipitated exopolysaccharide was separated by centrifugation 200 xg for 10 min and also quantified as dry weight (14). The dried mycelium was extracted with 85% (v/v) ethanol to eliminate low-molecular components.…”

“…PSK (Krestin) from cultured mycelia of Coriolus versicolor (10), Lentinan from Lentinus edodes (11), Schizophyllum from Schizophyllum commune (12), and polysaccharide produced by the liquid culture of Ganoderma lucidum (13) are some examples. Rosana and valeria reported that different strains of Schizophyllum commune, Pycnoporus sanguinvus and Trametes villosa produce different amounts of biomass and polymer (14). In another report, submerged cultures of strains of Schizophyllum commune demon-strated EPS production (15).…”

Screening of Brazilian basidiomycetes for antimicrobial activity

“…However, over 6000 metabolites were already identified from these imperfect fungi, making it more and more difficult to isolate novel bioactive metabolites from them. With the development of new fermentation and purification technologies, basidiomycetes are again receiving attention as potential sources of new classes of antibiotics (Anke 1989, Maziero et al 1999, Suay et al 2000.…”

Novos compósitos de polianilina e exopolissacarídeos com aplicações em biomateriais: uma revisão

“…Porém outra possibilidade de aplicação destes biopolímeros é a saúde humana. Várias pesquisas sobre polissacarídeos fúngicos têm-se concentrado em aplicá-los como agentes anti-tumorais (MAZIERO et al, 1999). Os polissacarídeos que apresentam esta atividade são todos glucanas intimamente relacionados à estrutura do escleroglucana (YANG; LIAU, 1998).…”