Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

Minussi, Rosana Cristina; Soares-Ramos, Juliana R.L.; Coelho, Jorge Luiz Cavalcante; Silva, Daison Olzany

doi:10.1590/s0001-37141998000400002

Cited by 15 publications

(13 citation statements)

References 10 publications

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“…Alternative carbon sources, such as sugar cane juice, have been assayed with the objective of reducing production costs of microbial PGs and PLs [28]. We investigated the eVect of diVerent carbon sources in PL activity of the recombinant strain 105 ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum

Cardoso

Ribeiro

Teixeira

et al. 2007

J Ind Microbiol Biotechnol

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The pectin lyase (PL) is an industrially important enzyme since it is used for maceration and clarification in the process of fruit juice production in food industries. In order to increase the yields of pectin lyase we cloned the plg1 (pectin lyase 1) from Penicillium griseoroseum gene under the control of the strong constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) and the terminator region of the tryptophan synthetase (trpC) gene from Aspergillus nidulans (plasmid pAN52-Plg1) and transformed this construct into the P. griseoroseum strain PG63. One of the pAN52-Plg1 multi-copy transformants (strain 105) grown in culture medium containing glucose or sugar cane juice showed PL activities of 4,804 or 5,202 U ml(-1) respectively, which represented 57- and 132-fold increases. In addition, the apparent specific activity of PL produced by this strain was much higher than the one observed for a commercial pectinase preparation. Evaluation of the extracellular proteins in the culture supernatant of strain 105 by SDS-PAGE showed the presence of a clear and strong band of approximately 40 kDa that probably corresponds to PL. The enzyme yields reported here demonstrate that the system we developed is able to express pectin lyase at levels comparable to, or exceeding, previously reported data.

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Section: Resultsmentioning

confidence: 99%

Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum

Cardoso

Ribeiro

Teixeira

et al. 2007

J Ind Microbiol Biotechnol

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“…The average diameter of the formed halo was 0.93 cm. However, when we compared the P. macrosporus activity of PG (744.2685 U/mL) with microorganisms that have the ability to secrete large amounts of this protein, such as Penicillium griseoroseum (1600 U/mL), we cannot consider this saprobe a significant PG producer (Minussi et al 1998).…”

Section: Coffee Sciencementioning

confidence: 99%

Phialomyces Macrosporus Reduces Cercospora Coffeicola Survival on Symptomatic Coffee Leaves

Laborde

Botelho

Rodríguez

et al. 2019

C.Sci.

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Saprobe fungi and necrotrophic pathogens share the same niche within crop stubble and the search for fungi non-pathogenic to plants that are able to displace the plant pathogens from its overwintering substrate contributes to the disease management. Brown eye spot (Cercospora coffeicola) is among the most important coffee diseases, it is caused by a necrotrophic pathogen that has decaying leaves as its major source of inoculum. We have screened saprobe fungi for the ability to reduce C. coffeicola sporulation and viability and determined the possible mechanisms involved in the observed biocontrol. A selected saprobe fungus, Phialomyces macrosporus, reduced the pathogen’s viability by 40% both in vitro and in vivo. The fungus acts through antibiosis and competition for nutrients. It produced both volatile and non-volatile compounds that inhibited C. coffeicola growth, sporulation, and viability. It also produced the tissue maceration enzyme (polygalacturonase), which reduces the pathogen both in detached leaves or in planta. The reduction in the fungal viability either by the saprobe fungus or its polygalacturonase-fraction supernatant resulted in the reduction of the disease rate. Therefore, P. macrosporus is a potential microbial agent that can be used in an integrated management of brown eye spot through the reduction of the initial inoculum of the pathogen that survives and builds up in infected leaves.

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“…The thermal stability was lower at higher temperatures, retaining only 7% activity after 2.5 h at 60°C. In spite of this, 40°C is most frequently used in juice processing Minussi et al (1998), evaluating the stability of PMGL from P. griseoroseum observed that the enzyme was stable up to 40°C, but lost all its activity when maintained at 55°C during one hour. Silva et al (2002) evaluated the stability of PMGL produced by P. viridicatum RFC3 and found higher thermal stability at 35ºC for one hour.…”

Section: Partial Characterization Of Pectin Liase (Pmgl) Crude Extractmentioning

confidence: 99%

Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum

Zeni

Gomes

Ambroszini

et al. 2014

Braz. arch. biol. technol.

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Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A PlackettBurman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL after 48 h of cultivation in a medium containing pectin (33.0 g/L), yeast extract (30.0 g/L) and potassium phosphate (2.0 g/L) at 30ºC, with a stirring rate of 180 rpm, initial pH 5.5 and 5x106 spores/mL inoculum size. The kinetic evaluation in terms of substrate consumption demonstrated that the maximum production of PMGL was at 72 h, and 40% of the total organic carbon, 25% of the nitrogen, 88% of the magnesium, 13% of the potassium and 66% of the iron were consumed. The pH remained almost stable during the whole period of production (5.33 to 4.9). The partial characterization of the crude PMGL enzyme extract showed optimal pH and temperature of 5.5 and 37°C, respectively.

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Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

Cited by 15 publications

References 10 publications

Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum

Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum

Phialomyces Macrosporus Reduces Cercospora Coffeicola Survival on Symptomatic Coffee Leaves

Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum

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