Histomorphometric comparison of the corpus cavernosum of rats submitted to euthanasia with ketamine and xylazine or isoflurane

Abstract

Purpose: To compare the penile histoarchitecture of rats euthanized with isoflurane or with ketamine and xylazine. Methods: Fourteen male rats were divided into two groups: ISO, with animals euthanized with isoflurane; and K+X, with animals euthanized with ketamine (150 mg/kg) associated with xylazine (15 mg/kg). Immediately after the death, the penises were dissected, fixed in a 4% buffered formalin solution, and processed for histomorphometric analysis. The surface densities (Sv) of the corpus cavernosum structures (connective tissue, smooth muscle, sinusoidal space, and elastic fibers) were evaluated using Image J software. The distribution of collagen types I and III was qualitatively assessed. Statistical analyses were performed using the Student's t test for data comparison, considering it statistically significant when p < 0.05. Results: Regarding the Sv of connective tissue, smooth muscle and sinusoidal space, there were no differences between animals in both groups. On the other hand, the animals euthanized with the association of ketamine and xylazine showed the Sv of elastic fibers 24.8% higher in relation to animals euthanized with isoflurane. Conclusion: The euthanasia method affected one of the morphological parameters of the rat penises. The choice of euthanasia method must be standardized to reduce bias and to obtain reliable and reproducible results.

Effects of dutasteride and tamsulosin on penile morphology in a rodent model

“…Furthermore, the surface density of connective tissue, smooth muscle fibers, and elastic system fibers are commonly assessed for this purpose ( 19 ). This method has been successfully used to determine the proportions of CC components in various situations ( 8 , 18 , 20 , 28 , 30 ). To the best of our knowledge, this study is the first of its kind to show the cavernosal modifications after tamsulosin, either alone or in combined administration with dutasteride.…”

A combination of generated hydrogen sulfide and nitric oxide activity has a potentiated protectant effect against cisplatin induced nephrotoxicity

“…At the end of the protocol, animals were subjected to a 12-h overnight fast prior to dissection. All the animals were euthanised using a mixture of ketamine and xylazine (10:1) at a dose of (0.2 ml/100 g) [ 20 ]and the kidneys were removed immediately, flushed with 0.9% NaCl and then stored in 10% formalin solution for analysis.…”

Histological and Immunohistochemical analysis of possible ameliorative effect of Ashwagandha on Methotrexate-Induced Oral Mucositis in Albino Rats

“…At the end of 8 th day, rats were sacrificed by anesthesia overdose (ketamine 150 mg/kg b.w and xylazine 15 mg/kg b.w) (18) , then the tongue and cheek mucosa were dissected.…”