Sucralfate enemas reduce the oxidative tissue damage and preserves the contents of E-cadherin and ?-catenin in colonic mucosa without fecal stream

Abstract

Purpose: To evaluate the effects of sucralfate enemas in tissue contents of E-cadherin and β-catenin in an experimental diversion colitis. Methods: Thirty-six male Wistar rats were submitted to a proximal colostomy and a distal mucous fistula. They were allocated into three groups: first group received daily saline enemas (2 mL/day) and the two other groups daily enemas with sucralfate at dosage of 1 or 2 g/kg/day, respectively. Six animals of each group were euthanized after two weeks and six animals after four weeks. The inflammation of the excluded mucosa was evaluated by histological analysis. The oxidative damage was quantified by measurement of malondialdehyde tissue levels. The expression of E-cadherin and β-catenin was identified by immunohistochemistry, and its contents were quantified by computer-assisted image analysis. Results: Sucralfate enemas reduced inflammation in animals subjected to treatment with 2 g/kg/day by four weeks, and the levels of oxidative damage in mucosa without fecal stream irrespective of concentration and time of intervention. E-cadherin and β-catenin content increased in segments without fecal stream in those animals subjected to treatment with sucralfate. Conclusion: Sucralfate reduces the inflammation and oxidative stress and increases the tissue content of E-cadherin and β-catenin in colonic mucosa devoid to the fecal stream.

Efficiency and Mechanism Evaluation of Magnolia officinalis Water Extract in Preventing Gastric Ulcer

“…However, after treating these cells with Magnolia officinalis water extract, ROS generation was inhibited. We also confirmed that sucralfate could decrease ROS production, which was consistent with the findings of Sato et al [ 16 ]. We believe that one of the mucosal protective mechanisms of Magnolia officinalis water extract is related to inhibiting ROS generation.…”

The Use of Sucralfate in Clinical Practice

“…Due to these characteristics, with the action of gastric pH, negative aluminum is released, and it is electrostatically prone to bind to positively charged chemical groups. In the case of its cytoprotective action, the transformed compound binds to proteins, peptides, among others, forming a glycoprotein complex that covers the damaged surface and will provide cytoprotection to that area [5,6] (Figure 2). In the gastroesophageal tract, the formation of this physical barrier protects against acid, pepsin, and bile salts, in addition to increasing mucus production.…”