Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

Rodrigues, Lenize da Silva; Bovolato, Ana Lívia de Carvalho; Silva, Bárbara Esteves; Chizzolini, Leticia Victória; Cruz, Bianca Latance da; Moraes, Marcelo Padovani de Toledo; Lourenção, Pedro Luiz Toledo de Arruda; Bertanha, Matheus

doi:10.1590/acb361001

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…The most suitable scaffolds for tissue engineering of blood vessels should be able to withstand vascular stress without degeneration, do not promote thrombotic events, present good interaction with adjacent tissues and implanted cells, and enable nutrient transport through the porosity of their wall until they are fully developed [ 20 ]. More than one of our previous studies [ 15 ] have determined that the method used in the production of a biological scaffold produced by the decellularization of the vena cava in the rabbit model meets these requirements, and the current study demonstrates that the interaction with autologous ASCs promotes a favorable environment, generating the ideal adaptive conditions when it is implanted on the animal’s back.…”

Section: Discussionmentioning

confidence: 75%

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Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

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Peripheral arterial disease (PAD) is the main cause of mortality in the western population and requires surgical intervention with the use of vascular substitutes, such as autologous veins or Dacron or PTFE prostheses. When this is not possible, it progresses to limb amputation. For cases where there is no autologous vascular substitute, tissue engineering with the production of neovessels may be a promising option. Previous experimental studies have shown in vitro that rabbit vena cava can be decellularized and serve as a scaffold for receiving mesenchymal stem cells (MSC), with subsequent differentiation into endothelial cells. The current study aimed to evaluate the behavior of a 3D product structure based on decellularized rabbit inferior vena cava (IVC) scaffolds seeded with adipose-tissue-derived stem cells (ASCs) and implanted in rabbits dorsally subcutaneously. We evaluated the induction of the inflammatory response in the animal. We found that stem cells were positive in reducing the inflammatory response induced by the decellularized scaffolds.

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Section: Discussionmentioning

confidence: 75%

“…Decellularization was carried out using the protocol with 1% SDS for 2 h under shaking in a Shaker (News Brunswick Scientific ® Edison, NJ, USA), at 37 °C. ( Supplementary file S1 ) [ 9 , 13 , 15 ]. The fragments were stored in a refrigerator at 4 °C in a sterile solution containing antibiotics and fungicide.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

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“…Furthermore, the physicochemical signals remain intact for regulating cell adhesion, proliferation, and differentiation, thus, providing a superior substrate for MSC seeding [ 7 ]. The scaffold produced through venous decellularization has biomechanical properties sufficient to resist the stresses of the urinary system and offers an extracellular matrix favorable to the cell population [ 12 , 13 , 14 ]. Tissue engineering research for the production and replacement of urinary bladder tissue has grown in recent decades; however, the clinical applications have not been completely established [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits

et al. 2022

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Background: To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces. Methods: New Zealand rabbits (n = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm2) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm2) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scanning electron microscopy (SEM). Results: The integrity of the urinary bladder was maintained in both groups. A superior regenerative process was observed in the G2 group, compared to the G1 group. We observed a greater urothelial epithelialization and maturity of the mucosa and submucosa fibroblasts. Furthermore, SEM demonstrated a notable amount of urothelial villus in the G2 group. Conclusion: Decellularized vena cava scaffolds were able to maintain the integrity of the urinary bladder in the proposed model. In addition, ASCs accelerated the regenerative process development, observed primarily by the new urothelial epithelization and the maturity of mucosa and submucosa fibroblasts.

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Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

Cited by 2 publications

References 26 publications

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits

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