Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant

Plessis, H.J. Du; Nikolova, R.V.; Egan, B.; Kleynhans, R.

doi:10.1590/2447-536x.v27i3.2353

Cited by 3 publications

(2 citation statements)

References 22 publications

(46 reference statements)

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“…Zat pengatur tumbuh BAP dapat mendukung pertumbuhan tunas aksilar pada tanaman dengan menekan dominansi apikal yang disebabkan oleh aktivitas auksin endogen dalam eksplan (Satriawan et al, 2021). Diantara berbagai jenis sitokinin, BAP dirasa merupakan sitokinin yang paling sering digunakan dan efektif dalam menginduksi tunas aksilar (du Plessis et al, 2021;Satriawan et al, 2021). Mukherjee et al (2018), menyebutkan bahwa diantara dua jenis sitokinin yaitu kinetin dan BA, yang lebih responsif dalam pemunculan dan multiplikasi tunas adalah BA.…”

Section: Pendahuluanunclassified

Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal Wonosobo secara in vitro

Nuraini

Aprilia²,

Murgayanti³

et al. 2022

kultivasi

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AbstrakSaat ini, perbanyakan rami menggunakan rizoma sebagai bahan tanamnya, tetapi dalam produksinya membutuhkan waktu yang lama dan sebagai bahan tanam umur simpannya singkat. Kultur jaringan merupakan salah satu teknologi untuk mendapatkan bahan tanam yang seragam dan sehat dalam waktu yang singkat. Tujuan dilakukannya penelitian ini adalah untuk mengetahui pengaruh konsentrasi BAP terhadap pertumbuhan tunas aksilar pada rami klon lokal Wonosobo dan konsentrasi mana yang memberikan pengaruh terbaik. Percobaan dilakukan di Laboratorium Kultur Jaringan, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Nodus batang dari rami klon lokal Wonosobo diklturkan pada media MS dengan penambahan berbagai konsentrasi BAP selama 8 minggu dan diamati pertumbuhan dan perkembangannya. Desain penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 6 perlakuan yang diulang sebanyak tiga kali. Adapun perlakuannya adalah kontrol (tanpa penambahan BAP), BAP 0,5 mg/L, BAP 1,0 mg/L, BAP 1,5 mg/L, BAP 2,0 mg/L, dan BAP 3,0 mg/L. Hasil percobaan menunjukkan penambahan sitokinin berpengaruh terhadap pertumbuhan tunas aksilar rami klon lokal Wonosobo. Penambahan 0,5 mg/L berpotensi memberikan pengaruh terbaik terhadap pertumbuhan tunas aksilar rami klon lokal Wonosobo dilihat dari waktu muncul tunas, jumlah tunas, jumlah daun, dan tinggi plantlet. Kata Kunci: Benzylaminopurine (BAP) ∙ Rami ∙ Tunas aksilar AbstractCurrently, ramie propagation used the rhizome as the planting material. However, it took long time to produce and the longevity of rhizome as planting material is short. Therefore, another technology approach is needed. Tissue culture is one of alternative technologies that could to produce uniform and healthy planting material within short time. The objective of this research was to determine the effect of BAP concentration on the axillary bud growth of rami local clone of Wonosobo and also determine which concentration gives the best effect. The research was conducted at The Tissue Culture Laboratory of Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. Nodes of ramie local clone of Wonosobo were cultured for 8 weeks on Murashige & Skoog (MS) medium added with various concentrations of BAP and then observed growth and development. The research design was Completely Randomize Design (RCD) with 6 treatments in terms of BAP concentrations and 3 replications. The treatment was ,control (without BAP); BAP 0,5 mg/L, BAP 1,0 mg/L, BAP 1,5 mg/L, BAP 2,0 mg/L, and BAP 3,0 mg/L. The result showed that there was different effect on axillary bud growth. The treatment of MS medium added with 0,5 mg/L BAP potentially showed as the best effect for bud appearance, number of shoots, number of leaves, and plantlet’s length.Keywords: Benzylaminopurine (BAP) ∙ Ramie ∙ Axillary bud

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Section: Pendahuluanunclassified

Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal Wonosobo secara in vitro

Nuraini

Aprilia²,

Murgayanti³

et al. 2022

kultivasi

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“…ex Roxb) Koehne (Dogan, 2018), Hibiscus coddii subsp. barnardii (Plessis et al, 2021), Echinacea angustifolia (Tyub et al, 2021), Anubias heterophylla (Rittirat et al, 2021), Staurogyne repens (Nees) Kuntze (Kose et al, 2021), Campomanesia phaea (Demétrio et al, 2021) and Lindernia antipoda (L.) Alston (Viella, 2021) by tissue culture methods.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Doğan

2022

Natural and Engineering Sciences

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Pogostemon erectus (Dalzell) Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog (MS) food solutions including Zeatin (ZEA: 0.1-2.4 mg/L) and indole acetic acid (IAA: 1.2 mg/L). In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.

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Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

Mumivand

Akter

et al. 2022

Plants

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Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to increase their commercial value, respectively. In vitro cultures are a suitable solution to meet expectations using callus culture, somatic embryogenesis, protoplast culture, and the organogenesis of protocorm-like bodies; many of these techniques are commercially practiced. Factors such as culture media, explants, carbohydrates, plant growth regulators, and light are associated with the success of in vitro propagation. Techniques, especially embryo rescue and somatic hybridization, are widely used to improve ornamentals. The development of synthetic seed allows season-independent seed production and preservation in the long term. Despite the advantages of propagation and the improvement of ornamentals, many barriers still need to be resolved. In contrast to propagation and crop developmental studies, there is also a high scope for molecular studies, especially epigenetic changes caused by plant tissue culture of ornamentals. In this review, we have accumulated and discussed an overall update on cultivation factors, propagation techniques in ornamental plant tissue culture, in vitro plant improvement techniques, and future perspectives.

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Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant

Cited by 3 publications

References 22 publications

Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal Wonosobo secara in vitro

Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal Wonosobo secara in vitro

In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application

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