AgNO3 improved micropropagation and stimulate in vitro flowering of rose (Rosa x hybrida) cv. Sena

Abstract

Rose is one of the most important cut flower in the world. Rose micropropagation was used for production of clonal and disease-free plantlets and to breeding purposes. However, many important rose cultivars showed physiological disorders as early-leaf senescence and very low multiplication rate under in vitro conditions. Our hypothesis is that these symptoms were associated with high sensibility of these cultivars to ethylene accumulation on in vitro environment. The rose cv. Sena was in vitro cultivated under different concentrations of AgNO3 and two light sources, LED and fluorescent lamps, as a way to investigate in vitro similar symptoms to ethylene accumulation. AgNO3 at 1.0-2.0 mg L-1 solved the main in vitro physiological disorders observed in this rose cultivar. Also, AgNO3 stimulated induction of 50% of rose shoots to in vitro flowering at 2.0 mg L-1. Higher concentrations also resulted in flowering induction, but with imperfect flower development.

Exploring the connection between ethylene and somatic embryogenesis: an in silico analysis of gene pathways

“…The superabundant presence of ethylene, an endogenous plant hormone, within in vitro cultures can give rise to adverse physiological repercussions, particularly in species or genotypes that exhibit heightened sensitivity, thereby jeopardizing the quality of micropropagated seedlings. Within in vitro cultivation environments, the accumulation of ethylene within containers is frequently correlated with deleterious consequences, including impediments to shoot formation, curtailment of tissue regeneration, and reduction in the occurrence of adventitious shoot formation in certain species (Juras et al, 2020;Matos et al, 2020).…”