The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions

Yurievna, Nabieva Alexandra; Vladimirovna, Gerasimovich Lyudmila

doi:10.1590/2447-536x.v25i4.2035

Cited by 5 publications

(1 citation statement)

References 22 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in the present study, the strong morphogenetic effect of TDZ was also observed in our earlier studies in which TDZ combined with NAA was essential for the cyclic multiplication of tulip adventitious shoots [11]. A similar PGR combination was used to induce the direct formation of adventitious shoots from vegetative buds isolated from uncooled bulbs of tulip 'Apeldoorn' [35] and generative seedlings of Tulipa kaufmanniana [36]. These correspond to numerous reports, which showed that TDZ, a derivative of diphenylurea with cytokinin-like activity, induces morphogenetic processes much more strongly than purine cytokinins [37,38].…”

Section: Se Induction and Embryo Developmentsupporting

confidence: 84%

Micropropagation of Tulip via Somatic Embryogenesis

Podwyszyńska

Marasek-Ciołakowska

2020

Agronomy

View full text Add to dashboard Cite

An effective method of tulip regeneration via somatic embryogenesis (SE) was developed. Explants, flower stem slices excised from cooled bulbs were incubated in darkness on MS modified media containing auxins alone (2,4-dichlorophenoxyacetic acid—2,4-D, 1-naphthalene acetic acid—NAA and 4-amino-3,5,6-trichloro-2-pyridine carboxylic acid—picloram) or combined with thidiazuron (TDZ) at 0.1 and 0.5 mg L−1. Yellowish-white callus with a granular structure was developed in the presence of all auxins on the cut surface from the tissues of the vascular bundles. From this, lines of embryogenic calli were derived. The addition of TDZ to the medium with auxins significantly stimulated somatic embryo formation. Cyclic and the most intensive proliferation of embryogenic callus as well as embryo formation was obtained in the presence of 2,4-D at 0.1 mg L−1 combined with TDZ at 0.5 mg L−1. Addition of proline enhanced either callus proliferation rate or frequency of embryo formation. The best quality embryos with cotyledons longer than 10 mm able to form bulbs were recorded when TDZ was replaced with 6-benzylaminopurine (BAP) at the concentration of 0.1 mg L−1. Histomorphology showed that the development of somatic embryos could have either external or internal origins. Embryos of external origin were initiated by cell division on the edge of embryogenic calli. Embryos of internal origin resulted from the division of parenchyma cells inside the tissue. Embryonic cells were characterized by their small volume, regular shape, dense cytoplasm and large nuclei. The globular embryos were covered by a distinct layer of periderm. Then, the embryos developed into structures having leaf-shaped cotyledons with a procambial strand and a sideward-orientated meristem of the vegetative apex (stolon). Cotyledon embryos did not show vascular connections with the parent tissue, and they did not develop embryonic roots.

show abstract

Section: Se Induction and Embryo Developmentsupporting

confidence: 84%

Micropropagation of Tulip via Somatic Embryogenesis

Podwyszyńska

Marasek-Ciołakowska

2020

Agronomy

View full text Add to dashboard Cite

show abstract

Cold treatment promotes in vitro germination of two endangered Lachenalia species

Maslanka

Kapczyńska

2021

South African Journal of Botany

View full text Add to dashboard Cite

Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

et al. 2021

View full text Add to dashboard Cite

In vitro culture of twin-scaling explants of Galanthus transcaucasicus with different concentrations of plant growth regulators (PGRs) including 0.5, 1, 2, 3, 4, 6, 8, and 10 mg L-1 naphthaleneacetic acid (NAA) and 0.5, 1, 2, 3, and 4 mg L-1 benzyladenine (BA) was studied. After 18 weeks, the number of regenerated bulblets and intensity of callus was measured. Subsequently, bulblets were transferred to a medium with 0.5, 1, 2, 3, and 4 mg L-1 NAA and 0.5, 1, 2, 3, and 4 mg L-1 BA and, after 15 weeks, the bulblets length and diameter were measured. The highest intensity of callus was obtained on 4 mg L-1 NAA or 8 mg L-1 NAA with 1 mg L-1 BA. The highest number of regenerated bulblets was detected with 6 mg L-1 NAA and 2 mg L-1 BA. The highest diameter of bulblets occurred on four mgL-1 NAA (9.4 mm), while the lowest was observed on 0.5 mg L-1 BA (1.83 mm). The analysis of genetic variation using ISSR revealed that there was no somaclonal variation among the regenerated plants from BA and low level of NAA, but there was a significant somaclonal variation at high concentrations of NAA.

show abstract

The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions

Abstract: The applicaTion of differenT reproducTion Techniques for rare species waTerlily Tulip (Tulipa kaufmanniana regel.) propagaTion under ex siTu condiTions 450

Cited by 5 publications

References 22 publications

Micropropagation of Tulip via Somatic Embryogenesis

Micropropagation of Tulip via Somatic Embryogenesis

Cold treatment promotes in vitro germination of two endangered Lachenalia species

Micropropagation and assessment of somaclonal variation in Galanthus transcaucasicus in vitro plantlets

Contact Info

Product

Resources

About