Six-week anaerobic training improves proteolytic profile of diabetic rats

Abstract

Objective: To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats. Materials and methods: Four groups were established: DE (Diabetes Exercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05. Results: We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level of E2-14kDa gene expression. Conclusion: Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats. Arch Endocrinol Metab. 2015;59(5):400-6

Fisioterapia convencional versus conceito Balance sobre alterações sensório-motoras da neuropatia diabética

“…Nesse âmbito, uma pesquisa sobre exercícios e auto-cuidados nesses pacientes 17 aponta para este fato e corrobora com os achados atuais alegando que as manifestações supracitadas podem levar a maiores complicações, como quedas e risco de amputações nos membros inferiores, uma vez que além dos danos neuropáticos, a fisiopatologia desta doença perpassa ainda por alterações circulatórias. Diante disso, o exercício é reconhecido como uma terapêutica não invasiva, acessível e de baixo custo, capaz de evitar ou mesmo reduzir os danos descritos 18,19 . Diante disso, um estudo 20 apresentou proposta semelhante à deste ao analisar o equilíbrio de diabéticos neuropatas após intervenção fisioterapêutica comparando a terapia convencional com uso de Estimulação elétrica transcutânea (TENS) e infravermelho, ao tratamento sugerido pelos autores, o qual foi somado aos recursos anteriores uma plataforma cujo objetivo destinava-se ao treino de equilíbrio pela manutenção de posturas com estímulo a estratégias motoras.…”