Biometrical and morphological analyses of Macrobrachium olfersii (Wiegmann, 1836) (Crustacea, Decapoda, Palaemonidae) embryos exposed to UVA and UVB radiation

Müller, Yara Maria Rauh; Quadros, Thaline de; Schramm, Heloísa; Weiss, Valquíria Machado Cardoso; Zeni, Eliane Cristina; Nazari, Evelise Maria; Ammar, Dib

doi:10.1590/2358-2936e2018013

Cited by 4 publications

(4 citation statements)

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“…It has been reported that exposure to UVB for 0.5 h (3100 W/m 2 ) induced cell death-associated delay in embryonic development in the Bristled River Shrimp Macrobrachium oldersii. 75 Acute exposure to UVB for 1 h (12 ± 5 W/m 2 , total dose: 0.5−1.5 kJ/m 2 ) caused damage to the skin and muscle cells in earthworms Amynthas gracilis and Metaphire posthuman. 76 The former species showed cuticle breakdown and epidermis, circular muscle and longitudinal muscle necrosis, whereas the latter displayed epidermal cell necrosis and deformed circular muscle.…”

Section: ■ Results and Discussionmentioning

confidence: 98%

“…magna, such as poor organ compartmentalization (Figure C, f), thinner epidermis (Figure C, g), lack of embryonic development (Figure C, h), abnormal thoracopod development (Figure C, i), and malformation of the ovarian structure (Figure C, j) compared to the controls. It has been reported that exposure to UVB for 0.5 h (3100 W/m 2 ) induced cell death-associated delay in embryonic development in the Bristled River Shrimp Macrobrachium oldersii . Acute exposure to UVB for 1 h (12 ± 5 W/m 2 , total dose: 0.5–1.5 kJ/m 2 ) caused damage to the skin and muscle cells in earthworms Amynthas gracilis and Metaphire posthuman .…”

Section: Resultsmentioning

confidence: 99%

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De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Shang

Xie

Lee

et al. 2020

Environ. Sci. Technol.

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Ultraviolet B (UVB) radiation is a natural nonchemical stressor posing potential hazards to organisms such as planktonic crustaceans. The present study was conducted to revisit the lethal effects of UVB on crustaceans, generate new experimental evidence to fill in knowledge gaps, and develop novel quantitative adverse outcome pathways (qAOPs) for UVB. A combination of laboratory and computational approaches was deployed to achieve the goals. For targeted laboratory tests, Daphnia magna was used as a prototype and exposed to a gradient of artificial UVB. Targeted bioassays were used to quantify the effects of UVB at multiple levels of biological organization. A toxicity pathway network was assembled based on the new experimental evidence and previously published data extracted using a novel computational tool, the NIVA Risk Assessment Database (NIVA RAdb). A network of AOPs was developed, and weight of evidence was assessed based on a combination of the current and existing data. In addition, quantitative key event relationships in the AOPs were developed by fitting the D. magna data to predefined models. A complete workflow for assembly and evaluation of qAOPs has been presented, which may serve as a good example for future de novo qAOP development for chemical and nonchemical stressors.

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Section: ■ Results and Discussionmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Shang

Xie

Lee

et al. 2020

Environ. Sci. Technol.

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“…Macrobrachium olfersii (Decapoda: Palaemonidae) has become an emerging model for developmental and toxicological studies (Dos Santos et al, 2020;Jaramillo et al, 2016Jaramillo et al, , 2017Müller et al, 2018;Nazari et al, 2010;Schramm et al, 2017). This species has a wide geographic distribution in the Americas (Rossi & Mantelatto, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

et al. 2022

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Hox genes encode transcription factors that specify the body segment identity during development, including crustaceans, such as amphipods and decapods, that possess a remarkable diversity of segments and specialized appendages. In amphipods, alterations of specialized appendages have been obtained using knockout experiment of Hox genes, which suggests that these genes are involved in the evolution of morphology within crustaceans. However, studies of Hox genes in crustaceans have been limited to a few species. Here, we identified the homeodomain of nine Hox genes: labial (lab), proboscipedia (pb), Deformed (Dfd), Sex combs reduced (Scr), fushi tarazu (ftz), Antennapedia (Antp), Ultrabithorax (Ubx), abdominal‐A (abdA), and Abdominal‐B (AbdB), and evaluated their expression by RT‐qPCR and RT‐PCR in the ovary, during embryonic development, and at the first larval stage (Zoea I) of the decapod Macrobrachium olfersii. The transcript levels of lab, Dfd, and ftz decreased and transcripts of pb, Scr, Antp, Ubx, abdA, and AbdB increased during embryonic development. Hox genes were expressed in mature ovaries and Zoea I larval stages, except Scr and ftz, respectively. In addition, isoforms of Dfd, Scr, Ubx, and abdA, which have been scarcely reported in crustaceans, were described. New partial sequences of 87 Hox genes from other crustaceans were identified from the GenBank database. Our results are interesting for future studies to determine the specific function of Hox genes and their isoforms in the freshwater prawn M. olfersii and to contribute to the understanding of the diversity and evolution of body plans and appendages in Crustaceans.

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“…100 to 400 nm and is not visible to humans. UV radiation is classified as UVA (400-329nm), UVB (320-290nm) or UVC (<290nm) (Diffey, 2002;Grimes, 2015). 90% of the solar UVA radiation reaches the Earth surface.…”

Section: Introductionmentioning

confidence: 99%

UV radiation in HCT 116 cells influences intracellular H2O2 and glutathione levels, antioxidant expression, and protein glutathionylation

Adamiec

Skonieczna

2019

Acta Biochim Pol

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UV radiation influences cellular levels of hydrogen peroxide (H2O2) and glutathione (GSH) and alters the expression of antioxidant genes in the human colorectal cancer cell line HCT116. In this study, cells were irradiated with UV light of different wavelength (A, B, or C). A surge in H2O2 concentration and total glutathione (level occurred 6 hours later. Consequently, protein glutathionylation increased above control levels. Expression of the antioxidant enzymes: glutathione peroxidase (GPX) and glutathione reductase (GSR), assessed by real-time quantitative PCR, increased by 1.5–2 times after 24 hours post-irradiation, in comparison to the untreated controls. Glutathionylation of proteins was enhanced after UV radiation and the set of biotinylated glutathione ethyl ester (BioGEE) tagged proteins was detected by Western Blot procedure. This specific glutathione analogue is conjugated with antioxidant proteins during glutathionylation especially under oxidized conditions in cells. A pool of glutathionylated proteins in the treated cells showed peculiar characteristics. These proteins exhibited varying molecular weights. For UVA-irradiated cells, 24 hours after the treatment we observed two additional ~60 and ~72 kDa bands of glutathionylated proteins from NADPH oxidases (NOX family). Total glutathione level in the UV-irradiated HCT116 cells was higher than in the control. This correlates with the detection of glutathionylation in UV-irradiated cells in the first and twelfth hour of post-irradiation, and can be defined as a specific antioxidant element activation for cellular protection.

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Biometrical and morphological analyses of Macrobrachium olfersii (Wiegmann, 1836) (Crustacea, Decapoda, Palaemonidae) embryos exposed to UVA and UVB radiation

Cited by 4 publications

References 24 publications

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

De Novo Development of a Quantitative Adverse Outcome Pathway (qAOP) Network for Ultraviolet B (UVB) Radiation Using Targeted Laboratory Tests and Automated Data Mining

Identification of Hox genes and their expression profiles during embryonic development of the emerging model organism, Macrobrachium olfersii

UV radiation in HCT 116 cells influences intracellular H2O2 and glutathione levels, antioxidant expression, and protein glutathionylation

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