Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

Abstract

Analyses of germination of seeds under artificial stress conditions are tools for a better understanding of the survival and adaptation capacity of species under natural stress conditions. Therefore, the aim of this study was to evaluate the effect of priming on seed germination, on breaking of dormancy, and on the length of Urochloa brizantha seedlings under water restriction and salt stress conditions. A completely randomized experimental design was used with four replications in a 3 × 6 factorial arrangement consisting of 3 germination conditions (water, water restriction with PEG, and salinity with NaCl) and 6 treatments [five priming solutions (distilled water, potassium nitrate, spermidine, gibberellin, and sodium nitroprusside) and a control (unprimed seeds)]. The following determinations were made: seed moisture content after priming (before drying) and after drying the seeds, germination test at seven and twenty-one days after sowing, germination speed index, viability by the tetrazolium test of the seeds remaining from the germination test, and seedling length. Priming is effective in minimizing the negative effects of water restriction and salinity. Use of sodium nitroprusside as a priming solution results in a lower percentage of dormant seeds and greater germination, vigor, and plant development.

OSMOPRIMING AND ITS RELATIONSHIP WITH THE QUALITY OF Urochloa decumbens SEEDS UNDER WATER DEFICIT

“…SNP stands out for being a donor of nitric oxide (NO), an inorganic radical synthesized from L-arginine (Kolbert et al, 2021), which acts in several cellular processes, stimulating germination and breaking dormancy of several species, such as mustard (Rather et al, 2020), tomato (Khan et al, 2021), and pea (Sekita et al, 2022). Similarly, Oliveira et al (2021) observed positive results in the germination of U. brizantha when primed with SNP, showing the positive role of this compound. In contrast, the variables of germination speed index (GSI) and tetrazolium test (TZ) stand out for the osmopriming carried out with PEG.…”

“…Osmopriming can be performed using different osmotic solutions, such as spermidine (Lopes et al, 2018), gibberellin (Li et al, 2019), sodium nitroprusside (SNP) (Kaiser et al, 2016), polyethylene glycol (PEG-6000) (Lei et al, 2021), and sodium selenate (Silveira et al, 2023). Oliveira et al (2021) evaluated the effect of osmopriming with SNP in U. brizantha seeds and found an improvement in the physiological performance of seeds under water and saline stress conditions.…”

Irrigation with saline water in the cultivation of mini watermelon under phosphate fertilization

“…Salt stress in plants causes various physiological and metabolic changes, such as nutritional imbalance and oxidative stress, which lead to reductions in water absorption and growth, and can also cause toxicity, due to the action of Na + and Clions (Oliveira et al, 2021(Oliveira et al, , 2022Pinheiro et al, 2022). In addition, it alters water relations by inducing stomatal closure and inhibition of leaf expansion.…”

Nitric Oxide in Seed Biology

“…Rich.) R. Webster) seeds with SNP (100 µM) induced protective action against water restriction and salinity [ 52 ]. In some reports, authors confirmed the positive action of NO after using SNP solution by applying a NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxide; cPTIO), an inhibitor of NO synthase (L-N G -nitro arginine methyl ester), an inhibitor of NR (sodium tungstate), or methylene blue, which is regarded as a blocker of NO action [ 42 , 51 , 53 ].…”