Radiographic analysis for the evaluation of polyembryony in Swingle citrumelo seeds

Abstract

The polyembryony rate is a very important factor to consider when choosing a commercial rootstock. Currently, automated systems are used to improve seed quality analyses. X-ray testing is a fast, simple, non-destructive, high-precision test that allows to examine in detail the internal morphology of the seeds to identify damaged areas, their location and types of damage. In this context, the present research aimed to verify the possibility of using X-ray test to evaluate the polyembryony in Swingle citrumelo seeds. Seeds from seven lots were submitted to X-ray tests, direct method (embryo counts) and indirect method (germination). According to the results obtained, it was observed that there was a high coincidence between the number of embryos per seed analyzed using X-ray test and the direct method. Radiographic image analysis is efficient to evaluate the polyembryony in seeds of Swingle citrumelo.

High-throughput phenotyping of brachiaria grass seeds using free access tool for analyzing X-ray images

“…In this respect, the configurations of the X-ray equipment used in this study proved to be adequate and standardizable for future experiments with this equipment. Nevertheless, it is known that different X-ray systems might not produce similar results due to the types of X-ray detectors used Other studies have shown that the information generated by simple visual evaluation of the X-rays are highly valuable for analysis of the physical quality of the seeds, and it is a good tool for monitoring germination (Nielsen et al 2017), selection of more vigorous seed lots (Abud et al 2018, and identification of polyembryony (Arruda et al 2018). In this study, the ability to use the X-ray images to visualy identify tissue deterioration brought about by pathogenic agents at different levels is another promising result, which may contribute to fortification of sanitary barriers, avoiding contamination of crops by infested seeds (Figure 2).…”

“…Among these technologies, the X-ray test has been successfully applied for non-destructive evaluation of indicators that are not visible in seeds (Huang et al 2015, Kotwaliwale et al 2014, Rahman & Cho 2016. Several studies have recommended the use of the X-ray technique for analysis of inner seed morphology, seeking to obtain faster and more accurate diagnoses regarding physical characteristics of interest, often related to seed germination and vigor (Abud et al 2018, Arruda et al 2018, Nielsen et al 2017, Noronha et al 2018.…”

Qualitative and Quantitative Evaluation Protocol of Baccharis Seed Germination

“…The X-ray was performed to identify fully formed (TF), partially formed (FP) and non-formed (NF) seeds (ISTA, 2008). X-ray test was done since is a fast and non-destrutive test to determine morphology seed, check presencing full and empty seeds, mechanical damage and little insects (Arruda et al, 2018).…”

EVALUATION OF HETEROGENEITY AND HIDDEN DEFECTS OF WHEAT (Triticum aestivum L.) SEEDS BY INSTRUMENTAL PHYSICAL METHODS

“…Отметим, что в настоящее время в Российской Федерации доля некондиционных семян зерновых культур может доходить до 40 % (4). Для контроля качества семенного материала существует ряд стандартных тестов, регламентированных ISTA (International Seed Testing Association, Швейцария), а также перспективных инструментальных методов (5)(6)(7)(8).…”