Seed cryopreservation of Passiflora species

Araújo, Deizimary Stella de; Luz, Petterson Baptista da; Neves, Leonarda Grillo; Sobrinho, Severino de Paiva

doi:10.1590/2317-1545v38n3154922

Cited by 11 publications

(7 citation statements)

References 21 publications

(12 reference statements)

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“…Even more, as was observed in three species, cryostorage enhanced germination. Similar response was reported for several orchid seeds (Nikishina et al, 2007;Popov et al, 2004); for Pyrus communis (Reed et al, 2001), Halimium atriplicifolium, Helianthemum apenninum, Helianthemum squamatum (P erez-García and Gonz alez-Benito, 2008), Angelica ursina, Oxytropis chankaenis, Oxytropis retusa (Kholina and Voronkova, 2008), Elaeis guineensis (Camillo et al, 2009), Passiflora suberosa, and Passiflora edulis (Ara ujo et al, 2016); and for wild medicinal legume species (Kholina and Voronkova, 2012) after seed cryostorage. It has been suggested that LN exposure may enhance the germination of treated seeds by breaking seed dormancy or softening of the seed coat (Kholina and Voronkova, 2008;Reed et al, 2001).…”

Section: Discussionsupporting

confidence: 69%

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Dolce¹,

Medina²,

González-Arnao

2018

horts

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This research aimed at evaluating the desiccation tolerance and ability to withstand cryostorage of intact seeds of seven South American Ilex species, and comparing different methodologies for in vitro germination of fresh and cryostored seeds. Intact seeds were silica gel–desiccated from 2 to 14 hours, placed in cryovials, and immersed in liquid nitrogen (LN). Survival was assessed through in vitro germination of intact seeds, bisected seeds, or isolated embryos. Seeds of the seven Ilex species (Ilex brasiliensis (Sprengel) Loes., Ilex brevicuspis R., Ilex dumosa var. dumosa R., Ilex integerrima (Vell. Conc.) R., Ilex paraguariensis A. St. Hil., Ilex pseudoboxus R., and Ilex theezans R.) tolerated desiccation to ≈6% moisture content (MC) and could be successfully cryopreserved when MC decreased between 6.4% and 8.4% depending on the species, before immersion in LN. In addition, it was established as the optimal condition for in vitro seed germination of the seven Ilex species. A simple and cost-effective cryogenic procedure (which did not require the use of cryoprotectants or sophisticated facilities) was defined for seeds of seven Ilex species, which provides a new alternative for safe long-term preservation of Ilex germplasm.

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Section: Discussionsupporting

confidence: 69%

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Dolce¹,

Medina²,

González-Arnao

2018

horts

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“…This was not the case for Anacardium humile because the means were not different from those found when cryopreserving the achenes in LN without cryoprotectant (71%), despite the high percentages of germination found when using cryoprotectant (LN-DMSO). Different results were reported for Passiflora mucronata, P. suberosa and P. edulis seeds, which may be preserved using long-term cryopreservation without cryoprotectants (Araujo et al, 2016). Notably, no decrease in physiological potential was observed in achenes cryopreserved without cryoprotectant, when compared with achenes cryopreserved with cryoprotectant.…”

Section: Discussionmentioning

confidence: 69%

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

Barbosa¹,

Sales²,

Silva³

et al. 2019

Aust J Crop Sci

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The present study aimed to evaluate the possibility of using cryopreservation method for conservation of cajuzinho-do-cerrado achene (Anacardium humile). To accomplish this aim, the physiological quality of cryopreserved achenes was compared with the quality of samples stored under other conditions. The quality of cryopreserved achenes with different water contents was also evaluated to establish a water-content limit for seed-viability maintenance. Achenes with physiological maturity were stored in liquid nitrogen at -196 °C with the following water contents: 28 (initial content) 14, 12, 8 and 5% moisture content (m c ). Subsequently, the achenes were subjected to 3 thawing methods: slow/gradual, fast and microwave thawing. Achenes were also subjected to different six conservation methods: cryopreservation in liquid nitrogen (LN) at -196 °C, cryopreservation using 10% cryoprotectants (Dimethyl sulfoxide (DMSO) or Glycerol), 10 °C biochemical oxygen demand (BOD) incubator, -80 °C ultra-low freezer and room temperature (25 °C ±3), with testing after storage in these environments for 5 and 10 months. The treatment effect on achene, physiological quality was assessed by seed germination (percentage) and vigour (germination speed index, electrical conductivity and root and shoot length). The results showed that reduction in the water content considerably reduced the germinating potential of Anacardium humile achenes for cryopreservation. Conversely, the thawing methods had no effect on the physiological quality of cryopreserved achenes; thereby, allowing the choice of the most practical thawing method (microwave). We also concluded that cryopreservation in liquid nitrogen may be used as a viable alternative for conservation of Anacardium humile achene.

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“…Provavelmente, este alto percentual pode ser atribuído ao conteúdo de água das sementes de 7,5%, considerado um valor seguro para criopreservação da espécie, conforme descrito no trabalho realizado por Veiga- Barbosa et al (2013). Araújo et al (2016) trabalhando com sementes de maracujá com conteúdo de agua nas sementes iguais e inferiores a 12% também obtiveram sucesso na criopreservação das sementes sem o uso de crioprotetores. Segundo González-Benito et al (2009), na criopreservação de sementes de Passiflora é importante que o conteúdo de água das sementes seja mantido entre um valor não muito alto para que não ocorra a formação de cristais de gelo ou muito baixo, o que pode induzir danos ao embrião pela dessecação.…”

Section: Resultsunclassified

Crioprotetores na criopreservação de sementes de Passiflora mucronata Lam

et al. 2019

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RESUMO -O Brasil é um dos centros de diversidade do gênero Passiflora L. e possui um potencial para a utilização dos recursos genéticos na área agronômica. Passiflora mucronata Lam. é uma espécie silvestre com ótimas características para uso ornamental. Este estudo teve por objetivo avaliar o efeito de diferentes concentrações dos crioprotetores: dimetilsulfóxidoe sacarose (0, 7, 14, 21 e 28 %) na criopreservação de sementes de P. mucronata. Após a aplicação dos crioprotetores, as sementes foram armazenadas em nitrogênio líquido durante cinco dias, descongeladas, posteriormente semeadas em vermiculita e mantidas em temperatura de 27,7 ± 1,9°C. Foram avaliadas as variáveis: emergência, índice de velocidade de emergência, comprimento de parte aérea e raiz e massa seca de plântulas. O tipo de crioprotetor e a aplicação de doses crescentes afetam a qualidade fisiológica de sementes após o armazenamento em nitrogênio líquido. As sementes podem ser armazenadas em nitrogênio líquido sem o uso de crioprotetores. Palavras-chave: dimetilsulfóxido, maracujá, ornamental, sacaroseABSTRACT -Cryoprotector on cryopreservation of Passiflora mucronata Lam. Brazil is one of the centers of diversity of the genus Passiflora L. and therefore has great potential for the utilization of the genetic resources in agronomy. Passiflora mucronata Lam. is a wild species that presents great characteristics for ornamental use. This work aimed to evaluate the effect of different concentrations of cryoprotectants dimethylsulfoxide (DMSO) and sucrose (0, 7, 14, 21 and 28%) on the cryopreservation of Passiflora mucronata seeds. After the application of the cryoprotectants, the seeds were stored in liquid nitrogen for 5 days, thawed, and later, seeded in vermiculite and maintained at a temperature of 27.7 ± 1.9°C. The cryoprotectant type and the application of increasing doses affect the physiological quality of seeds after storage in liquid nitrogen. Cryopreservation may be used for the long-term conservation of seeds, without the use of cryoprotectants.

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Seed cryopreservation of Passiflora species

Cited by 11 publications

References 21 publications

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

Crioprotetores na criopreservação de sementes de Passiflora mucronata Lam

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