Tetrazolium test for viability estimation of Eugenia involucrata DC. and Eugenia pyriformis Cambess. seeds

Cripa, Fernanda Bernardo; Freitas, Laura Cristiane Nascimento de; Grings, Andrieli Cristine; Bortolini, Michele Fernanda

doi:10.1590/2317-1545v36n3991

Cited by 11 publications

(12 citation statements)

References 19 publications

(14 reference statements)

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“…The germination test has some limitations on its accomplishment, such as delays and possible modifi cations of the results due to the presence of fungi. Thus, it is necessary to use fast and reliable tests to estimate the viability of storage seeds (Cripa et al, 2014), such as the tetrazolium test, which is based on the activity of the dehydrogenase enzymes involved in the respiration process (Sousa et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

et al. 2019

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Studying the physiological and biochemical behavior of Myrcianthes pungens seeds stored in different packages for up to ten months was the objective of this work. Seeds were collected in Toledo, Pato Bragado, and Marechal Cândido Rondon and stored in a cold and dry chamber (11 ºC and 6,3% RH) for ten months. The germination test and the germination speed index (GSI), the seedling length (SL), the seedling dry matter mass (DMS), and the tetrazolium (ZT) test were performed and, for each evaluation, the water content was determined. Non-parametric analysis was used. During storage, the water content of the seeds in the plastic and glass containers varied little, but decreased in the paper packaging. The germination was maintained for ten months when the seeds were stored in plastic containers and for two months in the glass and paper ones. The variables GSI, SL, and DMS presented upwards and downwards variations during the storage period. The ZT revealed that, in the plastic packaging, the seeds remained viable for up to ten months, in glass, up to two months, and in paper, up to four months. Therefore, seeds of Myrcianthes pungens with high initial quality can be stored in a cold and dry chamber in plastic bags, maintaining viability and vigor for a period of ten months

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Section: Introductionmentioning

confidence: 99%

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

et al. 2019

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“…The 'cerejeira do mato' or 'cerejeira do Rio Grande' (Eugenia involucrata DC) presents predominantly the occurrence in the semideciduous forest and araucaria forests (Sausen et al, 2009). Fruiting occurs from October to December (Cripa et al, 2014). The fruits of this species are used for both 'in natura' consumption and for processing, obtaining sweets and jellies.…”

Section: Introductionmentioning

confidence: 99%

‘Cerejeira da mata’ and ‘guabijuzeiro’ propagation by air layering

et al. 2018

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‘Guabijuzeiro’ and ‘cerejeira da mata’ are plant species from the Myrtaceae family, with many difficulties in asexual multiplication. Thus, the aim of this study was to evaluate ‘cerejeira da mata’ (Eugenia involucrata DC.) tree and ‘guabijuzeiro’ [Myrcianthes pungens (Berg) Legrand] tree propagation by air layering, using different IBA concentrations (0, 1000 , 2000 and 3000 mg L-1) and materials to wrap the substrate (transparent plastic, black plastic and transparent plastic + aluminum foil). The experimental design for both experiments was a randomized blocks, in a 3 x 4 factorial (wrapping material x IBA concentration), with three repetitions of five air layering each. After 180 days, the percentage of rooting, length and number of roots were evaluated. Sixty days after rooting the percentage of survival plants were evaluated. The air layering technique was not efficient in the ‘guabijuzeiro’ propagation. This technique could be used in ‘cerejeira da mata’ plants without the IBA application and using transparent plastic, but with low performance.

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“…Sandwith; Cripa et al (2014), Kaiser et al (2014) and Lamarca and Barbedo (2014) on species of the genus Eugenia; and Nogueira et al (2014) on Pacara Earpod Tree (Enterolobium contortisiliquum (Vell.) Morong).…”

Section: Introductionmentioning

confidence: 99%

Viability of Simira gardneriana M.R. Barbosa & Peixoto seeds by the tetrazolium test

2016

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-Ecoregion Caatinga presents a great diversity of species with potential for exploitation. Among them 'pereirovermelho' (Simira gardneriana M.R. Barbosa & Peixoto) stands out for its importance in timber and forestry activities. Its seeds germinate slowly. Therefore, the use of tetrazolium test to estimate viability becomes essential when quick answers on seeds quality are wanted. This study has aimed to establish the best concentration of tetrazolium solution and the coloration period for assessing the viability of Simira gardneriana seeds. Initially, seeds were subjected to pre-wetting between paper sheets for 144 hours at 30 °C. Subsequently, the endosperm portion containing the embryo was immersed at four concentrations of tetrazolium solution (0.075, 0.1, 0.5 and 1.0%) and three coloration periods (2, 4 and 6 hours) in the dark under the temperature of 30 °C and another one at 35 °C. The percentage of viable seeds was compared with the results obtained in the germination test conducted on paper substrate at 30 °C in four replicates of 25 seeds. Tetrazolium test was efficient to estimate the viability of S. gardneriana seed and the concentration of 0.075% for six hours at 35 °C was the best condition.Index terms: Rubiaceae, forest seeds, caatinga, germination, conservation.Viabilidade de sementes Simira gardneriana M.R.Barbosa & Peixoto pelo teste de tetrazólio RESUMO -A Caatinga apresenta grande diversidade de espécies com potencial de exploração, dentre estas o pereirovermelho (Simira gardneriana M.R. Barbosa & Peixoto) destaca-se pela importância nas atividades madeireira e florestal. Suas sementes germinam lentamente, com isso o uso do teste de tetrazólio para estimar a viabilidade passa a ser essencial quando se deseja respostas rápidas sobre a qualidade das sementes. Assim, objetivou-se com esse trabalho estabelecer a melhor concentração da solução de tetrazólio e o período de coloração para a avaliação da viabilidade de sementes de S. gardneriana. Inicialmente, as sementes foram submetidas ao pré-umedecimento entre papel por 144 horas a 30 °C. Posteriormente, a porção do endosperma contendo o embrião foi imersa em quatro concentrações da solução de tetrazólio (0,075; 0,1; 0,5 e 1,0%) e três períodos de coloração (2, 4 e 6 horas), no escuro, sob a temperatura de 30 °C e outra a 35 °C. A porcentagem de sementes viáveis foi comparada com os resultados obtidos no teste de germinação, conduzido em substrato papel, a 30 °C, em quatro repetições de 25 sementes. O teste de tetrazólio foi eficiente para estimar a viabilidade de sementes de S. gardneriana, sendo a concentração de 0,075% por seis horas, a 35 °C a melhor condição.Termos para indexação: Rubiaceae, sementes florestais, caatinga, germinação, conservação.

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Tetrazolium test for viability estimation of Eugenia involucrata DC. and Eugenia pyriformis Cambess. seeds

Cited by 11 publications

References 19 publications

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

‘Cerejeira da mata’ and ‘guabijuzeiro’ propagation by air layering

Viability of Simira gardneriana M.R. Barbosa & Peixoto seeds by the tetrazolium test

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