Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

Kaiser, Daiana Karoline; Freitas, Laura Cristiane Nascimento de; Biron, Rubia Priscila; Simonato, Simone Cristina; Bortolini, Michele Fernanda

doi:10.1590/2317-1545v36n31022

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…E. pyriformis and E. uniflora were, on average, only 10-20 cm in height one year after sowing (Table 3), which can reduce their chances of establishment in the long term. In addition, their seeds are recalcitrant with high water content when dispersed, which may compromise the seeds' viability in storage conditions (Kaiser et al, 2014) and their use in direct seeding systems. On the other hand, P. elegans seemed to be more suitable for direct seeding systems.…”

Section: Group 1 -Suitable Speciesmentioning

confidence: 99%

“…Other limitations for recommending direct seeding systems used on larger scales include the low seed availability in local markets, both in quantity and quality required (Douglas et al, 2007). Furthermore, most tropical forest species have recalcitrant seeds, which have low seed longevity and lose viability quickly when stored (Fonseca and Freire, 2003;Kaiser et al, 2014). As a consequence, commercial nurseries prefer to sell seedlings instead of seeds.…”

Section: Technical Feasibilitymentioning

confidence: 99%

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Direct seeding reduces costs, but it is not promising for restoring tropical seasonal forests

Souza

Engel

2018

Ecological Engineering

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A B S T R A C TDirect seeding is a potential technique to restore forests; however, further studies are needed before its application on a large scale. We carried out a field experiment in a deforested area in southern Brazil to test the technical and economic feasibility of a direct seeding system with high tree species diversity to restore the tropical seasonal forest. We also compared species performances and tested the effects of seed size and successional group on tree seedling emergence and development. The trial was established at two different sowing times using 31 tree species. For two years after sowing we evaluated seedling emergence, establishment, survival and early growth of tree species, weed competition and costs for plantation establishment and early maintenance. Most species had low seedling emergence and establishment, but high survival rates, implying that low seedling emergence is the main barrier to community assembly that must be overcome. The most successful species had larger seeds, belonged to non-pioneer categories and had slower growth rates. Final costs after two years were lower than has been reported in the literature for most restoration planting using seedlings both in Brazil and elsewhere; however, seedling density was low. Although direct seeding may be a feasible alternative to decrease planting costs, the poor species performances and low seedling density may reduce its applicability. Thus, we recommend direct seeding only in association with the planting of pioneer species seedlings.

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Section: Group 1 -Suitable Speciesmentioning

confidence: 99%

Section: Technical Feasibilitymentioning

confidence: 99%

Direct seeding reduces costs, but it is not promising for restoring tropical seasonal forests

Souza

Engel

2018

Ecological Engineering

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“…Morong. ); Kaiser et al (2014) with pitanga (Brazilian cherry) (Eugenia uniflora L.); Gimenez et al (2014) with graviola (Annona cherimola Mill. ); Garlet et al (2015) with gold medallion tree (Cassia leptophylla Vogel.…”

Section: Introductionmentioning

confidence: 99%

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

et al. 2017

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Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.

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“…em câmara fria (5±2 °C e 90% de umidade relativa do ar) e câmara seca (15±2 °C e 60% de umidade relativa do ar), Andrade et al (2000) verificaram que, após 30 dias de armazenamento, a porcentagem inicial de emergência (67%) reduziu 16 e 91%, respectivamente. Kaiser et al (2014) observaram que sementes de Eugenia uniflora L. armazenadas por 30 dias em geladeira (10 °C) apresentaram 94% de germinação. Benefícios potenciais do armazenamento em ambiente refrigerado também foi observado para sementes de E. calycina (VON BÜLOW et al, 1994), E. involucrata DC.…”

Section: Resultsunclassified

QUALIDADE DE SEMENTES DE Eugenia dysenterica DC. DURANTE O ARMAZENAMENTO

Silva

Vale

Silva

et al. 2019

Reveng

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Palavras-chave:Conservação emergência recalcitrante RESUMO A adoção de estratégias adequadas para o armazenamento de sementes é essencial para conservação da sua qualidade, ainda mais para aquelas classificadas como recalcitrantes.Objetivou-se, com este trabalho, avaliar a qualidade de sementes de Eugenia dysenterica DC. durante o armazenamento em ambiente natural e refrigerado. Os frutos foram coletados diretamente no chão e despolpados manualmente, e as sementes lavadas em água corrente, selecionadas quanto ao tamanho e armazenadas por 0; 7; 14; 21 e 28 dias em ambiente natural de laboratório (26,6 °C e 52,2% URA) e em refrigerador (8,4 °C e 49,2% URA). Para cada ambiente e período de armazenamento, foram realizadas as seguintes análises: emergência; índice de velocidade; tempo médio e primeira contagem de emergência de plântulas; comprimento da parte aérea e da raiz principal; massa fresca e seca da parte aérea e das raízes; massa seca total das plântulas; índice de qualidade de Dickson e teor de água. Independe do ambiente estudado, a porcentagem de emergência de plântulas se manteve acima de 84%, mesmo após 28 dias de armazenamento. O armazenamento das sementes de E. dysenterica DC. reduz a velocidade de emergência e o desempenho das plântulas, principalmente para aquelas armazenadas em ambiente natural. ABSTRACTThe adoption of adequate strategies for the storage of seeds is essential for their quality's conservation, even more for those classified as recalcitrant. The aim of this work was to evaluate the quality of Eugenia dysenterica DC.'s seeds during storage in a natural and refrigerated environment. The fruits were collected directly on the ground and manually pulped. The seeds were washed in running water, selected by size and stored for 0, 7, 14, 21 and 28 days in a natural laboratory's environment (26.6 °C and 52 °C, 2% URA) and in a refrigerator (8.4 °C and 49.2% URA). The following analyzes were performed for each environment and storage period: emergency, speed index, average time, first emergence's count of seedlings, length of the aerial part and the main root, fresh and dry mass of the aerial part and the roots, total dry mass of seedlings, quality index of Dickson and water content. The percentage of emergence's seedlings was above 84%, even after 28 days of storage, regardless the studied environment. The storage of E. dysenterica DC.'s seeds reduces the emergency speed and the seedlings' performance, mainly for those stored in a natural environment.

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Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

Cited by 10 publications

References 25 publications

Direct seeding reduces costs, but it is not promising for restoring tropical seasonal forests

Direct seeding reduces costs, but it is not promising for restoring tropical seasonal forests

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

QUALIDADE DE SEMENTES DE Eugenia dysenterica DC. DURANTE O ARMAZENAMENTO

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