Nitrogen assimilation in the bromeliad Ananas comosus var. ananassoides (Baker) Coppens & F.Leal grown in vitro with different sources of inorganic nitrogen

Abstract

Ananas comosus var. ananassoides (Baker) Coppens & F.Leal is a native ornamental bromeliad of the endangered biome Cerrado. Therefore, approaches aimed at the preservation of this species, such as in vitro cultivation and micropropagation are needed. Nitrogen (N) is absorbed by plants, mainly as NO3- and/or NH4+, and assimilated into amino acids. The aim of this work was to evaluate the N assimilation in this bromeliad. Plants were grown in vitro for seven months in modified MS medium with 15, 30, 60, and 90 mM of N as NO3-, NH4+ or NH4NO3, and then transferred to ex vitro conditions for acclimatization. Plants grown with NH4+ had high mortality. During acclimatization plants cultivated with 30, 60, and 90 mM of N as NH4NO3 showed higher biomass. With regard to N assimilation, GS and NR showed the highest activity in plants cultivated with NH4NO3, whereas plants cultivated with NH4+ had the highest GDH activity. Consequently, in vitro and ex vitro cultivation of this species with 60 mM N as NH4NO3 is recommended.

Initial in vitro plant establishment of seeds and nodal segments from bromeliad Acanthostachys strobilacea (Schult. & Schult.f.) Klotzsch differs in respiratory rates and shoot formation

“…Em conclusão, o processo de desenvolvimento das plantas de NS pode afetar a demanda energética e respiratória de forma diferente daquelas de sementes Palavras-chave: dióxido de carbono, gemas laterais, micropropagação, oxigênio, respiração possibility of maintaining cultures in controlled conditions (light, temperature, nutrients, etc. ), this technique has been used for studies of basic physiology in diverse species (Narayani & Srivastava 2017, Bakhshipour et al 2019, Chetty et al 2020, including bromeliads (Carvalho et al 2013, Freitas et al 2015, Andrade & Tamaki 2016, Santos et al 2017, Silva et al 2017, Andrade-Santos et al 2020, Silva et al 2020.…”