Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

Bich, Gustavo Ángel; Castrillo, María Lorena; Kramer, Fernando Luis; Villalba, Laura Lidia; Zapata, Pedro Darío

doi:10.1590/2179-8087-floram-2018-0086

Cited by 16 publications

(4 citation statements)

References 29 publications

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“…MT448732.1), meaning that the isolates had a high phylogenetic relationship and were in the same species. Ribosomal DNA sequences were used to determine the phylogenetic relationships of organisms to taxa species (Bich et al 2021). Henry et al (Henry et al 2000) stated that the similarity value of 99% shows that the isolates are the same species.…”

Section: Discussionmentioning

confidence: 99%

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

et al. 2022

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Fungi from South Sumatra (Indonesia) were identified morphologically and molecularly, and their pathogenicity to egg, larvae, and adult Aedes aegypti was evaluated. The fungal isolates used for bioassay were 11 isolates from this study and 4 isolates from the laboratory collection. Fifteen isolates of five fungal species (Metarhizium anisopliae, Penicillium citrinum, Talaromyces diversus, Beauveria bassiana, and Purpureocillium lilacinum) from South Sumatra, Indonesia, were pathogenic to the egg, larvae, and adult of Ae. aegypti. Egg mortality caused by M. anisopliae isolate MSwTp3 was the highest (38.31%). A novel finding of this study was that the eggs exposed to the fungus not only killed the eggs but could continue to kill the emerging larvae, pupae, and adults. The five fungal species induced larval mortality between 52.22−94.44% and adult mortality between 50.00−92.22%. Fungal strains belonging to M. anisopliae, P. citrinum, T. diversus, and B. bassiana from South Sumatra seem to possess remarkable ovicidal, larvicidal and adulticidal activity against Ae. aegypti. M. anisopliae, P. citrinum, T. diversus, and B. bassiana had the potential as entomopathogens to be developed into ovicides, larvicides, and adulticides for controlling Ae. aegypti.

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Section: Discussionmentioning

confidence: 99%

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

et al. 2022

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“…The sequence of ITS region of 18S rDNA of Metarhizium PB-75 was 1,218 base pairs in length, with a molecular weight at 367,667.00 for single-stranded and 739,779.00 Daltons for doublestranded, with slightly high AT content (53.28%). Ribosomal DNA sequences are commonly used to identify and determine the phylogenetic relationships of organisms to taxa species [62]. The organisms which have a similarity value of 100% can be stated as the same strain and a similarity value of 99% is stated as the same species, while the similarity value of 89-99% belongs to the same genus [63].…”

Section: Similarity Of Its Region Of 18s Rdna Of Metarhizium Pb-75 Co...mentioning

confidence: 99%

Occurrence of Entomopathogenic Fungi from Natural Ecosystems in Phetchabun, Thailand and Their Virulence Against Brown Planthopper

Bunsak

Homhaul

Pongprasert

et al. 2022

Curr. Appl. Sci. Technol.

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Brown planthopper (BPH), Nilaparvata lugens Stål, is a serious insect pest of rice. The invasion of BPH causes heavy losses in rice production, both quality and quantity. The objective of this research was to search for Metarhizium spp., a genus of entomopathogenic fungi in the Clavicipitaceae family, from forest soil in three districts of Phetchabun Province, Thailand: Khao Kho, Lom Kao and Nam Nao to control BPH. Metarhizium spp. fungi were isolated by soil dilution plate technique and determined their efficacy using conidia suspension at the concentration of 1X108 conidia/mL on BPH at the 2-3th instar nymph fed on seedlings of susceptible rice cultivars, Taichung Native 1 (TN1). The most effective Metarhizium sp. isolate was identified using the ITS region of 18S rDNA sequencing, Basic Local Alignment Search Tool (BLAST) and evolutionary history. The result showed that 126 isolates of Metarhizium spp. were found and coded as PB-01 to PB-126. All isolates infected BPH nymph with 26.7 to 100% mortality within 6 days after contacting conidia suspension. The isolate of PB-75 showed the highest efficacy (100% BPH mortality) with the lethal times of 50% mortality (LT50) within 2.9 days. The species identification showed that the DNA sequence of Metarhizium sp. isolate, PB-75, was 98.6% similar to M. anisopliae Genbank ID JQ889704.1. Evolutionary history based on phylogenetic analysis using neighbor-joining and maximum parsimony methods confirmed that PB-75 formed the same phylogenetic clade with M. anisopliae Genbank ID JQ889704.1 and M. anisopliae var. anisopliae.

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“…Particularmente, la cepa T. harzianum TrichoX utilizada como control experimental presentó una capacidad cuantitativa de inhibición del crecimiento del fitopatógeno cercana al 50% y un índice de antagonismo de Bell et al (1982) de clase 1. Esto brinda una prueba de la utilidad y las ventajas que se pueden obtener al utilizar los recursos disponibles regionalmente (Raza et al, 2019;Canassa et al, 2020;Laczeski et al, 2020), aprovechando la gran biodiversidad de la selva paranaense, la cual guarda un gran potencial para múltiples aplicaciones biotecnológicas con microorganismos (Gil, 2007;Bich et al, 2021).…”

Section: Capacidad Biocontroladora In Vitro De Aislamientos De Tricho...unclassified

CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)

Castrillo¹,

Bich²,

Sioli³

et al. 2021

Chil. j. agric. anim. sci.

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Los hongos fitopatógenos afectan a cultivos agrícolas, como la yerba mate (Ilex paraguariensis Saint Hil.). Una alternativa innovadora para el control de plagas es el empleo de hongos biocontroladores del género Trichoderma. Los objetivos del presente trabajo fueron aislar los agentes fúngicos causantes de lesiones foliares en yerba mate, y evaluar la capacidad antagónica de los aislamientos nativos de Trichoderma sp. frente a patógenos en este cultivo. A partir del corte, desinfección y siembra del tejido vegetal enfermo, se aisló e identificó morfológica y molecularmente un hongo fitopatógeno perteneciente a la especie Alternaria alternata. Se ensayaron pruebas de patogenicidad en plantas de yerba mate por triplicado. Se realizaron lesiones mecánicas superficiales en ellas y se inoculó el aislamiento fitopatógeno obtenido (conidios y micelio) para evaluar su capacidad fitopatógena. Se determinaron nuevas variantes de cultivo dual para evaluar la capacidad antagónica biocontroladora en condiciones in vitro. Se evaluó la capacidad biocontroladora in vitro de 30 aislamientos de Trichoderma frente al aislamiento fitopatógeno obtenido. Como control positivo se utilizó una cepa aislada de un producto fungicida comercial a base de T. harzianum. Se determinó que 18 de los 30 aislamientos de Trichoderma lograron inhibir en más del 50% el crecimiento del fitopatógeno A. alternata y presentaron diferencias estadísticamente significativas con respecto al

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Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

Cited by 16 publications

References 29 publications

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

Entomopathogenic Fungi from South Sumatra (Indonesia) Pathogenicity to Egg, Larvae, and Adult of Aedes aegypti

Occurrence of Entomopathogenic Fungi from Natural Ecosystems in Phetchabun, Thailand and Their Virulence Against Brown Planthopper

CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)

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