In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

Abstract

The extraction of native orchids from natural habitats is relevant for the reduction of populations in the Cerrado biome, making it necessary to establish practices aiming their production both for reintroduction and commercialization. The objective here is to evaluate light sources and sealing systems on the in vitro and ex vitro growth of Schomburgkia crispa. Two flask sealing systems were tested: conventional (CSS) and with gas exchange (SSGE), and eight light sources: FL1-100% white LED, FL2-100% blue LED, FL3-100% red LED, FL4-50% white + 25% red + 25% blue LED, FL5-50% red + 50% blue LED, FL6-25% red + 75% blue LED, FL7-75% red + 25% blue LED, and FL8- with fluorescent lamp, with five replications in each treatment. A completely randomized design was adopted with a 2x8 factorial scheme (vial sealing system x light sources). After 120 days of cultivation in vitro and 180 days ex vitro, the plants were evaluated as for number of leaves, roots and shoots, plant height, pseudobulb diameter, length of the largest root, largest leaf, and fresh mass. For the in vitro growth, the use of SSGE together with the light sources blue and red favors the cultivation of S. crispa. For the ex vitro growth, the cultivation in vitro in SSGE together with FL4 affects the acclimatization of plants.

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

“…Plant tissue culture serves as a platform for plant cloning under aseptic conditions and environmental control in the laboratory [15]. Traditionally, in vitro multiplication relies on three nutritional types of cultures: heterotrophic, where there are no photosynthetically active organs, and the carbon source is solely the carbohydrate supplied in the medium; photomixotrophic, where photosynthetically active organs use the carbohydrate in the medium, along with the consumption of CO 2 in the flask headspace; and photoautotrophic (or sugar-free medium), where photosynthetically active organs rely on CO 2 in the flask headspace as the carbon source [16,17]. The latter two cultivation systems, photoautotrophic and photomixotrophic, promote in vitro plant growth compared to the first (heterotrophic), which is more suitable for callus cultivation [16].…”

“…While photoautotrophic cultivation requires the use of gas-permeable films to enhance gas exchange between the flask headspace and the external environment, these films can also be employed in photomixotrophic (sugar-provided medium) cultivation to improve the ventilation rate of the culture flask [17][18][19]. Investigating the impact of natural ventilation on both photoautotrophy and photomixotrophy in the in vitro cultivation of Eryngium foetidum plants may offer valuable insights into the morphophysiological characteristics influencing the growth and development of plants in these systems.…”