Proline and antioxidant enzymes protect Tabebuia aurea (Bignoniaceae) from transitory water deficiency

Abstract

Water deficiency is a major abiotic stress that limits biomass production and drives plant species distributions. We evaluate the effects of water deficiency on ecophysiological and biochemical parameters of seedlings of Tabebuia aurea. Plants were subjected to daily watering (control) and to stress by soil water deficiency for 29 days. Leaf area, plant biomass, gas exchange, SPAD index, maximum quantum yield (Fv / Fm), quantum yield of PSII (ΦPSII), superoxide dismutase (SOD) and L-ascorbate peroxidase (APX) activity, lipid peroxidation, and proline content were recorded. Plants responded to water deficit by reducing leaf area and accumulating proline. Stomatal conductance was reduced to limit the water loss by transpiration. However, limiting CO2 uptake caused reduction in photosynthesis and biomass. The excess of energy unutilized by photosynthesis reduced SPAD index and ΦPSII. As a result, we observed an increase in SOD and APX activity, protecting chloroplast membranes from further damages caused by lipid peroxidation. Our results indicate that T. aurea have capacity to survive under water deficiency reducing stomatal aperture, but affecting the rate of CO2 assimilation. Nevertheless, plants showed mechanisms to preventing damages to the photosynthetic apparatus. Such plasticity is an important adaptation for plants growing in dry environmental.

Influence of desiccation and water deficit on the initial development of seedlings of Tabebuia aurea (Silva Manso) Beth. Hook. and S. Moore (Bignoniaceae)

“…Tolerance to desiccation is a remarkable skill of orthodox seeds. In the group of angiosperms this capacity decreases as the seed absorbs water and expresses germination [12]. Bowdichia virgilioides Kunth (Fabaceae) Enterolobium contortisiliquum (Vell.)…”

“…ex. S. Moore can attenuate stress via stomatic regulation with higher production of proline and antioxidant enzymes, with these conditions also resulting in a lower biomass accumulation [12].…”