Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

Abstract

In vitro grown plants may have abnormal structural and physiological features. However, the type of the sealing material used in tissue culture may minimize such abnormalities. This study evaluates the influence of the type of sealing of test tubes on the anatomical and ultrastructural features of leaves of Aechemea bromeliifolia (Rudge) Baker (Bromeliaceae), an ornamental bromeliad native to Brazil, grown in vitro. Three types of sealing were used: rigid polypropylene cap (PC), polyvinyl chloride film (PVC), and PC coupled with a microporous membrane (PM). Seedlings germinated in a greenhouse were also studied for comparison. Plants grown in test tubes sealed with PM were more similar to those from the greenhouse, as far as the pattern of stomatal opening, the presence of starch grains, and the organization of the internal membrane system of the chloroplasts is concerned. Plants cultivated in test tubes sealed with PC had higher stomatal density and the chloroplasts had large areas without thylakoids in the stroma. Plants grown in test tubes sealed with PVC had few or no starch grains. These results suggest that microporous membrane used coupled with PC sealing provided natural ventilation, thus contributing to a better plant development.

Photosynthetic apparatus performance and anatomical modulations of Alcantarea imperialis (Bromeliaceae) exposed to selenium during in vitro growth

“…In this work, the leaf and root anatomy of the plants were in accordance with previous reports of A. imperialis as well as other species of the Bromeliaceae family (Zorger et al 2019, Martins et al 2020a, Silva et al 2020b, Faria et al 2021. Even though Se modulated the root architecture of A. imperialis plants, this metalloid did not interfere with the roots' anatomical traits.…”

Introdução E Reavaliação De Aechmea Bromeliifolia (Rudge) Baker Para Fins De Enriquecimento Em Area Restaurada No Bioma Cerrado

“…Também conhecida como gravatá de tingir, possui cerca de 50 a 100 cm de altura, com inflorescência simples, espigada, fértil, elipsoide ou cilíndrica, com flores amarelos esverdeados (MOLIZANE et al, 2013). A anatomia foliar da espécie já foi descrita, apresentando tricomas glandulares estratificados, cutícula fina, folha hipoestomática e mesofilo contendo parênquima aquífero e colênquima (SILVA et al, 2020). Pertencente a Bromelioideae (Bromeliaceae), se distribui em praticamente todos os estados brasileiros.…”