Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Araújo, Maria da Conceição da Rocha; Chagas, Edvan Alves; Vendrame, Wagner A.; Ribeiro, Maria Isabel; Moura, Elias Ariel de; Taveira, D. L. L.; Chagas, Pollyana Cardoso; Grigio, Maria Luiza

doi:10.1590/1984-70332021v21n3a40

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“…Several factors influence somatic embryogenesis induction, such as genotype (Dias et al 2018) and the source of explants (Ara ujo et al 2021). In our study, somatic embryo induction was observed only in PEMs induced from stem nodal segments, which were more responsive to all plant growth regulators in greater concentrations (range, 4-6 mgÁL -1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Somatic embryogenesis is a widely used technique for large-scale in vitro propagation of many species (Von Arnold et al 2002). The process of somatic embryo induction and plant regeneration is influenced by several factors, such as species, explant source, culture medium, and type and concentration of auxins and cytokinins (Araujo et al 2016;Ara ujo et al 2021;Gulzar et al 2020;W ojcikowska and Gaj 2017).…”

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confidence: 99%

“…The only study of in vitro culture of M. dubia reported 99% callus and 93% proembryogenic mass (PEM) formation (Ara ujo et al 2021). The best results in the study were obtained using woody plant medium (WPM) (Lloyd and McCown 1980) culture medium supplemented with 4 mgÁL -1 2,4-dinitrophenoxyacetic acid (2,4-D) and 1 mgÁL -1 benzylaminopurine (BAP).…”

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Somatic Embryogenesis of Myrciaria dubia (Kunth.) McVaugh

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Myrciaria dubia (caçari or camu-camu) is a species with great relevance because of its high levels of bioactive compounds and antioxidant activity. The species is propagated mainly by seed, which leads to high genetic diversity. Producing plants that maintain their characteristics on a large scale at a low cost is a challenge for fruit species, especially in the Myrtaceae family. Therefore, this study aimed at assessing the effects of different plant growth regulators at various concentrations for the induction of somatic embryogenesis from caçari nodal segments and leaf disk explants. Two independent experiments were performed using nodal segments and leaf disks from plants grown in the greenhouse. In the first experiment, the combined effect of auxin and cytokinin at different concentrations was evaluated: 2,4-dichlorophenoxyacetic acid at 0, 1, 2, and 4 mg⋅L–1; and benzylaminopurine at 0, 0.25, 0.5, and 1 mg⋅L–1). In the second experiment, the application of different plant growth regulators (benzylaminopurine, kinetin, thidiazuron, and isopentenyl adenine) and their concentrations (0, 2, 4, and 6 mg⋅L–1) were evaluated. In both experiments, the basic culture medium was woody plant medium. Callus formation via nodal segments and leaf disks occurred in the first 30 d from cultivation and proved to be responsive to induction by 2,4-dichlorophenoxyacetic acid and benzylaminopurine. Auxin proved to be essential for somatic embryogenesis induction in nodal segments using 1 and 4 mg⋅L–1 of 2,4-dichlorophenoxyacetic acid alone. The results of this experiment will help advancing protocols for regeneration of somatic embryos and elucidating the physiological, molecular, and genetic mechanisms involved in the process of somatic embryogenesis for M. dubia. The development of an efficient protocol for in vitro clonal propagation of this species also lays the groundwork for further optimization of the system for genetic transformation.

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confidence: 99%

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confidence: 99%