Breeding for PVY resistance in tobacco LJ911 using CRISPR/Cas9 technology

Ruyi, Ren; Zheng, Qiang; Fu-tai, NI; Qiu, Jing; Wan, Xin; Wei, Jicheng

doi:10.1590/1984-70332021v21n1a6

Cited by 6 publications

(2 citation statements)

References 20 publications

(12 reference statements)

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“…Tobacco, an important cash crop, is severely affected by potato virus Y, which is due to the susceptibility of gene Ntab0942120. Through the use of CRISPR/Cas9 technique, this gene was knockout and transgene-free homozygous edited plants were generated which showed resistance to PVY [171]. In brassica.…”

Section: Development Of Disease Resistant Varieties Using Crispr/cas9mentioning

confidence: 99%

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Rasheed

Gill

Hassan

et al. 2021

CIMB

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Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating the genomes of living organisms. Many tools have been developed in recent years to enable the editing of complex genomes. Therefore, a reliable and rapid approach for increasing yield and tolerance to various environmental stresses is necessary to sustain agricultural crop production for global food security. This critical review elaborates the GE tools used for crop improvement. These tools include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), and transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this review addresses the latest advancements in the role of CRISPR/Cas9 for genome manipulation for major crop improvement, including yield and quality development of biotic stress- and abiotic stress-tolerant crops. Implementation of this technique will lead to the production of non-transgene crops with preferred characteristics that can result in enhanced yield capacity under various environmental stresses. The CRISPR/Cas9 technique can be combined with current and potential breeding methods (e.g., speed breeding and omics-assisted breeding) to enhance agricultural productivity to ensure food security. We have also discussed the challenges and limitations of CRISPR/Cas9. This information will be useful to plant breeders and researchers in the thorough investigation of the use of CRISPR/Cas9 to boost crops by targeting the gene of interest.

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Section: Development Of Disease Resistant Varieties Using Crispr/cas9mentioning

confidence: 99%

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Rasheed

Gill

Hassan

et al. 2021

CIMB

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“… Mutation of nCBP [ 45 ] CBSV and UCBSV VPg Manihot esculenta ? Mutation of 4E (eIF4E) and nCBP [ 46 ] PVY VPg Nicotina tabacum va eIF4E (iso) [ 47 ] YoMV, ToMV, TMV, TMGMV, PMMoV Nicotiana spp., Solanum lycopersicum, C. annuum, O. sativa TOM1; TOM3 EMS mutagenesis [ 37 , 48 ] PepLCIV, PepLCAV C. annuum pepy1 Silencing of CaPelota [ 49 ] ToMV, YoMV A. thaliana, N. tabacum ARL8 Simultaneous mutation of ARL8a and ARL8b by T-DNA insertion [ 37 , 50 ] TuMV, PPV A. thaliana, N. tabacum, Zea mays, O. sativa, Mesembryanthemum crystallinum DBP1 T-DNA mutant [ 51 ] WMV, PPV, BaMV S. lycopersicum, S. tuerosum, Populus trichocarpa, Sorghum bicolor, O. sativa, Tritichum aestivum, Z. mays cPGK Natural resistance gene, rwm1 in Arabibopsis thaliana CVI-0 ecotype [ 37 , 52 , 53 ...…”

Section: Main Textmentioning

confidence: 99%

Resistance induction based on the understanding of molecular interactions between plant viruses and host plants

Akhter

Nakahara

Masuta

2021

Virol J

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Background Viral diseases cause significant damage to crop yield and quality. While fungi- and bacteria-induced diseases can be controlled by pesticides, no effective approaches are available to control viruses with chemicals as they use the cellular functions of their host for their infection cycle. The conventional method of viral disease control is to use the inherent resistance of plants through breeding. However, the genetic sources of viral resistance are often limited. Recently, genome editing technology enabled the publication of multiple attempts to artificially induce new resistance types by manipulating host factors necessary for viral infection. Main body In this review, we first outline the two major (R gene-mediated and RNA silencing) viral resistance mechanisms in plants. We also explain the phenomenon of mutations of host factors to function as recessive resistance genes, taking the eIF4E genes as examples. We then focus on a new type of virus resistance that has been repeatedly reported recently due to the widespread use of genome editing technology in plants, facilitating the specific knockdown of host factors. Here, we show that (1) an in-frame mutation of host factors necessary to confer viral resistance, sometimes resulting in resistance to different viruses and that (2) certain host factors exhibit antiviral resistance and viral-supporting (proviral) properties. Conclusion A detailed understanding of the host factor functions would enable the development of strategies for the induction of a new type of viral resistance, taking into account the provision of a broad resistance spectrum and the suppression of the appearance of resistance-breaking strains.

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