QTL mapping of spike fertility index in bread wheat

Abstract

Spike fertility index (SF) is a trait easily measured at maturity and strongly associated with the number of grains per unit area. In order to identify genomic regions involved in SF control, a biparental (Baguette 10 × Klein Chajá) population of 80 recombinant inbred lines (RIL) was used. Seven field trails were conducted to determine the SF BLUP value per RIL. RILs were genotyped using a commercial chip (Axiom® 35K SNP Wheat Breeder's Array, Affimetrix). A linkage map was constructed with 857 SNP markers, and SF QTL mapping was performed. The narrow-sense heritability of SF was 0.89. Three genomic regions (QTL) associated with SF were found on chromosomes 2D, 4A, and 7A. The proportion of genetic variation explained by these three QTL was 32%, with no significant epistatic interaction between QTL.

QTL mapping for Type II resistance to Fusarium head blight and spike architecture traits in bread wheat

“…These results give further validity to the QTL detection process carried out in the present study. Results of molecular marker analysis and map construction were described in Alonso et al (2021). As discussed by these authors, very stringent conditions were applied to the map construction and QTL mapping procedures, in order to overcome the limitations of the use of a population of reduced size.…”

“…Of 126 RIL, 80 lines with wide phenotypic variability for all traits were randomly chosen for marker analysis. Molecular marker analysis and map construction were described in Alonso et al (2021). Briefly, molecular genotyping was carried out with a commercial "chip", Axiom® 35K SNP Wheat Breeder's Array (Affimetrix) of 35.042 SNP (single nucleotide polymorphism) markers.…”