Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)

Cid-Contreras, Roberto Carlos; Mascorro-Gallardo, José O.; Valadez-Moctezuma, Ernestina

doi:10.1590/1984-70332019v19n3a40

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“…In addition, they do not require previous knowledge of target sequences (Ng and Tan, 2015;Grover and Sharma, 2016). Consequently, ISSR is an eff ective tool for a variety of purposes, including identifi cation of germplasm and evaluation of genetic diversity (Coutinho et al, 2014;Kumar and Agrawal, 2017;Cid-Contreras et al, 2019;Al Salameen et al, 2020;Xiang et al, 2020).…”

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confidence: 99%

GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

et al. 2021

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The evaluation of accessions in a germplasm bank is essential for determining the potential parents in conservation programs, especially for native trees. This study aimed to determine the genetic diversity among 68 Amburana cearensis genotypes from different locations in the state of Pernambuco, Brazil. Their genetic patterns were evaluated by Inter Simple Sequence Repeat (ISSR) molecular markers and genetic divergence was evaluated through multivariate analyses using different clustering methods. The optimization method used (Tocher) was in agreement with all the hierarchical models used, in which clustering of the genotypes occurred similarly, specifically for the accession BB116, which is an important genetic material to be preserved and studied. Among the various hierarchical methods applied, the Average Linkage method exhibited higher discrimination power, allowing identification of a larger number of divergent groups, thus implying wide genetic diversity among A. cearensis accessions.

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Section: Introductionmentioning

confidence: 99%

GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

et al. 2021

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Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)

Abstract: Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19:

Cited by 1 publication

References 24 publications

GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

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