Development and validation of SNP assays for the selection of resistance to Meloidogyne incognita in soybean

Abstract

This work aimed to develop and validate individual SNP molecular markers previously identified in a genetic association study of resistance to Meloidogyne incognita in soybean using a microarray panel. The markers identified in the array were converted in single TaqMan® markers. The single markers were used to create an SNP genotyping protocol and establish a marker-assisted selection (MAS) routine associated with resistance to M. incognita in soybean. Out of the eight TaqMan® assays tested, three were validated for use in MAS. The MAS protocol developed in this study uses sequential selection. Initially, molecular markers are used to identify susceptible plants; subsequently, the phenotypic evaluation of plants expressing resistance genotype for the markers is carried out, resulting in the accurate identification of resistant plants. The accuracy of this approach for MAS sequential for M. incognita varied from 94 to 96%.

Genetic Mapping Reveals Complex Architecture and Candidate Genes Involved in Common Bean Response to Meloidogyne Incognita Infection

“…In addition, several candidate genes were indicated to putatively control the resistance, including 12 RGAs and five DE genes in common bean roots after inoculation. Thus, validation by fine mapping approaches might provide valuable information for marker-assisted selection and aid breeders to face the challenges imposed by the phenotyping bottleneck (Caromel and Gebhardt 2011), as is already the case for peanut (Chu et al 2011), cotton (Jenkins et al 2012) and soybean crops (Dubiela et al 2019). Finally, functional analysis of the candidate genes will help elucidate the molecular basis of the P. vulgaris-M. incognita interaction and could eventually be used for genetically modifying other related crops.…”

Genetic mapping reveals complex architecture and candidate genes involved in common bean response to Meloidogyne incognita infection

“…In addition, several candidate genes were indicated to putatively control the resistance, including 14 RGAs and five DE genes in common bean roots after inoculation. Thus, validation by fine mapping approaches might provide valuable information for marker‐assisted selection and aid breeders to face the challenges imposed by the phenotyping bottleneck (Caromel & Gebhardt, 2011), as is already the case for peanut (Chu et al., 2011), cotton (Jenkins et al., 2012), and soybean crops (Dubiela et al., 2019). Finally, functional analysis of the candidate genes will help elucidate the molecular basis of the P. vulgaris – M. incognita interaction and could eventually be used for genetically modifying common bean or other related crops.…”

Single Nucleotide Polymorphisms: A Modern Tool to Screen Plants for Desirable Traits

“…SNP markers have been frequently used in marker assisted selection due to its abundance in the genes of all species. In breeding for resistance to root-knot nematode (Melodogyne incognita) that infects soybean by Dubiela et al [55], SNP markers were identified for M. incognita in soybean using a microarray panel. The markers were used to identify susceptible soybean which was confirmed by phenotypical evaluation of plants on the field.…”