A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

Li, Jun Zhou; Cao, Yong Run; Li, Meng Qi; Zhao, Weiying; Sun, Hong Zheng; Zhao, Quan

doi:10.1590/1984-70332017v17n3a38

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…Of these markers, exDEP1 didn't show ampli cation with any genotypes. DEP1INDEL1 and DEP1-1 produced amplicons of 1030bp (as in Kim et al, 2016) and 1860bp which indicates non-erect panicle type (as in Li et al, 2017), respectively in only rice genotypes (Supplementary Fig. 4) and no ampli cation was seen in peanut genotypes.…”

Section: Yield Contributing Genesmentioning

confidence: 99%

Transferability of Rice Yield and Seed Quality Gene Tagged Markers to Peanut (Arachis hypogaeaL.): A Way Ahead in Groundnut Improvement

Sukrutha

Srividhya

Vemireddy

et al. 2023

Preprint

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Background The progress in tagging/mapping of genes in crops like Arabidopsis, rice, maize etc. is far ahead when compared to the major legume crops viz. groundnut, chickpea, redgram, greengram, soyabean etc., even in the post release period of legume genome sequence databases. This can be attributed mainly to low level of available molecular genetic diversity in these crops. Methods The study of transferability of Rice yield gene tagged markers to Peanut was carried out by employing17 diverse groundnut genotypes. ANOVA revealed significant differences for six yield and seven seed quality traits studied which indicates availability of ample amount of variability among the genotypes. Molecular analysis was conducted to analyse the transferability of known rice yield, grain size and micronutrient content (Fe and Zn) controlling gene tagged markers (GTMs) to peanut by using 45 GTMsthat targets 24 known functional genes. Results Of 45 markers, 31 (76%) were transferable to peanut, denotes very high conservation at functional regions. The extent of amplification of rice GTMs at individual groundnut genotype level was observed from 79.17% for the cultivated varieties (2n = 4x) Nithya Haritha, Greeshma, Prasuna, Kalahasti, Narayani and with a wild genotype, Arachis villosa(2n = 2x) to 91.67% for Dheeraj variety. The analysis on transferable efficiency of individual rice GTM revealed that 17 markers belong to 14 rice genes showed amplification among all the 17groundnut genotypes (100%) under study. Conclusions Hence, focusing research on the available knowledge of functionally characterized genes of molecular model crops and vast list of annotated orthologous genes present in ‘Omics’ databases, widens the scope to tag the genes at molecular level and thereby to improve the cropslike groundnutthat has meager progress in gene tagging;by pyramiding of desirable genes with high veracity.

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Section: Yield Contributing Genesmentioning

confidence: 99%

Transferability of Rice Yield and Seed Quality Gene Tagged Markers to Peanut (Arachis hypogaeaL.): A Way Ahead in Groundnut Improvement

Sukrutha

Srividhya

Vemireddy

et al. 2023

Preprint

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Possibility for Exploitation and Identification of Rice Yield and Seed Quality Orthologs in Peanut (Arachis hypogaea L.)

Sukrutha

Akkareddy

Vemireddy

et al. 2023

Preprint

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Background The progress in tagging/mapping of genes in crops like Arabidopsis, rice, maize etc. is far ahead when compared to the major legume crops viz. groundnut, chickpea, redgram, greengram, soyabean etc., even in the post release period of legume genome sequence databases. This can be attributed mainly to low level of available molecular genetic diversity in these crops. Methods The study of transferability of Rice yield gene tagged markers to Peanut was carried out by employing17 diverse groundnut genotypes. ANOVA revealed significant differences for six yield and seven seed quality traits studied which indicates availability of ample amount of variability among the genotypes. Molecular analysis was conducted to analyse the transferability of known rice yield, grain size and micronutrient content (Fe and Zn) controlling gene tagged markers (GTMs) to peanut by using 45 GTMsthat targets 24 known functional genes. Results Of 45 markers, 31 (76%) were transferable to peanut, denotes very high conservation at functional regions. The extent of amplification of rice GTMs at individual groundnut genotype level was observed from 79.17% for the cultivated varieties (2n = 4x) Nithya Haritha, Greeshma, Prasuna, Kalahasti, Narayani and with a wild genotype, Arachisvillosa(2n = 2x) to 91.67% for Dheeraj variety. The analysis on transferable efficiency of individual rice GTM revealed that 17 markers belong to 14 rice genes showed amplification among all the 17groundnut genotypes (100%) under study. Conclusions Hence, focusing research on the available knowledge of functionally characterized genes of molecular model crops and vast list of annotated orthologous genes present in ‘Omics’ databases, widens the scope to tag the genes at molecular level and thereby to improve the cropslike groundnutthat has meager progress in gene tagging;by pyramiding of desirable genes with high veracity.

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The Development of Multi-Resistant Rice Restorer Lines and Hybrid Varieties by Pyramiding Resistance Genes against Blast and Brown Planthopper

Deng,

Qin,

Liu

et al. 2024

Agronomy

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Rice blast, caused by the fungus Magnaporthe oryzae, and brown planthopper (BPH) infestation are two of the most destructive problems of rice production in China. The development of multi-resistant varieties is widely recognized as the most efficient and environmentally friendly approach to controlling crop diseases and pests. Functional molecular markers (FMMs) have been developed from functional variants in the genic region associated with trait variation, greatly enhancing the efficiency of identifying and pyramiding valuable genes in crop breeding. In this study, two FMMs and a multiplex PCR amplification system were developed for two major broad-spectrum BPH resistance genes, Bph6 and Bph9. With the assistance of FMMs in the multi-resistant rice restorer-line development pipeline, two lines (Huahui7713 and Huahui3006) with blast and BPH resistance were developed by pyramiding three resistance genes Pigm, Bph6 and Bph9. Two new hybrid rice varieties, Weiliangyou7713 and Xuanliangyou3006, derived from Huahui7713 and Huahui3006, have been developed and commercialized in China. Weiliangyou7713 and Xuanliangyou3006 exhibit enhanced resistance to both blast and BPH, while maintaining optimal yield and grain quality. The adoption of Weiliangyou7713 continues to expand, now being cultivated on a large scale, which is promising for its future role in reducing the dependence on chemical fungicides and pesticides in rice production. This suggests that the implementation of Huahui7713 and Huahui3006 in targeted breeding programs could be highly beneficial for developing rice varieties with strong resistance to blast and BPH.

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A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

Cited by 3 publications

References 20 publications

Transferability of Rice Yield and Seed Quality Gene Tagged Markers to Peanut (Arachis hypogaeaL.): A Way Ahead in Groundnut Improvement

Transferability of Rice Yield and Seed Quality Gene Tagged Markers to Peanut (Arachis hypogaeaL.): A Way Ahead in Groundnut Improvement

Possibility for Exploitation and Identification of Rice Yield and Seed Quality Orthologs in Peanut (Arachis hypogaea L.)

The Development of Multi-Resistant Rice Restorer Lines and Hybrid Varieties by Pyramiding Resistance Genes against Blast and Brown Planthopper

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