Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants

Picolotto, Dayana Rotili Nunes; Neto, Vespasiano Borges de Paiva; Barros, Fábio de; Padilha, Daly Roxana Castro; Cruz, Ana Cláudia Ferreira da; Otoni, Wagner Campos

doi:10.1590/1984-70332017v17n3a30

Cited by 15 publications

(10 citation statements)

References 22 publications

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“…Despite a significant amount of studies with IPR-PLB in different orchid species and hybrids, such as Coelogyne cristata and C. flaccida [33,34], Cyrtopodium paludicolum [35], Grammatophyllum speciosum [36] among others, this review has as its main objective to compile the recent studies and advances found in the induction, proliferation and regeneration of PLBs from the two most important genera in the world flower market, especially Phalaenopsis and Oncidium hybrid groups.…”

Section: Introductionmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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Section: Introductionmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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“…Shoot buds may develop from any plant tissue for a large number of species and several researches have been studying shoot buds regeneration from root segments of many species under in vitro conditions. A few species can produce shoot buds from root explants under in vitro conditions without plant growth regulators in the induction medium, as obtained by Delgado-Paredes et al (2016) in Ipomoea batatas and Picolotto et al (2017) in Cyrtopodium paludicolum.…”

Section: Introductionmentioning

confidence: 94%

Triiodobenzoic acid affects the regenerative competence in root explants of Adenocalymma nodosum (Bignoniaceae)

Neto

Oliveira

Sanches

et al. 2020

PCCM

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Adenocalymma nodosum, a native plant species from Brazilian Savanna, has a very interesting propagation mechanism by root segments, mainly, in areas with weed mechanical control or soil prepared by farming equipment such as plough that results in the cited root segments, enabling high regenerative capacity to generate adventitious shoots. This regenerative competence of root segments, without exogenous plant growth regulators, was previously showed to occur on in vitro conditions. Aiming to study the effect of the auxin transport inhibition on the regenerative capacity of the root segments of this species, root explants were placed in different orientations (horizontal, upright and inverted upright) on Murashige and Skoog medium supplemented or not with triiodobenzoic acid (TIBA). It was possible to verify that root explants have a polar response with shoot buds being directly differentiated from pericycle cells only at root proximal ends. Use of TIBA, an auxin transport inhibitor, inhibited organogenesis and induced callogenesis, showing that auxin transport is essential to the favorable hormonal balance to bud differentiation at the explant proximal end.

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“…Although, much is known about germination and tissue culture, few attempts have been made with the genus Cyrtopodium (ARAÚJO, et al, 1999;DUTRA, et al, 2009;PEREIRA et al, 2015;PICOLOTTO et al, 2017;SOUSA et al, 2019) because it has been underutilised as an ornamental plant.…”

Section: Introductionmentioning

confidence: 99%

In vitro development and acclimatization of Cyrtopodium aliciae L. Linden & Rolfe, an endemic species of the Chapada Diamantina

et al. 2023

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Orchids are valued as ornamental plants, bioindicators, and medicinal plants, which implies that some species may be over-collected. Some inhabit very fragile environments and are under threat by the misuse of habitats and anthropogenic impacts. The search for beautiful plants and flowers has increased the number of facilities for micropropagation either by seeding or by cloning plants using in vitro techniques. However, not all species have appropriate media for growth and development that would help in conservation efforts. Cyrtopodium aliciae is an endemic species of rupestrian grassland in Brazil t. It has appeal as an ornamental plant or for use in hybridisation programs dueo its small size and white brownish-purple dotted flowers. This study compared three different media, namely ½ concentration Murashige and Skoog (MS), Vacin and Wendt, and Knudson C, during plant growth and their effect on the acclimatization of Cyrtopodium aliciae. The number and length of shoots and roots, increase in mass, and survival in vitro and ex vitro were analyzed. The experiment was conducted as completely random with a factorial arrangement of treatments (3 × 3) with 10 repetitions per treatment containing 10 plants for the in vitro experiment and 3 repetitions of 10 plants for the ex vitro experiment. Cyrtopodium aliciae performed better in the ½ concentration MS medium with a higher increase in mass, plant development, and survival under both in vitro and ex vitro conditions.

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Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants

Cited by 15 publications

References 22 publications

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Triiodobenzoic acid affects the regenerative competence in root explants of Adenocalymma nodosum (Bignoniaceae)

In vitro development and acclimatization of Cyrtopodium aliciae L. Linden & Rolfe, an endemic species of the Chapada Diamantina

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