Efficient and new method for Tectona grandis in vitro regeneration

Tambarussi, Evandro Vagner; Rogalski, Marcelo; Galeano, Esteban; Brondani, Gilvano Ebling; Martín, Valentina; Silva, Lucas Américo da; Carrer, Helaine

doi:10.1590/1984-70332017v17n2a19

Cited by 12 publications

(8 citation statements)

References 43 publications

(45 reference statements)

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“…Teak ( Tectona grandis L.f.) (Lamiaceae family) is an important wood source in the tropics, with a significant market around the world. This species has wide and well-performed studies involving irrigation impacts on teak plantation phenology [13], leaf gas exchange in the field [14], molecular diversity [15–18], tissue culture advances [19–21], advanced molecular markers [22], gene structure and function [23–26], transcriptome studies [27], and the first genome examinations [28,29]. In terms of the biochemistry and physiology related to drought stress, some studies have showed the impacts on its development [30–34].…”

Section: Introductionmentioning

confidence: 99%

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

et al. 2019

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Drought stress is an increasingly common and worrying phenomenon because it causes a loss of production in both agriculture and forestry. Teak is a tropical tree which needs alternating rainy and dry seasons to produce high-quality wood. However, a robust understanding about the physiological characteristics and genes related to drought stress in this species is lacking. Consequently, after applying moderate and severe drought stress to teak seedlings, an infrared gas analyzer (IRGA) was used to measure different parameters in the leaves. Additionally, using the root transcriptome allowed finding and analyzing the expression of several drought-related genes. As a result, in both water deficit treatments a reduction in photosynthesis, transpiration, stomatal conductance and leaf relative water content was found. As well, an increase in free proline levels and intrinsic water use efficiency was found when compared to the control treatment. Furthermore, 977 transcripts from the root contigs showed functional annotation related to drought stress, and of these, TgTPS1, TgDREB1, TgAREB1 and TgPIP1 were selected. The expression analysis of those genes along with TgHSP1, TgHSP2, TgHSP3 and TgBI (other stress-related genes) showed that with moderate treatment, TgTPS1, TgDREB1, TgAREB1, TgPIP1, TgHSP3 and TgBI genes had higher expression than the control treatment, but with severe treatment only TgTPS1 and TgDREB1 showed higher expression than the control treatment. At the end, a schematic model for the physiological and molecular strategies under drought stress in teak from this study is provided. In conclusion, these physiological and biochemical adjustments in leaves and genetic changes in roots under severe and prolonged water shortage situations can be a limiting factor for teak plantlets’ growth. Further studies of those genes under different biotic and abiotic stress treatments are needed.

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Section: Introductionmentioning

confidence: 99%

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

et al. 2019

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“…In this species, in large-scale in vitro production the use of axillary buds allows a high sustainability of the culture and genotypic fidelity compared to de novo procedures; to date, millions of plants have been propagated this way [1]. Few studies have reported on the regeneration of teak plants via organogenesis [11] [12] or somatic embryogenesis [13].…”

Section: Introductionmentioning

confidence: 99%

“…In teak, we seek a balance between the number and length of shoots to continue multiplication cycles in order to produce good quality shoots for the ex vitro rooting and acclimation in the greenhouse.Rooting is one of the most difficult phases during the micropropagation of woody species; in trees, it is usually done ex vitro due to the low response of in vitro rooting[14]. Although numerous researchers have successfully practiced the in vitro rooting of teak shoots in semi-solid medium[3] [12] [51][55]; others have opted for rooting ex vitro[8] [9][14]…”

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Simple Protocol for the Micropropagation of Teak (Tectona grandis Linn.) in Semi-Solid and Liquid Media in RITA&reg; Bioreactors and ex Vitro Rooting

Aguilar¹,

Garita²,

Kim³

et al. 2019

AJPS

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In Latin America the forestry of exotic species such as teak has been increasing in recent decades, due to their advantages in wood quality, rapid growth; and the relative ease of producing clones and their multiplication with respect to native species. Therefore, there is great interest in developing larger-scale propagation strategies that reduce costs and intensive manual labor. Culture in liquid media with temporary immersion and the semi-automation of the system has raised expectations for large-scale micropropagation. We report a protocol for teak, which reuses the primary explants in several culture cycles in semi-solid medium to produce nodal explants for the multiplication phase in temporary immersion bioreactors (RITA ®). The control of factors such as cytokinin concentration, explants density, immersion frequencies and culture duration was analyzed. The number of shoots increased with 0.5 mg•l −1 of BA (6-Benzyladenine), alone or in combination with 0.5 mg•l −1 of Kinetin, with 2 daily immersions of 1 minute each; however, these shoots showed a high degree of hyperhydricity. When 0.05 mg•l −1 of BA was used with 1 immersion of 1 minute every 2 days, the hyperhydricity decreased. Although the number of shoots was lower, they showed good length to be used during multiplication and rooting ex vitro. Our results suggest that teak micropropagation can be simplified in two phases in vitro, the establishment and multiplication; followed by rooting ex vitro and acclimatization. This would imply a reduction in production costs, since most of the multiplication would take place in RITA ® containers.

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“…Notably, hypocotyls from Vernicia fordii (Mu et al 2016), Cyphomandra betacea (Kahia et al 2015), Fagopyrum esculentum (Hou et al 2015), Tectona grandis (Tambarussi et al 2017), nectarines (Yue et al 2007) and Eucalyptus dunnii (Lu et al 2016) are used routinely to produce regenerated plants. Besides, hypocotyls are excellent for genetic transformation as a result of their high rates of differentiation, convenience of selection, lack of seasonal restrictions and a high rate of infection with Agrobacterium (Huang 2006;Liao et al 2015;Liu et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient regeneration system for the precious and fast-growing timber tree Toona ciliata

Shang

Zhou

et al. 2018

Plant Biotechnology

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Toona ciliata (Chinese mahogany) is an important timber species and secondary protected plant due to excessive exploitation in China. Here we developed a robust and efficient regeneration system for adventitious shoot induction using hypocotyl explants of T. ciliata. To facilitate plant growth, different regulators were added to MurashigeSkoog (MS) medium (0.5 mg/l 6-BA, 1.0 mg/l KT and 0.1 mg/l IBA). A regeneration frequency of 58.67% with four shoots per explant was achieved by horizontal setting of hypocotyls on MS medium and following a 20-day seeding period. MS medium supplemented with 0.3 mg/l 6-BA and 0.2 mg/l NAA was optimal for shoot multiplication and elongation, with a multiplication coefficient of 3.06. A rooting frequency of 93.33% was achieved using the half-strength MS containing 0.1 mg/l NAA. After acclimatization, plantlets were transplanted to sterilized nutrient soil containing a 2 : 1 ratio of vermiculate with 90% survival frequency. Thus, the regeneration system developed in this study would be useful for genetic transformation and other biotechnology endeavours in T. ciliata.

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Efficient and new method for Tectona grandis in vitro regeneration

Cited by 12 publications

References 43 publications

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>&reg; </sup> Bioreactors and <i>ex Vitro</i> Rooting

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

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Efficient and new method for Tectona grandis in vitro regeneration

Cited by 12 publications

References 43 publications

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

Physiological and molecular responses to drought stress in teak (Tectona grandis L.f.)

Simple Protocol for the Micropropagation of Teak (&lt;i&gt;Tectona grandis&lt;/i&gt; Linn.) in Semi-Solid and Liquid Media in RITA&lt;sup&gt;&amp;reg; &lt;/sup&gt; Bioreactors and &lt;i&gt;ex Vitro&lt;/i&gt; Rooting

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

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Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>® </sup> Bioreactors and <i>ex Vitro</i> Rooting