UEL 175: a novel lipoxygenase-free soybean cultivar with kunitz trypsin inhibitor absence

Carpentieri-Pípolo, Valéria

doi:10.1590/1984-70332015v15n3c33

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…At ICAR–Indian Institute of Soybean Research, India, a soybean variety NRC 142, free from KTI and lipoxygenase 2, has been developed through marker-assisted breeding ( https://iisrindore.icar.gov.in/pdfdoc/NRC142News.pdf ). In Brazil, a KTI-free variety UEL 175 has also been developed through marker-assisted breeding ( 201 ).…”

Section: Genomic Approaches For Enhancing Nutritional Quality In Majomentioning

confidence: 99%

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

et al. 2020

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Nutritional stress is making over two billion world population malnourished. Either our commercially cultivated varieties of cereals, pulses, and oilseed crops are deficient in essential nutrients or the soils in which these crops grow are becoming devoid of minerals. Unfortunately, our major food crops are poor sources of micronutrients required for normal human growth. To overcome the problem of nutritional deficiency, greater emphasis should be laid on the identification of genes/quantitative trait loci (QTLs) pertaining to essential nutrients and their successful deployment in elite breeding lines through marker-assisted breeding. The manuscript deals with information on identified QTLs for protein content, vitamins, macronutrients, micro-nutrients, minerals, oil content, and essential amino acids in major food crops. These QTLs can be utilized in the development of nutrient-rich crop varieties. Genome editing technologies that can rapidly modify genomes in a precise way and will directly enrich the nutritional status of elite varieties could hold a bright future to address the challenge of malnutrition.

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Section: Genomic Approaches For Enhancing Nutritional Quality In Majomentioning

confidence: 99%

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

et al. 2020

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“…Both KTI and lipoxygenase‐2 genes are proteinaceous molecules, and their genetic elimination may cause changes in the level of protein content and storage protein fractions as suggested in earlier studies (Rani et al, 2020). Carpentieri‐Pipolo (2015) developed a specialty soybean UE 175 a lipoxygenase‐free soybean in KTI‐free background, but the authors did not investigate the removal of either of these genes on sprouting or the above‐mentioned relevant biochemical parameters. In the present study, Lox‐2 and KTI free soybean lines, developed by introgressing null alleles of Lox‐2 and KTI genes in the variety ‘JS97‐52’ through marker‐assisted backcross breeding, were investigated with the objective to study the effect of removal of each of these two undesirable genes from soybean seeds on the sprouting attributes, namely, number of normal seedlings emerged on first day, sprouting index (S.I.…”

Section: Introductionmentioning

confidence: 99%

Genetic elimination of lipoxygenase‐2 improves sprouting and tocopherols, and removal of Kunitz trypsin inhibitor enhances in vitro protein digestibility in soybean (Glycine max)

et al. 2022

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Genetic removal of lipoxygenase‐2 (Lox‐2) and Kunitz trypsin inhibitor (KTI) from soybean seeds is desired as the former generates off‐flavour associated with soy products while the latter affects protein digestibility. In the present study, Lox‐2 null and KTI null introgressed lines developed by introgressing null alleles of Lox‐2 and KTI in recurrent genotype JS97‐52 were assessed for sprouting attributes, storage protein fractions, in vitro protein digestibility (IVPD), tocopherol composition, thiobarbituric acid (TBA) number and Bowman–Birk inhibitor (BBI) in comparison with the recurrent genotype. Lox‐2 null introgressed line (NLx2IL) exhibited significantly (P < .05) higher first count, sprouting index, sprout length, tocopherol isomers and reduced TBA number. Null KTI introgressed line (NKTIIL) showed significantly (P < .05) enhanced IVPD and BBI concentration. The study showed that genetic elimination of lipoxygenase‐2 and KTI improved the sprouting and nutritional value of soybean, respectively.

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“…SSRs consist of small repeat units (1-6 bp) distributed in tandem throughout the genomes and are easily detected by polymerase chain reaction (PCR); they are relatively abundant, have extensive genome coverage, and require a low amount of DNA for amplification, there are 874 SSR markers assembled in ordered positions within the 20 soybean linkage groups. Thus, they have the potential to accelerate and simplify breeding efforts for soybean cultivars with improved flavor [13,14,15,20,21,22] .…”

Section: Introductionmentioning

confidence: 99%

Soybean Improvement for Lipoxygenase-free by Simple Sequence Repeat (SSR) Markers Selection

et al. 2021

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Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified. The SSR markers Sat090 and Sat417 was the most effective diagnostic marker among the all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in The F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.

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UEL 175: a novel lipoxygenase-free soybean cultivar with kunitz trypsin inhibitor absence

Abstract: -UEL 175 is lipoxygenase-free soybean cultivar and has absence of the antinutritional factors kunitz trypsin inhibitor (SKTI

Cited by 3 publications

References 5 publications

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

Enhancing the Nutritional Quality of Major Food Crops Through Conventional and Genomics-Assisted Breeding

Genetic elimination of lipoxygenase‐2 improves sprouting and tocopherols, and removal of Kunitz trypsin inhibitor enhances in vitro protein digestibility in soybean (Glycine max)

Soybean Improvement for Lipoxygenase-free by Simple Sequence Repeat (SSR) Markers Selection

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