Morphological effects of induced polyploidy in Dendrobium nobile Lindl. (Orchidaceae)

Vichiato, Marcelo; Pasqual, Moacir; Rodrigues, Filipe Almendagna; Castro, Daniel Melo de

doi:10.1590/1984-70332014v14n3a23

Cited by 32 publications

(36 citation statements)

References 11 publications

(11 reference statements)

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“…Although previous investigators did not find phenotypic differences in traits among cytotypes in P. spicata (Jones & Larson ), we found that P. spicata plants from tetraploid populations were larger than those from diploid populations. These observations are consistent with findings that polyploid cytotypes are often larger (Vichiato et al ) and more competitive than diploids (Thebault et al ). We also found that all measured traits varied significantly among populations from the same ploidy group, likely in response to local environment and climate (St Clair et al ; Gibson ).…”

Section: Discussionsupporting

confidence: 92%

Polyploidy: a missing link in the conversation about seed transfer of a commonly seeded native grass in western North America

Gibson

Fishman

Nelson

2016

Restoration Ecology

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The use of local, native plant materials is now common in restoration but testing for polyploidy in seed sources is not. Diversity in cytotypes across a landscape can pose special seed transfer challenges, because the methods used to determine genetically appropriate materials for seed transfer do not account for cytotypic variation. This lack of consideration may result in mixing cytotypes through revegetation, which could reduce long‐term population viability. We surveyed nine populations of a native bunchgrass, Pseudoroegneria spicata, in three EPA Level III Ecoregions in the western United States to determine the frequency of polyploidy, whether there are differences in traits (phenotype, fecundity, and mortality) among plants of different cytotypes, and whether cytotype frequency varies among ecoregions. We assessed trait variation over 2 years in a common garden and determined ploidy using flow cytometry. Polyploidy and mixed cytotype populations were common, and polyploids occurred in all ecoregions. Four of the nine populations were diploid. The other five had tetraploids present: three had only tetraploid individuals whereas two had mixed diploid/tetraploid cytotypes. There was significant variation in traits among cytotypes: plants from tetraploid populations were larger than diploid or mixed populations. The frequency and distribution of cytotypes make it likely that seed transfer in the study area will inadvertently mix diploid and polyploid cytotypes in this species. The increasing availability of flow cytometry may allow ploidy to be incorporated into native plant materials sourcing and seed transfer.

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Section: Discussionsupporting

confidence: 92%

Polyploidy: a missing link in the conversation about seed transfer of a commonly seeded native grass in western North America

Gibson

Fishman

Nelson

2016

Restoration Ecology

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“…Reduction in corm diameter and corm multiplication rate of treated plants is also one of the negative effects of colchicine treatment which could be the result of polyploidy induction. Decreased diameter of orchid (Dendrobium nobile) pseudobulbs as observed by Vichiato et al (2014) was due to induction of polyploidy and doubling of cell genomic material. It causes increase in utilization of stored food during different growth stages and flowering.…”

Section: Discussionmentioning

confidence: 99%

“…However late flowering in tetraploid orchid (Dendrobium nobile) plants could be associated with increased cell size and cell nuclear volume. As colchicine increases the cell size so these cells need more time and energy for a DNA duplication which reduced the rate of cell division (Vichiato et al, 2014). In brassica plants, (Brassica campestris) there was a delay of 11 days in 50% flowering in colchicine treated plants as compared to control plants (Kumar and Dwivedi, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, they are quite safe to handle in liquid medium (Lehrer et al, 2008). Colchicine is a chemical mutagen that is widely used for induction of polyploidy in various ornamental species like pelargonium (Pelargonium graveolens) (Jadrna et al, 2010), salvia (Salvia hians) (Grouh et al, 2011), Madgascar periwinkle (Catharanthus roseus) (Hosseini et al, 2013), orchid (Dendrobium nobile) (Vichiato et al, 2014), chrysanthemum (Dendranthema indicum) , Bougainvillea (Bougainvillea glabra) (Anitha et al, 2017), phlox (Phlox drummondii) (Dar et al, 2017) and swamp rosemallow (Hibiscus moscheutos) (Li and Ruter, 2017). Colchicine not only changes the chromosome number but also induces gene mutation in both seed and vegetatively propagated crops (Datta, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus ‘White Prosperity’

Manzoor

Ahmad

Bashir

et al. 2018

Folia Horticulturae

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Gladiolus is one of the most important lucrative cut flower crops that is commercially cultivated worldwide due to its various spike forms, size, and shape and color combinations. In order to further increase the commercial and horticultural value by improving the ornamental traits of gladiolus 'White Prosperity', polyploidy was induced by soaking gladiolus corms in different colchicine concentrations (0.1%, 0.2% and 0.3%) for 24 h. Different colchicine concentrations had a little effect on sprouting and survival percentage but it significantly delayed the emergence of sprouts. About one third decreases in plant height along with reduction in number of leaves per plant, leaf area, length and width, chlorophyll content, diameter and number of cormlets per corm was observed in treated plants. Colchicine at 0.1% concentration improved the ornamental value of gladiolus by increasing vase life whereas colchicine at 0.3% was effective in increasing floret diameter. However, the colchicine treated plants exhibited delayed and reduced percentage of flowering corms. Pollen and stomatal study was done for the identification of polyploidy and it showed that both pollen and stomata size were increased while stomatal density and pollen fertility was significantly reduced in polyploid plants. Induction of polyploidy (mixoploids + octoploids) was achieved in all concentrations, however 0.2% and 0.3% concentrations of colchicine were effective for producing large number of polyploid plants. But at 0.1% concentration of colchicine, majority of plants did not show any change in their original ploidy level (tetraploid). These putative polyploids may be helpful for further improvement in ornamental and horticultural value of gladiolus.Ke y wor d s: chromosome, diploid, pollen, stomata, tetraploid

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“…However, there have been few studies on breeding orchids due to the long cycle of plants in the Dendrobium genus i. e., which leads to an average flowering development of three to four years (Vichiato et al 2014). Faria et al (2009), Faria et al (2011), and Faria et al (2013, working with Dendrobium crosses, were successful in obtaining three new cultivars (UEL 6, UEL 7 and UEL 8), which expressed the desired characteristics of mother plants such as flower size, flower coloration, flourishing period, number of flowers, plant height, among others.…”

Section: Introductionmentioning

confidence: 99%

Cattleya forbesii x Cattleya bowringiana: a new hybrid of Cattleya orchid

Colombo

Hoshino

Ferrari

et al. 2017

Crop Breed. Appl. Biotechnol.

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Morphological effects of induced polyploidy in Dendrobium nobile Lindl. (Orchidaceae)

Abstract: -

Cited by 32 publications

References 11 publications

Polyploidy: a missing link in the conversation about seed transfer of a commonly seeded native grass in western North America

Polyploidy: a missing link in the conversation about seed transfer of a commonly seeded native grass in western North America

Induction and identification of colchicine induced polyploidy in Gladiolus grandiflorus ‘White Prosperity’

Cattleya forbesii x Cattleya bowringiana: a new hybrid of Cattleya orchid

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