A glutathione s-transferase confers herbicide tolerance in rice

Hu, Tingzhang

doi:10.1590/1984-70332014v14n2a14

Cited by 27 publications

(20 citation statements)

References 23 publications

(18 reference statements)

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“…These results are in agreement with previous studies, according to which safeners significantly change the affinity of GST to substrate of conjugation reaction (Scarponi et al, 2006). It has also been observed that high GST activity may contribute to the tolerance of rice to glyphosate and chlorsulfuron (Hu, 2014). A positive correlation between growth level and GSH content, GST activity has been observed in this research.…”

Section: Discussionsupporting

confidence: 93%

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Fei

Cao

et al. 2016

Zemdirbyste-Agriculture

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Herbicide safener, a diverse group of chemicals, is an important tool used to protect plants from herbicidal injury. With the aim of decreasing drift injury of haloxyfop-P-methyl to sensitive plants, the protective effect of four safeners (R-28725, 3-dichloroacetyl oxazolidine and its two optical isomers) was evaluated. Physiological and biochemical tests were conducted under laboratory conditions in Northeast Agricultural University, China, by using seed treatment with safener and soil treatment with haloxyfop-P-methyl, respectively. The maize seeds treated with these safeners were safe from haloxyfop-P-methyl treatment. A positive correlation between growth level and endogenous glutathione (GSH) content, glutathione S-transferases (GST) activity was observed in this research. Enhancement of GSH content, GST activity and affinity of GST to 1-chloro-2,4-dinitrobenzene (CDNB) in maize treated by R-28725 was maximum. However, the detoxification of herbicide was not accompanied by the increase of acetyl-CoA-carboxylase (ACC) activity in maize.

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Section: Discussionsupporting

confidence: 93%

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Fei

Cao

et al. 2016

Zemdirbyste-Agriculture

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“…The GSTF1 gene conferred greater tolerance to the herbicides chlorotoluron and fenoxaprop-P-ethyl in A. myosuroides and L. rigidum, causing multiple resistance (Cummins et al, 2013). In rice, overexpression of the GSTL1 or GSTL2 genes led to greater tolerance to chlorsulfuron and glyphosate (Hu, Qv, Xiao, & Huang, 2009;Hu, 2014). Likewise, the greater expression of the GST1 gene has been related with resistance of E. crus-galli plants to quinclorac (Li et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Genes Associated With Degradation Enhancement of Imazethapyr in Barnyardgrass (Echinochloa crus-galli)

Dalazen¹,

Markus²,

Merotto³

2018

JAS

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The understanding of mechanism of herbicide resistance in weeds is essential for adequate or innovative weed management practices. The aim of this study was to identify and analyze the expression of genes related to degradation enhancement of imazethapyr in barnyardgrass (Echinochloa crus-galli L. Beauv.). One susceptible (SUSSP01) and two populations previouslly identified as resistant to imazethapyr (ARRGR01 and PALMS01) were used. Gene expression of CYP and GST, the translation initiating factor eIF4B, and ALS genes were evaluated after imazethapyr spraying. A reference gene stability analysis was carried out, wherein the genes 18S and actin showed to be more stable in response to the population and herbicide treatment. The gene expression analysis was performed by qRT-PCR. There was no difference in the relative expression of the ALS gene. The CYP81A6 and GSTF1 genes showed higher relative expression in the resistant populations. The CYP81A6 gene had expression 9.61 and 8.44 higher in the resistant populations ARRGR01 and PALMS01, respectively, in comparison with the untreated susceptible population. The expression of this gene was induced by spraying the herbicide imazethapyr. The GSTF1 gene showed higher relative expression in PALMS01 population, reaching 12.30 times higher in plants treated with imazethapyr in relation to untreated susceptible population. The expression of eIF4B gene in the resistant populations treated with imazethapyr was about six times higher than observed in susceptible population. The high relative expression of CYP81A6 and GSTF1 genes indicate the importance of degradation enhancement for the resistance of barnyargrass to imazethapyr.

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“…1). The detoxification and antioxidant activity of GSTs in barley has been reported by Rezaei et al [39]. Rice GSTs have been reported with biological role in detoxification of xenobiotic and abiotic stress tolerance [40,41] in transgenic plants.…”

Section: Comparative Investigation Of Gst Activities In Different Cropsmentioning

confidence: 82%

Comparative Investigation of Glutathione S-transferase (GST) in Different Crops and Purification of High Active GSTs from Onion (Allium cepa L.)

Islam¹

2015

JPS

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Glutathione S-transferases (GSTs; EC 2.5.1.18) are abundant proteins encoded by a highly divergent ancient gene family with protective functions through detoxification and non-catalytic roles as carriers of cytotoxins. In this work, we investigated the GST activities in seedlings of 38 crops to obtain a plant with high active GST for further study. In screening of 38 crops, onion seedling showed the highest GST activity (483.54 nmol min -1 mg -1 protein) followed by wheat(372.89 nmol min -1 mg -1 protein), barley (253.44 nmol min -1 mg -1 protein), rice (244.12 nmol min -1 mg -1 protein) and proso millet (173.34 nmol min -1 mg -1 protein). Carrot seedling showed the lowest activity (3.63 nmol min -1 mg -1 protein).In onion plants, both root and leaf showed high GST activity. Onion bulb GSTs were separated DEAE cellulose column chromatography, and purified by affinity chromatography (S-hexyl glutathione-agarose). Three GST peaks were found to elute at 67, 107 and 140mM of KCl gradient solution, and were named as GSTa, GSTb and GSTc. Among the three GSTs, GSTa, GSTb and GSTc contained 9.58, 61.45 and 28.97% of total activity, respectively. In purification, GSTa, GSTb and GSTc had specific activities of 9075, 17259 and 19868 nmol min -1 mg -1 protein, respectively, along with yield of 2.48, 3.17 and 1.28,and purification fold of 15.8, 29.9 and 34.5, respectively. The purity and molecular mass of the fraction was examined by SDS-PAGE. The silver staining of the purified GSTa, GSTb and GSTc indicated that final product of GSTb and GSTc were highly purified and migrated as a single band on SDS-PAGE with an apparent molecular mass of 27 kDa. However, GSTa eluted with Glyoxalase-I (Gly-I) and to purify GSTa, more methodological application are suggested.

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A glutathione s-transferase confers herbicide tolerance in rice

Abstract: -Plant glutathione S-transferases (GSTs

Cited by 27 publications

References 23 publications

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

The safener effect of chiral derivatives of 3-dichloroacetyl oxazolidine against haloxyfop-P-methyl-induced toxicity in maize

Differential Expression of Genes Associated With Degradation Enhancement of Imazethapyr in Barnyardgrass (Echinochloa crus-galli)

Comparative Investigation of Glutathione S-transferase (GST) in Different Crops and Purification of High Active GSTs from Onion (Allium cepa L.)

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