The effects of cryopreservation on the acrosome structure, enzyme activity, motility, and fertility of bovine, ovine, and goat sperm

Sun, Wei; Jiang, Shan; Su, Jie; Zhang, Jia; Bao, Xiangnan; Ding, Rui; Shi, Peixin; Li, Shufang; Wu, Chong; Zhao, Gaoping; Cao, Guifang; Sun, Qing‐Yuan; Yu, Hao; Li, Xihe

doi:10.1590/1984-3143-ar2020-0219

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…Finally, proteomic profiling of frozen–thawed human spermatozoa [ 101 ] unraveled a significant upregulation of several proteins including clusterin (CLU) as a major sperm glycoprotein [ 133 ], tektin (TEKT) 2 and 3 present in the axoneme and periaxonemal structures necessary for progressive motility of spermatozoa [ 134 ], and L-xylulose reductase (DCXR), a multifunctional protein involved in various enzymatic and protein interaction processes [ 135 ]. On the other hand, proteins such as acrosin (ACR), the major proteinase present in the acrosome of mature spermatozoa [ 136 ], calmodulin (CALM1) involved in calcium signal transduction pathways [ 137 ], cytochrome (CYC2), and NADH-cytochrome b5 reductase2 (CYB5R2) as critical elements involved in sperm mitochondrial biogenesis and metabolism [ 138 , 139 ] were significantly decreased following cryopreservation, resulting in reduced post-thaw semen quality. A summary of the most studied sperm protein markers associated with sperm freezability is provided in Table 2 .…”

Section: Protein Markersmentioning

confidence: 99%

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Ďuračka

Benko

Tvrdá

2023

IJMS

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For decades now, sperm cryopreservation has been a pillar of assisted reproduction in animals as well as humans. Nevertheless, the success of cryopreservation varies across species, seasons, and latitudes and even within the same individual. With the dawn of progressive analytical techniques in the field of genomics, proteomics, and metabolomics, new options for a more accurate semen quality assessment have become available. This review summarizes currently available information on specific molecular characteristics of spermatozoa that could predict their cryotolerance before the freezing process. Understanding the changes in sperm biology as a result of their exposure to low temperatures may contribute to the development and implementation of appropriate measures to assure high post-thaw sperm quality. Furthermore, an early prediction of cryotolerance or cryosensitivity may lead to the establishment of customized protocols interconnecting adequate sperm processing procedures, freezing techniques, and cryosupplements that are most feasible for the individual needs of the ejaculate.

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Section: Protein Markersmentioning

confidence: 99%

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Ďuračka

Benko

Tvrdá

2023

IJMS

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“…Cryopreservation can cause lipid peroxidation in the sperm membrane and induce an enhancement in reactive oxygen species concentration [ 228 ]. Elevated levels of reactive oxygen species (ROS) compromise sperm motility [ 229 , 230 , 231 ] and viability [ 232 ], DNA integrity [ 233 ], and acrosomal structure [ 234 , 235 ]. Cryopreservation leads to sperm apoptosis, affecting mitochondrial membrane properties and enhancing ROS generation [ 236 ] (see Table 2 ).…”

Section: New Therapeutical Opportunitiesmentioning

confidence: 99%

NGF and the Male Reproductive System: Potential Clinical Applications in Infertility

Ferraguti

Fanfarillo

Tarani

et al. 2022

IJMS

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Infertility is a worldwide health issue defined by the World Health Organization (WHO) as the inability to establish a pregnancy after 12 months or more of regular and unprotected sexual intercourse. Male infertility etiology can be related to either congenital or acquired factors. The therapeutical approach to male infertility depends on the underlying causes and includes medical and surgical treatments. In recent studies, the potential role of nerve growth factor (NGF) in male reproductive physiology has been proposed. It has been hypothesized that neurotrophins might be involved in testis morphogenesis and regulation of several aspects of spermatogenesis. Moreover, it has been shown that NGF exerts its role on gonadotropin-releasing hormone (GnRH) neurons through the activation of the PKC/p–ERK1/2/p–CREB cascade, which leads to the activation of hypothalamic cells and the consequent activation of hypothalamus–pituitary–gonadal axis (HPG) with the secretion of GnRH. Lastly, it has been shown that the physiology of mature sperm is affected by both exogenous and endogenous NGF. The NGF impact on the HPG axis and its effect on GnRH neurons might be exploited in the therapy of male hypogonadism or used as a protective strategy against gonadal dysfunction related to chemotherapeutic agents. Moreover, the improving effect of NGF on sperm motility and vitality could be useful to enhance assisted reproduction outcomes. NGF could be supplemented to cryopreserved sperm samples to counteract the oxidative stress induced by the frozen and thawing processes. Indeed, the potential clinical applications of NGF in male infertility treatment have been discussed.

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“…Due to its small molecular weight, Giemsa staining can bind to proteins on the membrane and can pass through cell membranes that protect the acrosomes (Nofa et al, 2018;Prihantoko et al, 2020). Furthermore, the integrity acrosome is needed to ensure the success of spermatozoa in fertilizing the egg because the cap protects the enzymes contained (Sun et al, 2020).…”

Section: Plasma Membrane and Intact Acrosomementioning

confidence: 99%

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

Tethool¹,

Ciptadi²,

Wahjuningsih³

et al. 2022

WVJ

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Frozen semen is produced through several stages, which deteriorate spermatozoa. This research aimed to evaluate the deterioration degree of frozen semen after 5 °C cooling and freezing of Bali cattle. The samples included 10 male Bali cattle with a body weight of 542-668 kg, from which semen was collected once a week for five weeks. The deterioration of each individual’s sperm was determined by observing two distinct straws. The parameters observed included viability, abnormalities, intact plasma membrane, and intact acrosome cap. Initial observations of the parameters were conducted following the addition of semen to diluent A1 (AD) as much as the volume of fresh semen. The semen in the AD group was not cooled and frozen. The A1 semen was then divided into two, namely, those with cooling at 5 °C for 4 hours (PT1) and at 5°C for 22 hours (PT2). The results showed that individual variations in Bali cattle caused significant differences in viability and intact plasma membrane of AD and PT1 groups, while PT2 did not differ in viability and intact plasma membrane spermatozoa. Abnormalities were significantly different between AD and PT2 groups, however PT1 did not differ in abnormalities spermatozoa. Intact acrosomal cap was significantly different in the AD, PT1, and PT2 groups. In conclusion, individual variations, including viability, abnormalities, intact plasma membrane, and acrosome cap of spermatozoa, were better at 4 hours compared to cooling at 5°C for 22 hours. A Cooling time of 4 hours at 5°C can be recommended for frozen semen processing.

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The effects of cryopreservation on the acrosome structure, enzyme activity, motility, and fertility of bovine, ovine, and goat sperm

Cited by 10 publications

References 18 publications

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability

NGF and the Male Reproductive System: Potential Clinical Applications in Infertility

Deterioration of Frozen Semen of Bali Cattle after Cooling at 5°C

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