In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan, Joanna Maria Gonçalves; Leal, Gabriela Ramos; Monteiro, Clara Ana Santos; Batista, Ribrio Ivan Tavares Pereira; Barbosa, Nathalia Oliveira; Freitas, Vicente José Figueirêdo

doi:10.1590/1984-3143-ar2023-0055

Cited by 1 publication

(1 citation statement)

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“…Recent estimates in 2021 indicated that the maturation rate of sheep is 59%-92%, and the blastocyst rate is only 14%-33%. IVP is still the leading technology for small ruminants; however, outcomes are influenced by many factors (Souza-Fabjan et al, 2023). Optimizing the embryo culture system is important.…”

Section: Introductionmentioning

confidence: 99%

Effects of Leonurine on oocyte maturation and parthenogenetic embryo development in sheep

Wei,

Wang,

et al. 2024

Reprod Domestic Animals

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Leonurine (LEO), an alkaloid isolated from Leonurus spp., has anti‐oxidant, anti‐inflammatory and anti‐apoptotic effects and can prevent damage caused by reactive oxygen species (ROS). These properties suggest that it can improve the maturation rate of oocytes and developmental ability of embryos, which are key parameters in animal breeding. In this study, the effects of LEO on in vitro maturation and early embryonic development in sheep oocytes were evaluated. Among various doses examined (0, 10, 20 and 40 μM), a dose of 20 μM was optimal with respect to the oocyte maturation rate. Compared with estimates in the control group, GSH levels and mitochondrial membrane potential of sheep oocytes treated with 20 μM LEO were significantly higher, and 40 μM LEO would affect oocyte maturation. Additionally, ROS levels were significantly lower, expression levels of the antioxidant genes CAT and SOD1 were significantly higher, and there was no significant difference in GPX3 expression. The Bax/Bcl‐2 ratio and Caspase‐3 expression were significantly reduced in the 20 μM LEO group. During early embryonic development in vitro, the cleavage rate and blastocyst rate were significantly higher in the 20 μM LEO treatment group compared to other groups. GSH levels and mitochondrial membrane potential were significantly higher, while ROS levels were significantly lower, and expression levels of the antioxidant genes CAT, GPX3 and SOD1 were significantly higher in eight‐cell embryos treated with 20 μM LEO than in the control group. The Bax/Bcl‐2 ratio and Caspase‐3 levels were significantly decreased. In summary, LEO can reduce the effect of oxidative stress, improve the oocyte maturation rate and enhance embryonic development.

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Section: Introductionmentioning

confidence: 99%