Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

Mustofa, Imam; Susilowati, Suherni; Suprayogi, Tri Wahyu; Oktanella, Yudit; Purwanto, Djoko; Akintunde, Adeyinka Oye

doi:10.1590/1984-3143-ar2022-0025

Cited by 2 publications

References 51 publications

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Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Yang,

Geng,

Duan

et al. 2023

Preprint

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Background: Although sperm DNA fragmentation index (DFI) is an vital biomarker for sperm DNA integrity and male fertility, factors that influence DFI have not been fully elucidated, particularly for occupational exposures and lifestyle factors. Here, we sought to explore the effects of self-reported occupational exposures and lifestyle factors on sperm DNA integrity. Methods: The baseline characteristics, occupational exposures, and lifestyle factors of participants were collected immediately prior to or following semen evaluation through a self-report questionnaire. Participants were separated into three DFI-based groups: DFI<15%, 15%≤ DFI<30%, and 30%≤DFI. Initially, one-way ANOVAs and chi-square tests were employed to investigate the possible factors related to sperm DNA integrity. Then, we performed an ordered logistic regression analysis to detect factors independently associated with sperm DFI. To adjust for relevant confounders, we included factors with a P< 0.25 in univariate analyses as covariates. Results: Among 1,060 subjects who participated in this study, 826 met the icludion cretieria. Univariate analysis showed that age, infertility type, infertility duration, semen parameters (concentration, motility and morphology) and smoking were related to sperm DNA integrity (P<0.05). At ordered logistic regression analysis, compared with the group that drank green tea 5-7 times/week, the groups that drank green tea <1 time/week (OR=1.52, 95% CI=1.05-2.2, and p=0.027) and 1-4 times/week (OR=1.61, 95% CI=1.02-2.54, and p=0.042) exhibited significantly increased sperm DFI values. Of the traditional semen parameters measured, a positive correlation was noted between sperm DFI and semen volume (OR=1.18, 95% CI=1.07-1.31) and abnormal sperm morphology (OR=1.2, 95% CI=1.11-1.3), whereas it was negatively correlated with sperm motility (OR=0.94, 95% CI=0.93-0.95). Relative to men over 40 years of age, we detected a 65% (95% CI=0.2-0.62) and 58% (95% CI=0.26-0.7) decrease in sperm DFI values in the 20-30 and 30-40 age groups, respectively. Conclusions: These data suggest that green tea consumption is a protective factor associated with the preservation of sperm DNA integrity, which may offer new insights to guide the prevention and treatment of clinical male infertility.

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Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Yang,

Geng,

Duan

et al. 2023

Preprint

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Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

Susilowati,

Mustofa,

Suparyogi

et al. 2024

Asian Pacific Journal of Reproduction

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Objective: To improve the quality of post-thawing Boer buck semen for artificial insemination by adding green tea extract chitosan nanoparticles to skimmed egg yolk diluent, and the proper thawing temperature. Methods: The ejaculate of Boer buck was added to skimmed egg yolk diluent without (the control group) and with adding 1 μg of chitosan nanoparticles of green tea extract per mL of diluent (the treatment group). Then, the diluted semen was filled in French mini straws containing 60× 106 live sperm per straw, frozen in a standard protocol, and stored as frozen semen at -196 °C for a week. Six replicates from each group were diluted for 30 s at 37 °C or 39 °C sterile water to evaluate the semen quality. Results: Post-thawing (at 37 °C or 39 °C) of live sperm, progressive motility, and plasma membrane integrity were lower compared to those of the pre-freezing stage (P<0.05). Thawing at 37 °C resulted in no significant difference in live sperm, progressive motility, and plasma membrane between the control group and the treatment group (P>0.05). The live sperm, progressive motility, and plasma membrane of the treatment group in the pre-freezing stage, and post-thawed at 39 C were higher compared to those of the control group (P<0.05). There was no significant difference in malondialdehyde (MDA) concentration, DNA fragmentation, and catalase concentration of thawing at 37 °C compared to those of 39 °C in the same group. The MDA concentration and DNA fragmentation in thawing at 37 °C and 39 °C of the treatment group were significantly lower than those of the control group (P<0.05). However, the catalase concentration in thawing at 37 °C and 39 °C of the treatment group was not significantly different than the control group (P>0.05). Conclusions: Higher quality post-thawing Boer buck semen is achieved by adding 1 μg/mL of chitosan nanoparticles of green tea extract to the skimmed egg yolk diluent and thawing at 39 °C.

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Combination of nanoparticle green tea extract in tris-egg yolk extender and 39 °c thawing temperatures improve the sperm quality of post-thawed Kacang goat semen

Cited by 2 publications

References 51 publications

Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Adding chitosan nanoparticles of green tea extract in diluent and thawing temperatures ameliorate the post-thawed quality of Boer buck semen

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