The embryo culture media in the era of epigenetics: is it time to go back to nature?

Coy, Pilar; Romar, Raquel; Romero-Aguirregomezcorta, Jon

doi:10.1590/1984-3143-ar2021-0132

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…According to the Developmental Origin of Health and Disease (DOHaD) hypothesis [ 28 ], the differences in the environment during the preimplantation period in the two groups of animals can draw marks in the epigenome of the embryos, such as alterations in the DNA methylation patterns [ 29 ],whose consequences are expressed later in development. It is well known that the plasticity embryos lets them adapt to stressful conditions such as those derived from the in vitro culture, and it has been shown in mice that the long-term programming of postnatal development, growth, physiology, and behavior is affected irreversibly by preimplantation conditions [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Differences in Weight, Hierarchy, and Incidence of Lameness between Two Groups of Adult Pigs Derived from Assisted Reproductive Technologies

Romero-Aguirregomezcorta

Ramírez

Ortín

et al. 2022

Animals

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The in vitro production (IVP) and subsequent transfer of embryos (ET) to recipient mothers is not yet an established reproductive technology in the pig industry, as it is in cattle. However, that the trade of IVP-cryopreserved pig embryos is expected to start in the next decades. Society and governments are increasingly aware of the repercussions that IVP could have for animal health, welfare, behavior, or food safety, but proven scientific information for this type of animal does not exist, since no colonies of pigs have been created to this end. We created a small one and studied the differences between 16 IVP-derived pigs and 14 pigs derived from artificial insemination (AI), at 3.5 years of age, conceived from the same boar, and housed and fed under the same conditions since they were born. Incidence of lameness, position in the herd hierarchy, weight, adenosine deaminase activity, and hematological and biochemical analytes were compared between the two groups of animals. The results showed that the IVP animals weighed more, occupied higher positions in the herd hierarchy, and had a lower incidence of lameness. Although genetic differences from the maternal line could explain some of these results, it is also possible that the IVP animals developed better adaptative abilities, but more studies with a higher number of animals are necessary to reach consistent conclusions.

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Section: Discussionmentioning

confidence: 99%

Differences in Weight, Hierarchy, and Incidence of Lameness between Two Groups of Adult Pigs Derived from Assisted Reproductive Technologies

Romero-Aguirregomezcorta

Ramírez

Ortín

et al. 2022

Animals

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“…After ovulation the oocyte moves to the oviduct, where fertilization and early embryo development take place. Oviductal fluid is secreted by the oviduct and acts as an embryotropic culture media for the oocyte and early embryo for their time in residence ( 13 , 14 ). Addition of Se to in vitro fertilization cultures in animal models (cattle, dogs, pigs, yak, etc.)…”

Section: Selenium In the Female Reproductive Tractmentioning

confidence: 99%

Selenium supplementation and pregnancy outcomes

Dahlen

Reynolds²,

Caton³

2022

Front. Nutr.

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In vertebrates and invertebrates, selenium (Se) is an essential micronutrient, and Se deficiency or excess is associated with gonadal insufficiency and gamete dysfunction in both males and females, leading to implantation failure, altered embryonic development and, ultimately, infertility. During pregnancy, Se excess or deficiency is associated with miscarriage, pre-eclampsia (hypertension of pregnancy), gestational diabetes, fetal growth restriction and preterm birth. None of this is surprising, as Se is present in high concentrations in the ovary and testes, and work in animal models has shown that addition of Se to culture media improves embryo development and survival in vitro in association with reduced reactive oxygen species and less DNA damage. Selenium also affects uterine function and conceptus growth and gene expression, again in association with its antioxidant properties. Similarly, Se improves testicular function including sperm count, morphology and motility, and fertility. In animal models, supplementation of Se in the maternal diet during early pregnancy improves fetal substrate supply and alters fetal somatic and organ growth. Supplementation of Se throughout pregnancy in cows and sheep that are receiving an inadequate or excess dietary intake affected maternal whole-body and organ growth and vascular development, and also affected expression of angiogenic factors in maternal and fetal organs. Supplemental Se throughout pregnancy also affected placental growth, which may partly explain its effects on fetal growth and development, and also affected mammary gland development, colostrum yield and composition as well as postnatal development of the offspring. In conclusion, Se supplementation in nutritionally compromised pregnancies can potentially improve fertility and pregnancy outcomes, and thereby improve postnatal growth and development. Future research efforts should examine in more detail and more species the potential benefits of Se supplementation to reproductive processes in mammals.

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“…Debido a los problemas que presenta el SFB en los medios de cultivo embrionario, se debe buscar otra alternativa para que los embriones producidos in vitro tengan un ambiente lo más fisiológico posible y para superar la baja eficiencia que tienen las técnicas de reproducción asistida (Coy et al, 2022). De esta manera, el ambiente al que se exponen en el laboratorio sería menos estresante.…”

Section: Introductionunclassified

Efecto De Las Secreciones De Organoides De Endometrio Humano Sobre El Cultivo in Vitro De Embriones Bovinos

Masegosa Domínguez,

Romero-Aguirregomezcorta,

Juan

et al. 2024

An. Vet. Murcia

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Over the last 50 years, the fertility of high-producing dairy cows has declined due to the intensity of genetic selection. To overcome this obstacle, assisted reproductive techniques have been used. However, in vitro embryo production is a relatively inefficient process, with maximum blastocyst yield rates of around 35%. To overcome this problem, it was hypothesised that by adding endometrial organoid secretions to the culture medium, embryos produced in vitro would be exposed to a more physiological environment and the yield of the technique would increase. For this purpose, bovine oocytes were collected from a local abattoir and matured in vitro, fertilised in vitro and cultured in vitro. The embryo culture medium was supplemented at 1% (v/v) with i) human endometrial organoid secretions (as bovine secretions are not available), ii) Boretto culture medium, and ii) matrigel drops with Boretto culture medium, as procedural controls. In addition, a control group without any supplementation was included. Embryo development was assessed at days 2, 7 and 8 of culture. The percentage of blastocysts at day 7 and day 8 post insemination was significantly higher (P<0.05) when Boretto culture medium was added. In addition, this treatment increased embryo development kinetics at day 7 post insemination, and blastocyst quality as assessed by total cell number, with 85.8% more than the control. Although human endometrial secretions have not given the expected result, it would be necessary to test whether bovine endometrial-specific organoid secretions, at different concentrations, could increase the yield of bovine embryo production in vitro. Durante los últimos 50 años, la fertilidad de las vacas lecheras de alta producción ha disminuido debido a la intensidad de la selección genética. Para superar este obstáculo, se ha recurrido a las técnicas de reproducción asistida. Sin embargo, la producción de embriones in vitro es un proceso relativamente ineficiente, con tasas máximas de blastocistos obtenidos de alrededor del 35%. Para solventar este problema, se planteó la hipótesis de añadir al medio de cultivo secreciones de organoides de endometrio, así, los embriones producidos in vitro estarían expuestos a un ambiente más fisiológico y el rendimiento de la técnica aumentaría. Para ello, se llevó a cabo la recolección de ovocitos bovinos procedentes de un matadero local, y se procedió a su maduración in vitro, fecundación in vitro y cultivo embrionario in vitro. El medio de cultivo embrionario se suplementó al 1% (v/v) con i) secreciones de organoides de endometrio humano (ya que no existe disponibilidad de secreciones bovinas), ii) medio de cultivo Boretto, y ii) gotas de matrigel con medio de cultivo Boretto, como controles de procedimiento. Además, se incluyó un grupo control sin ningún suplemento. Se evaluó el desarrollo embrionario a los días 2, 7 y 8 de cultivo. El porcentaje de blastocistos a día 7 y a día 8 post inseminación fue significativamente mayor (P<0.05) cuando se añadió el medio de cultivo Boretto. Además, este tratamiento incrementó la cinética de desarrollo de los embriones a día 7 post inseminación, y la calidad de los blastocistos evaluada por el número total de células, con un 85.8% más que el control. Aunque las secreciones de endometrio humano no han dado el resultado esperado, sería necesario comprobar si las secreciones de organoides específicas de endometrio bovino, a distintas concentraciones, podrían incrementar el rendimiento de la producción in vitro de embriones bovinos.

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The embryo culture media in the era of epigenetics: is it time to go back to nature?

Cited by 6 publications

References 48 publications

Differences in Weight, Hierarchy, and Incidence of Lameness between Two Groups of Adult Pigs Derived from Assisted Reproductive Technologies

Differences in Weight, Hierarchy, and Incidence of Lameness between Two Groups of Adult Pigs Derived from Assisted Reproductive Technologies

Selenium supplementation and pregnancy outcomes

Efecto De Las Secreciones De Organoides De Endometrio Humano Sobre El Cultivo in Vitro De Embriones Bovinos

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