Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts

Schimming, Bruno César; Martins, Leandro Luís; Oliveira, Fabrício Singaretti de; Pinheiro, Patrícia Fernanda Felipe; Domeniconi, Raquel Fantin

doi:10.1590/1984-3143-ar2021-0070

Cited by 2 publications

(3 citation statements)

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“…Moreover, while principal cells form the blood-epididymal barrier (Cornwall 2009) Ibex, mouflon, and chamois epididymides showed positive AQP9 immunostaining in principal, apical, and basal cells in caput and corpus regions and principal and apical cells in the cauda region. Sheep (Schimming et al 2015) and Azara's agouti (Schimming et al 2021) showed a similar AQP9 expression pattern but in a nuclear location instead of the membrane location of the three wild ungulates. However, in ram cauda epididymis, the apical epithelial lining, including microvilli, was also positive for AQP9.…”

Section: Discussionmentioning

confidence: 82%

“…Immunolocalisation of aquaglyceroporins in the epithelial epididymis has already been identified in human (Pastor-Soler et al 2001;Mobasheri et al 2005), rat (Pastor-Soler et al 2001;Badran and Hermo 2002;Hermo et al 2004Hermo et al , 2008Da Silva et al 2006), buffalo (Arrighi et al 2016), sheep (Schimming et al 2015), horse (Klein et al 2013), pig (Schimming et al 2017), dog (Domeniconi et al 2008;Squillacioti et al 2021), cat (Arrighi and Aralla 2014), two bat species (Oliveira et al 2013;Castro, Kim et al 2017) and two wild rodent species (Menezes et al 2018;Schimming et al 2021). There are still no reports on AQPs immunolocalisation in the epididymis of Iberian ibex, mouflon, and chamois.…”

Section: Introductionmentioning

confidence: 99%

“…AQP9 is the most abundant aquaglyceroporin isoform in the epididymis; it has been reported in human(Pastor-Soler et al 2001), rat (Pastor-Soler et al. 2001Badran and Hermo 2002;Da Silva et al 2006;Hermo et al 2008), horse(Klein et al 2013), buffalo(Arrighi et al 2016), sheep(Schimming et al 2015), pig(Schimming et al 2017), dog(Squillacioti et al 2021), cat(Arrighi and Aralla 2014), common vampire (Desmodus rotundus)(Castro, Kim et al 2017), great fruit-eating bat (Artibeus lituratus)(Oliveira et al 2013), Azaras'agouti (Dasyprocta azarae)(Schimming et al 2021), and black-footed colilargo (Oligoryzomys nigripes)(Menezes et al 2018).…”

mentioning

confidence: 99%

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Immunolocalisation of aquaporins 3, 7, 9 and 10 in the epididymis of three wild ruminant species (Iberian ibex, mouflon and chamois) and sperm cryoresistance

Martinez-Madrid,

Martínez-Cáceres,

Pequeño

et al. 2023

Reprod. Fertil. Dev.

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Context In the epididymis, epithelial cells manage changes in the luminal environment for proper sperm maturation. Moreover, aquaglyceroporins, a subgroup of aquaporins (AQP), modulate the transport of water, glycerol and other small molecules in epithelial cells. Aims We aim to characterise the lining epithelium, quantify its cell composition and immunolocalise the aquaglyceroporins AQP3, AQP7, AQP9 and AQP10 alongside the epididymal ductus of three wild ruminant species, and to determine if species-specific differences could be associated with cauda sperm cryoresistance variations. Methods Epididymides from Iberian ibex (n = 5), mouflon (n = 5) and chamois (n = 6) were obtained. Cauda spermatozoa were collected and sperm parameters were analysed before and after freezing. Histology and immunohistochemistry of AQP3, 7, 9, 10 and T-CD3 were performed in the caput, corpus and cauda epididymal regions. Key results This work first describes the lining epithelium in Iberian ibex, mouflon and chamois epididymis along the three anatomical regions, consisting of principal, basal, apical, clear and halo cells. However, the percentage of each cell type differed in ibex compared to mouflon and chamois. The positive T-CD3 immunolabeling of all the halo cells confirmed their T-lymphocyte nature. Aquaglyceroporin expression patterns were similar among species, except for differences in AQP7 and AQP10 immunolocalisation in ibex. Species-specific differences in epididymal sperm cryoresistance were confirmed. Conclusions The epididymal epithelium of the three wild ruminants differ in their relative number of cell types and AQP immunolocalisation, which ultimately appears to affect cauda epidydimal spermatozoa cryoresistance. Implications Our study provides information on the relevance of the quantitative composition and AQP pattern expression in epididymal lining epithelium on sperm cryoresistance.

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Section: Discussionmentioning

confidence: 82%